Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens
Апстракт
The indirect immunoperoxidase method (IP) with polyclonal antibodies was used for detecting infectious bursal disease (IBD) virus antigen in the bursa of chickens experimentally infected with a highly virulent vi,vs designated as KA. Detection of IBD virus by the IP method on methanol fixed paraffin embedded tissue samples is possible ten days after infection (PI). Three to seven days PI an intensive expression of IBDV antigen can be observed on lymphocytes in the cortex and medulla of bursal follicles, as well as on macrophages in the follicles and interstitium. Ten days PI the immunohistochemical reaction is very discreet, but IBD virus detection is still possible. The IP method, used in our work, is a sensitive and specific way of demonstrating IBDV in the bursa of Fabricius of experimentally infected chickens.
Кључне речи:
chicken / infectious bursal disease / virus / immunohistochemistryИзвор:
Acta Veterinaria-Beograd, 1999, 49, 1, 13-19Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Aleksić-Kovačević, Sanja AU - Gagić, M AU - Lazić, S AU - Kovačević, M PY - 1999 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/108 AB - The indirect immunoperoxidase method (IP) with polyclonal antibodies was used for detecting infectious bursal disease (IBD) virus antigen in the bursa of chickens experimentally infected with a highly virulent vi,vs designated as KA. Detection of IBD virus by the IP method on methanol fixed paraffin embedded tissue samples is possible ten days after infection (PI). Three to seven days PI an intensive expression of IBDV antigen can be observed on lymphocytes in the cortex and medulla of bursal follicles, as well as on macrophages in the follicles and interstitium. Ten days PI the immunohistochemical reaction is very discreet, but IBD virus detection is still possible. The IP method, used in our work, is a sensitive and specific way of demonstrating IBDV in the bursa of Fabricius of experimentally infected chickens. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens VL - 49 IS - 1 SP - 13 EP - 19 UR - https://hdl.handle.net/21.15107/rcub_veterinar_108 ER -
@article{ author = "Aleksić-Kovačević, Sanja and Gagić, M and Lazić, S and Kovačević, M", year = "1999", abstract = "The indirect immunoperoxidase method (IP) with polyclonal antibodies was used for detecting infectious bursal disease (IBD) virus antigen in the bursa of chickens experimentally infected with a highly virulent vi,vs designated as KA. Detection of IBD virus by the IP method on methanol fixed paraffin embedded tissue samples is possible ten days after infection (PI). Three to seven days PI an intensive expression of IBDV antigen can be observed on lymphocytes in the cortex and medulla of bursal follicles, as well as on macrophages in the follicles and interstitium. Ten days PI the immunohistochemical reaction is very discreet, but IBD virus detection is still possible. The IP method, used in our work, is a sensitive and specific way of demonstrating IBDV in the bursa of Fabricius of experimentally infected chickens.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens", volume = "49", number = "1", pages = "13-19", url = "https://hdl.handle.net/21.15107/rcub_veterinar_108" }
Aleksić-Kovačević, S., Gagić, M., Lazić, S.,& Kovačević, M.. (1999). Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 49(1), 13-19. https://hdl.handle.net/21.15107/rcub_veterinar_108
Aleksić-Kovačević S, Gagić M, Lazić S, Kovačević M. Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens. in Acta Veterinaria-Beograd. 1999;49(1):13-19. https://hdl.handle.net/21.15107/rcub_veterinar_108 .
Aleksić-Kovačević, Sanja, Gagić, M, Lazić, S, Kovačević, M, "Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens" in Acta Veterinaria-Beograd, 49, no. 1 (1999):13-19, https://hdl.handle.net/21.15107/rcub_veterinar_108 .