Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food
Аутори
Dmitric, MarkoVidanovic, Dejan
Matovic, Kazimir
Tesovic, Bojana
Sekler, Milanko
Vićić, Ivan
Karabasil, Nedjeljko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.
Кључне речи:
molecular detection / food safety / validation / pathogen / artificial contaminationИзвор:
Czech Journal of Food Sciences, 2023, 41, 4, 287-294Издавач:
- Czech Academy of Agricultural Sciences
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Dmitric, Marko AU - Vidanovic, Dejan AU - Matovic, Kazimir AU - Tesovic, Bojana AU - Sekler, Milanko AU - Vićić, Ivan AU - Karabasil, Nedjeljko PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3234 AB - In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food. PB - Czech Academy of Agricultural Sciences T2 - Czech Journal of Food Sciences T1 - Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food VL - 41 IS - 4 SP - 287 EP - 294 DO - 10.17221/114/2022-CJFS ER -
@article{ author = "Dmitric, Marko and Vidanovic, Dejan and Matovic, Kazimir and Tesovic, Bojana and Sekler, Milanko and Vićić, Ivan and Karabasil, Nedjeljko", year = "2023", abstract = "In this study, the primers and the probe were designed, and a completely new real-time polymerase chain reaction (PCR) protocol for detecting the invA gene of Salmonella in food was optimised and validated in-house. The inclusivity test used 76 different Salmonella isolates with no false-negative results. The exclusivity was tested using 45 non-Salmonella microorganisms with no false-positive results. The method was also successfully applied while examining five different artificially contaminated food categories. The results were compared to the standard method (ISO 6579-1) and two previously validated real-time PCR methods. The developed assay is sensitive and specific for rapidly detecting Salmonella in food.", publisher = "Czech Academy of Agricultural Sciences", journal = "Czech Journal of Food Sciences", title = "Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food", volume = "41", number = "4", pages = "287-294", doi = "10.17221/114/2022-CJFS" }
Dmitric, M., Vidanovic, D., Matovic, K., Tesovic, B., Sekler, M., Vićić, I.,& Karabasil, N.. (2023). Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food. in Czech Journal of Food Sciences Czech Academy of Agricultural Sciences., 41(4), 287-294. https://doi.org/10.17221/114/2022-CJFS
Dmitric M, Vidanovic D, Matovic K, Tesovic B, Sekler M, Vićić I, Karabasil N. Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food. in Czech Journal of Food Sciences. 2023;41(4):287-294. doi:10.17221/114/2022-CJFS .
Dmitric, Marko, Vidanovic, Dejan, Matovic, Kazimir, Tesovic, Bojana, Sekler, Milanko, Vićić, Ivan, Karabasil, Nedjeljko, "Development of a novel invA gene-based real-time PCR assay for the detection of Salmonella in food" in Czech Journal of Food Sciences, 41, no. 4 (2023):287-294, https://doi.org/10.17221/114/2022-CJFS . .