The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle
Abstract
Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.
Keywords:
Bovine parainfluenza 3 virus / RT-PCR / direct sequencingSource:
Archives of Biological Sciences, 2014, 66, 2, 491-496Publisher:
- Srpsko biološko društvo, Beograd
Funding / projects:
- Development and application of molecular methods based on polymerase chain reaction (PCR) in rapid and direct identification of Newcastle disease virus strains and examination of immunogenicity of subunit vaccine prepared from their antigens (RS-MESTD-Technological Development (TD or TR)-31008)
- Importance of viral genetic variability in human diseases (RS-MESTD-Basic Research (BR or ON)-175073)
DOI: 10.2298/ABS1402491V
ISSN: 0354-4664
WoS: 000342265800006
Scopus: 2-s2.0-84903952082
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Veljović, Ljubiša AU - Knežević, Aleksandra AU - Milić, Nenad AU - Nišavić, Jakov PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1092 AB - Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia. PB - Srpsko biološko društvo, Beograd T2 - Archives of Biological Sciences T1 - The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle VL - 66 IS - 2 SP - 491 EP - 496 DO - 10.2298/ABS1402491V ER -
@article{ author = "Veljović, Ljubiša and Knežević, Aleksandra and Milić, Nenad and Nišavić, Jakov", year = "2014", abstract = "Bovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.", publisher = "Srpsko biološko društvo, Beograd", journal = "Archives of Biological Sciences", title = "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle", volume = "66", number = "2", pages = "491-496", doi = "10.2298/ABS1402491V" }
Veljović, L., Knežević, A., Milić, N.,& Nišavić, J.. (2014). The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences Srpsko biološko društvo, Beograd., 66(2), 491-496. https://doi.org/10.2298/ABS1402491V
Veljović L, Knežević A, Milić N, Nišavić J. The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle. in Archives of Biological Sciences. 2014;66(2):491-496. doi:10.2298/ABS1402491V .
Veljović, Ljubiša, Knežević, Aleksandra, Milić, Nenad, Nišavić, Jakov, "The application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattle" in Archives of Biological Sciences, 66, no. 2 (2014):491-496, https://doi.org/10.2298/ABS1402491V . .