Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
Само за регистроване кориснике
2009
Аутори
Bajić, VladanStanimirović, Zoran
Stevanović, Jevrosima
Spremo-Potparević, Biljana
Zivković, L.
Milicević, Z.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of respon...se when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
Кључне речи:
8-Cl-cAMP / antitumor agents / micronuclei / premature centromere separationИзвор:
Journal of BUON, 2009, 14, 1, 71-77Издавач:
- Balkan Union of Oncology (B.U.ON.)
Финансирање / пројекти:
- Евалуација дејства хормона и цитостатика прменом цитогенетичких анализа и Комет теста (RS-143018)
- Екофизиолошка и генетичка истраживања домаћих животиња и пчела у функцији повећања репродуктивних својстава и отпорности на болести (RS-143022)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Bajić, Vladan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Spremo-Potparević, Biljana AU - Zivković, L. AU - Milicević, Z. PY - 2009 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/614 AB - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of BUON T1 - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes VL - 14 IS - 1 SP - 71 EP - 77 UR - https://hdl.handle.net/21.15107/rcub_veterinar_614 ER -
@article{ author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Spremo-Potparević, Biljana and Zivković, L. and Milicević, Z.", year = "2009", abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3′,5′ cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assay (CA) on human peripheral blood lymphocytes of healthy individuals and by bone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 μm) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p.) injection of 8-Cl-cAMP in doses of 10,80 and 150 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP. These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of BUON", title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes", volume = "14", number = "1", pages = "71-77", url = "https://hdl.handle.net/21.15107/rcub_veterinar_614" }
Bajić, V., Stanimirović, Z., Stevanović, J., Spremo-Potparević, B., Zivković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77. https://hdl.handle.net/21.15107/rcub_veterinar_614
Bajić V, Stanimirović Z, Stevanović J, Spremo-Potparević B, Zivković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77. https://hdl.handle.net/21.15107/rcub_veterinar_614 .
Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Spremo-Potparević, Biljana, Zivković, L., Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77, https://hdl.handle.net/21.15107/rcub_veterinar_614 .