Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A
Status serumskih proteina brojlera hranjenih obrocima sa rastućim koncentracijama ohratoksina A
Abstract
The 42-day long study was performed on a total number of 48 Hybro broilers divided into four groups. After the pre-experimental period of 14 days, 3 experimental groups of broilers (n=12) were formed and fed diets that contained 0.5, 1.0 and 1.5 mg/kg ochratoxin A (OTA) during the next seven consecutive days. In the same period, the control group of broilers was fed a diet with no toxin added. After the period of toxin addition, blood samples were taken from 6 animals in each group. The remaining animals (n=6) from the control and experimental groups were fed diets without OTA until the 42nd day of the study, when the blood samples were taken again. The total level of blood serum proteins was affected by treatment with different doses of OTA, but a significant and dose dependent increase of albumins together with a decrease of γ-globulin fraction was established. A/G ratio (Albumine/Globuline) suggested that the globulins were the dominant protein fraction in the blood serum samples ob...tained from all the broilers included in this study. The concentrations of α- and β- globulin in the serum were within physiological limits, but the concentration of γ-globulins significantly decreased. It can be concluded that the increasing dietary OTA levels (0.5, 1.0 and 1.5 mg/kg) had dose-dependent cumulative effect on blood serum proteins status in broilers, and the effect lasts even after the withdrawal of OTA from the feed.
Ispitivanje je trajalo 42 dan na ukupno 48 brojlera podeljenih u četiri jednake grupe. Preeksperimentalni period trajao je 14 dana, nakon čega su životinje tri ogledne grupe tokom sedam dana hranjene obrocma koji su sadržali 0,5, 1,0 i 1,5 mg ohratoksina A (OTA) u 1kg hrane. Brojleri kontrolne grupe u toku eksperimentalnog perioda hranjeni su hranom bez dodatka toksina. Posle sedam dana tretmana uzimani su uzorci krvi od po šest životinja iz svake grupe (kontrolne i eksperimentalnih). Preostale životinje hranjene su obrocima bez toksina do 42. dana ogleda, nakon čega je krv uzorkovana i od njih. Koncentracija ukupnih proteina plazme bila je promenjena u zavisnosti od koncentracije OTA koji su brojleri dobijali hranom. Takođe je zabeleženo značajno i dozno-zavisno povećanje koncentracije albumina sa snižavanjem koncentracije gama-globulinske frakcije u serumu. Albulin/Globulin odnos (A/G odnos) ukazuje da su globulini bili dominantna frakcija proteina kod svih brojlera u ispitivanju. Ko...ncentracija alfa i beta-globulina u serumu brojlera kretala se u fiziološkim granicama dok je koncentracija gamaglobulina bila značajno snižena. Može se zaključiti da rastuće koncentracije OTA u hrani brojlera ispoljavaju dozno-zavistan kumulativni efekt na status serumskih proteina. Ovaj efekat ostaje i posle prestanka dodavanja toksina u hranu.
Keywords:
A/G ratio / broiler / ochratoxin A / poultry / proteinsSource:
Acta Veterinaria-Beograd, 2013, 63, 1, 77-88Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Development of herbal medicines and biocides on the basis of Carvacrol, Thymol and Cinnamaldehyde for use in veterinary medicine, animal husbandry and food production without harmful residues (RS-MESTD-Technological Development (TD or TR)-31087)
DOI: 10.2298/AVB1301077N
ISSN: 0567-8315
WoS: 000316523900007
Scopus: 2-s2.0-84879101082
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Fakultet veterinarske medicineTY - JOUR AU - Nedeljković-Trailović, Jelena AU - Trailović, Saša AU - Dimitrijević, Mirjana AU - Ilić, Vojislav PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1024 AB - The 42-day long study was performed on a total number of 48 Hybro broilers divided into four groups. After the pre-experimental period of 14 days, 3 experimental groups of broilers (n=12) were formed and fed diets that contained 0.5, 1.0 and 1.5 mg/kg ochratoxin A (OTA) during the next seven consecutive days. In the same period, the control group of broilers was fed a diet with no toxin added. After the period of toxin addition, blood samples were taken from 6 animals in each group. The remaining animals (n=6) from the control and experimental groups were fed diets without OTA until the 42nd day of the study, when the blood samples were taken again. The total level of blood serum proteins was affected by treatment with different doses of OTA, but a significant and dose dependent increase of albumins together with a decrease of γ-globulin fraction was established. A/G ratio (Albumine/Globuline) suggested that the globulins were the dominant protein fraction in the blood serum samples obtained from all the broilers included in this study. The concentrations of α- and β- globulin in the serum were within physiological limits, but the concentration of γ-globulins significantly decreased. It can be concluded that the increasing dietary OTA levels (0.5, 1.0 and 1.5 mg/kg) had dose-dependent cumulative effect on blood serum proteins status in broilers, and the effect lasts even after the withdrawal of OTA from the feed. AB - Ispitivanje je trajalo 42 dan na ukupno 48 brojlera podeljenih u četiri jednake grupe. Preeksperimentalni period trajao je 14 dana, nakon čega su životinje tri ogledne grupe tokom sedam dana hranjene obrocma koji su sadržali 0,5, 1,0 i 1,5 mg ohratoksina A (OTA) u 1kg hrane. Brojleri kontrolne grupe u toku eksperimentalnog perioda hranjeni su hranom bez dodatka toksina. Posle sedam dana tretmana uzimani su uzorci krvi od po šest životinja iz svake grupe (kontrolne i eksperimentalnih). Preostale životinje hranjene su obrocima bez toksina do 42. dana ogleda, nakon čega je krv uzorkovana i od njih. Koncentracija ukupnih proteina plazme bila je promenjena u zavisnosti od koncentracije OTA koji su brojleri dobijali hranom. Takođe je zabeleženo značajno i dozno-zavisno povećanje koncentracije albumina sa snižavanjem koncentracije gama-globulinske frakcije u serumu. Albulin/Globulin odnos (A/G odnos) ukazuje da su globulini bili dominantna frakcija proteina kod svih brojlera u ispitivanju. Koncentracija alfa i beta-globulina u serumu brojlera kretala se u fiziološkim granicama dok je koncentracija gamaglobulina bila značajno snižena. Može se zaključiti da rastuće koncentracije OTA u hrani brojlera ispoljavaju dozno-zavistan kumulativni efekt na status serumskih proteina. Ovaj efekat ostaje i posle prestanka dodavanja toksina u hranu. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A T1 - Status serumskih proteina brojlera hranjenih obrocima sa rastućim koncentracijama ohratoksina A VL - 63 IS - 1 SP - 77 EP - 88 DO - 10.2298/AVB1301077N ER -
@article{ author = "Nedeljković-Trailović, Jelena and Trailović, Saša and Dimitrijević, Mirjana and Ilić, Vojislav", year = "2013", abstract = "The 42-day long study was performed on a total number of 48 Hybro broilers divided into four groups. After the pre-experimental period of 14 days, 3 experimental groups of broilers (n=12) were formed and fed diets that contained 0.5, 1.0 and 1.5 mg/kg ochratoxin A (OTA) during the next seven consecutive days. In the same period, the control group of broilers was fed a diet with no toxin added. After the period of toxin addition, blood samples were taken from 6 animals in each group. The remaining animals (n=6) from the control and experimental groups were fed diets without OTA until the 42nd day of the study, when the blood samples were taken again. The total level of blood serum proteins was affected by treatment with different doses of OTA, but a significant and dose dependent increase of albumins together with a decrease of γ-globulin fraction was established. A/G ratio (Albumine/Globuline) suggested that the globulins were the dominant protein fraction in the blood serum samples obtained from all the broilers included in this study. The concentrations of α- and β- globulin in the serum were within physiological limits, but the concentration of γ-globulins significantly decreased. It can be concluded that the increasing dietary OTA levels (0.5, 1.0 and 1.5 mg/kg) had dose-dependent cumulative effect on blood serum proteins status in broilers, and the effect lasts even after the withdrawal of OTA from the feed., Ispitivanje je trajalo 42 dan na ukupno 48 brojlera podeljenih u četiri jednake grupe. Preeksperimentalni period trajao je 14 dana, nakon čega su životinje tri ogledne grupe tokom sedam dana hranjene obrocma koji su sadržali 0,5, 1,0 i 1,5 mg ohratoksina A (OTA) u 1kg hrane. Brojleri kontrolne grupe u toku eksperimentalnog perioda hranjeni su hranom bez dodatka toksina. Posle sedam dana tretmana uzimani su uzorci krvi od po šest životinja iz svake grupe (kontrolne i eksperimentalnih). Preostale životinje hranjene su obrocima bez toksina do 42. dana ogleda, nakon čega je krv uzorkovana i od njih. Koncentracija ukupnih proteina plazme bila je promenjena u zavisnosti od koncentracije OTA koji su brojleri dobijali hranom. Takođe je zabeleženo značajno i dozno-zavisno povećanje koncentracije albumina sa snižavanjem koncentracije gama-globulinske frakcije u serumu. Albulin/Globulin odnos (A/G odnos) ukazuje da su globulini bili dominantna frakcija proteina kod svih brojlera u ispitivanju. Koncentracija alfa i beta-globulina u serumu brojlera kretala se u fiziološkim granicama dok je koncentracija gamaglobulina bila značajno snižena. Može se zaključiti da rastuće koncentracije OTA u hrani brojlera ispoljavaju dozno-zavistan kumulativni efekt na status serumskih proteina. Ovaj efekat ostaje i posle prestanka dodavanja toksina u hranu.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A, Status serumskih proteina brojlera hranjenih obrocima sa rastućim koncentracijama ohratoksina A", volume = "63", number = "1", pages = "77-88", doi = "10.2298/AVB1301077N" }
Nedeljković-Trailović, J., Trailović, S., Dimitrijević, M.,& Ilić, V.. (2013). Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1), 77-88. https://doi.org/10.2298/AVB1301077N
Nedeljković-Trailović J, Trailović S, Dimitrijević M, Ilić V. Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A. in Acta Veterinaria-Beograd. 2013;63(1):77-88. doi:10.2298/AVB1301077N .
Nedeljković-Trailović, Jelena, Trailović, Saša, Dimitrijević, Mirjana, Ilić, Vojislav, "Blood serum protein status in broilers fed with increasing concentrations of ochratoxin A" in Acta Veterinaria-Beograd, 63, no. 1 (2013):77-88, https://doi.org/10.2298/AVB1301077N . .