Expression of α-SMA, desmin and vimentin in canine liver with fibrosis
Ekspresija α-SMA, dezmina i vimentina u jetri pasa sa fibrozom
2009
Autori
Knežević, MilijanaGledić, Dušan
Kukolj, Vladimir
Knežević, Đ.
Jovanović, Milan
Božić, Tatjana
Aleksić-Kovačević, Sanja
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this work, 36 canine livers with fibrosis were examined. Based on the degree of fibrosis, the livers were classified into three groups: 1- mild portal fibrosis; 2- moderate portal fibrosis with mild periportal and septal fibrosis; 3- severe portal fibrosis with marked periportal and septal fibrosis or cirrhosis. Normal liver sections obtained from five dogs with no evidence of infectious, neoplastic, or cardiovascular diseases were used as controls. The degree of fibrosis was evaluated on liver sections stained according to Masson's trichrome method. It was proved using an immunohistochemical method that HSC and periductal myofibroblasts are immunoreactive to desmin, vimentin and α-SMA. Desmin, vimentin and α-SMA positive cells in the portal triad were significantly more numerous in dogs with fibrosis of the third degree than in the controls or all other examined livers. The density of α- SMA, vimentin and desmin-positive cells in the portal triad was in correlation with the degree ...of fibrosis, which cannot be said for HSC. These results suggest that portal myofibroblasts have a significant role in the process of liver fibrosis in dogs.
U ovom radu su prikazani rezultati ispitivanja 36 uzoraka tkiva jetre sa znacima fibroze, poreklom od pasa. Na osnovu stepena fibroze, uzorci su klasifikovani u tri grupe: 1 - blaga portalna fibroza; 2 - umerena portalna fibroza sa blagom periportalnom i septalnom fibrozom; 3 - teška portalna fibroza sa izraženom periportalnom i septalnom fibrozom ili cirozom. Kao kontrola su korišćeni uzorci tkiva jetre dobijeni od pet pasa bez znakova infekcije, neoplazije i kardiovaskularnih bolesti. Evaluacija stepena fibroze je urađena na isečcima jetre bojenim po Masson trihromnoj metodi. Imunohistohemijskom metodom je pokazano da su HSC i periduktalni miofibroblasti imunoreaktivni na dezmin, vimentin i α-SMA. Dezmin, vimentin i α-SMA, pozitivne ćelije u portalnoj trijadi bile su značajno brojnije kod pasa sa fibrozom trećeg stepena u odnosu na kontrolu i na sve druge ispitivane uzorke tkiva jetre. Gustina α-SMA, vimentin i dezmin pozitivnih ćelija u portalnoj trijadi je bila u korelaciji sa step...enom fibroze, što se ne može reći i za HSC. Ovi rezultati sugerišu da portalni miofibroblasti imaju značajnu ulogu u procesu fibroze jetre kod pasa.
Ključne reči:
dogs / HSC / immunohistochemistry / liver fibrosis / myofibroblastsIzvor:
Acta Veterinaria-Beograd, 2009, 59, 4, 361-370Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Difuzni sistem zvezdastih (stellata) ćelija i njihova uloga u oštećenju tkiva, reparaciji i neoplastičnim procesima kod sisara (RS-MESTD-MPN2006-2010-156010)
DOI: 10.2298/AVB0904361K
ISSN: 0567-8315
WoS: 000270452700004
Scopus: 2-s2.0-70349938041
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Knežević, Milijana AU - Gledić, Dušan AU - Kukolj, Vladimir AU - Knežević, Đ. AU - Jovanović, Milan AU - Božić, Tatjana AU - Aleksić-Kovačević, Sanja PY - 2009 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/653 AB - In this work, 36 canine livers with fibrosis were examined. Based on the degree of fibrosis, the livers were classified into three groups: 1- mild portal fibrosis; 2- moderate portal fibrosis with mild periportal and septal fibrosis; 3- severe portal fibrosis with marked periportal and septal fibrosis or cirrhosis. Normal liver sections obtained from five dogs with no evidence of infectious, neoplastic, or cardiovascular diseases were used as controls. The degree of fibrosis was evaluated on liver sections stained according to Masson's trichrome method. It was proved using an immunohistochemical method that HSC and periductal myofibroblasts are immunoreactive to desmin, vimentin and α-SMA. Desmin, vimentin and α-SMA positive cells in the portal triad were significantly more numerous in dogs with fibrosis of the third degree than in the controls or all other examined livers. The density of α- SMA, vimentin and desmin-positive cells in the portal triad was in correlation with the degree of fibrosis, which cannot be said for HSC. These results suggest that portal myofibroblasts have a significant role in the process of liver fibrosis in dogs. AB - U ovom radu su prikazani rezultati ispitivanja 36 uzoraka tkiva jetre sa znacima fibroze, poreklom od pasa. Na osnovu stepena fibroze, uzorci su klasifikovani u tri grupe: 1 - blaga portalna fibroza; 2 - umerena portalna fibroza sa blagom periportalnom i septalnom fibrozom; 3 - teška portalna fibroza sa izraženom periportalnom i septalnom fibrozom ili cirozom. Kao kontrola su korišćeni uzorci tkiva jetre dobijeni od pet pasa bez znakova infekcije, neoplazije i kardiovaskularnih bolesti. Evaluacija stepena fibroze je urađena na isečcima jetre bojenim po Masson trihromnoj metodi. Imunohistohemijskom metodom je pokazano da su HSC i periduktalni miofibroblasti imunoreaktivni na dezmin, vimentin i α-SMA. Dezmin, vimentin i α-SMA, pozitivne ćelije u portalnoj trijadi bile su značajno brojnije kod pasa sa fibrozom trećeg stepena u odnosu na kontrolu i na sve druge ispitivane uzorke tkiva jetre. Gustina α-SMA, vimentin i dezmin pozitivnih ćelija u portalnoj trijadi je bila u korelaciji sa stepenom fibroze, što se ne može reći i za HSC. Ovi rezultati sugerišu da portalni miofibroblasti imaju značajnu ulogu u procesu fibroze jetre kod pasa. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Expression of α-SMA, desmin and vimentin in canine liver with fibrosis T1 - Ekspresija α-SMA, dezmina i vimentina u jetri pasa sa fibrozom VL - 59 IS - 4 SP - 361 EP - 370 DO - 10.2298/AVB0904361K ER -
@article{ author = "Knežević, Milijana and Gledić, Dušan and Kukolj, Vladimir and Knežević, Đ. and Jovanović, Milan and Božić, Tatjana and Aleksić-Kovačević, Sanja", year = "2009", abstract = "In this work, 36 canine livers with fibrosis were examined. Based on the degree of fibrosis, the livers were classified into three groups: 1- mild portal fibrosis; 2- moderate portal fibrosis with mild periportal and septal fibrosis; 3- severe portal fibrosis with marked periportal and septal fibrosis or cirrhosis. Normal liver sections obtained from five dogs with no evidence of infectious, neoplastic, or cardiovascular diseases were used as controls. The degree of fibrosis was evaluated on liver sections stained according to Masson's trichrome method. It was proved using an immunohistochemical method that HSC and periductal myofibroblasts are immunoreactive to desmin, vimentin and α-SMA. Desmin, vimentin and α-SMA positive cells in the portal triad were significantly more numerous in dogs with fibrosis of the third degree than in the controls or all other examined livers. The density of α- SMA, vimentin and desmin-positive cells in the portal triad was in correlation with the degree of fibrosis, which cannot be said for HSC. These results suggest that portal myofibroblasts have a significant role in the process of liver fibrosis in dogs., U ovom radu su prikazani rezultati ispitivanja 36 uzoraka tkiva jetre sa znacima fibroze, poreklom od pasa. Na osnovu stepena fibroze, uzorci su klasifikovani u tri grupe: 1 - blaga portalna fibroza; 2 - umerena portalna fibroza sa blagom periportalnom i septalnom fibrozom; 3 - teška portalna fibroza sa izraženom periportalnom i septalnom fibrozom ili cirozom. Kao kontrola su korišćeni uzorci tkiva jetre dobijeni od pet pasa bez znakova infekcije, neoplazije i kardiovaskularnih bolesti. Evaluacija stepena fibroze je urađena na isečcima jetre bojenim po Masson trihromnoj metodi. Imunohistohemijskom metodom je pokazano da su HSC i periduktalni miofibroblasti imunoreaktivni na dezmin, vimentin i α-SMA. Dezmin, vimentin i α-SMA, pozitivne ćelije u portalnoj trijadi bile su značajno brojnije kod pasa sa fibrozom trećeg stepena u odnosu na kontrolu i na sve druge ispitivane uzorke tkiva jetre. Gustina α-SMA, vimentin i dezmin pozitivnih ćelija u portalnoj trijadi je bila u korelaciji sa stepenom fibroze, što se ne može reći i za HSC. Ovi rezultati sugerišu da portalni miofibroblasti imaju značajnu ulogu u procesu fibroze jetre kod pasa.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Expression of α-SMA, desmin and vimentin in canine liver with fibrosis, Ekspresija α-SMA, dezmina i vimentina u jetri pasa sa fibrozom", volume = "59", number = "4", pages = "361-370", doi = "10.2298/AVB0904361K" }
Knežević, M., Gledić, D., Kukolj, V., Knežević, Đ., Jovanović, M., Božić, T.,& Aleksić-Kovačević, S.. (2009). Expression of α-SMA, desmin and vimentin in canine liver with fibrosis. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 361-370. https://doi.org/10.2298/AVB0904361K
Knežević M, Gledić D, Kukolj V, Knežević Đ, Jovanović M, Božić T, Aleksić-Kovačević S. Expression of α-SMA, desmin and vimentin in canine liver with fibrosis. in Acta Veterinaria-Beograd. 2009;59(4):361-370. doi:10.2298/AVB0904361K .
Knežević, Milijana, Gledić, Dušan, Kukolj, Vladimir, Knežević, Đ., Jovanović, Milan, Božić, Tatjana, Aleksić-Kovačević, Sanja, "Expression of α-SMA, desmin and vimentin in canine liver with fibrosis" in Acta Veterinaria-Beograd, 59, no. 4 (2009):361-370, https://doi.org/10.2298/AVB0904361K . .