Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle
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2015
Authors
Stevanov-Pavlović, MarijaDimitrijević, Vladimir
Marić, Sasa
Radović, Dejan
Stevanović, Jevrosima
Stanimirović, Zoran
Article (Published version)
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Show full item recordAbstract
This study is focused on evaluating 12 microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics (ISAG) for paternity testing in Busha cattle in Serbia. A herd of 47 heads of Busha cattle were sampled for blood. The number of alleles ranged from 6 (ETH10) to 16 (TGLA122), with a mean value of 9.5. Total number of alleles at all 12 analyzed loci was 114. PIC (Polymorphism Information Content) values ranged from 0.513 (in BM1818) to 0.905 (TGLA53). Power of exclusion (PE) for single markers ranged from 0.228 (BM1818) to 0.607 (BM2113) and the power of discrimination (PD) from 0.75 (BM1818) to 0.96 (TGLA227). The combined power of exclusion and discrimination was very high (0.999 and 0.995 respectively) when all 12 markers were used in combination. Cervus software performed 96% successful paternity assignments. These results show that the 12 markers set recommended by I...SAG can be used with high confidence for forensic purposes in Busha cattle.
Keywords:
microsatellite / paternity testing / busa / bushaSource:
Slovenian Veterinary Research, 2015, 52, 3, 133-139Publisher:
- Univ Ljubljana, Veterinary Faculty, Ljubljana
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
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Fakultet veterinarske medicineTY - JOUR AU - Stevanov-Pavlović, Marija AU - Dimitrijević, Vladimir AU - Marić, Sasa AU - Radović, Dejan AU - Stevanović, Jevrosima AU - Stanimirović, Zoran PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1277 AB - This study is focused on evaluating 12 microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics (ISAG) for paternity testing in Busha cattle in Serbia. A herd of 47 heads of Busha cattle were sampled for blood. The number of alleles ranged from 6 (ETH10) to 16 (TGLA122), with a mean value of 9.5. Total number of alleles at all 12 analyzed loci was 114. PIC (Polymorphism Information Content) values ranged from 0.513 (in BM1818) to 0.905 (TGLA53). Power of exclusion (PE) for single markers ranged from 0.228 (BM1818) to 0.607 (BM2113) and the power of discrimination (PD) from 0.75 (BM1818) to 0.96 (TGLA227). The combined power of exclusion and discrimination was very high (0.999 and 0.995 respectively) when all 12 markers were used in combination. Cervus software performed 96% successful paternity assignments. These results show that the 12 markers set recommended by ISAG can be used with high confidence for forensic purposes in Busha cattle. PB - Univ Ljubljana, Veterinary Faculty, Ljubljana T2 - Slovenian Veterinary Research T1 - Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle VL - 52 IS - 3 SP - 133 EP - 139 UR - https://hdl.handle.net/21.15107/rcub_veterinar_1277 ER -
@article{ author = "Stevanov-Pavlović, Marija and Dimitrijević, Vladimir and Marić, Sasa and Radović, Dejan and Stevanović, Jevrosima and Stanimirović, Zoran", year = "2015", abstract = "This study is focused on evaluating 12 microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics (ISAG) for paternity testing in Busha cattle in Serbia. A herd of 47 heads of Busha cattle were sampled for blood. The number of alleles ranged from 6 (ETH10) to 16 (TGLA122), with a mean value of 9.5. Total number of alleles at all 12 analyzed loci was 114. PIC (Polymorphism Information Content) values ranged from 0.513 (in BM1818) to 0.905 (TGLA53). Power of exclusion (PE) for single markers ranged from 0.228 (BM1818) to 0.607 (BM2113) and the power of discrimination (PD) from 0.75 (BM1818) to 0.96 (TGLA227). The combined power of exclusion and discrimination was very high (0.999 and 0.995 respectively) when all 12 markers were used in combination. Cervus software performed 96% successful paternity assignments. These results show that the 12 markers set recommended by ISAG can be used with high confidence for forensic purposes in Busha cattle.", publisher = "Univ Ljubljana, Veterinary Faculty, Ljubljana", journal = "Slovenian Veterinary Research", title = "Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle", volume = "52", number = "3", pages = "133-139", url = "https://hdl.handle.net/21.15107/rcub_veterinar_1277" }
Stevanov-Pavlović, M., Dimitrijević, V., Marić, S., Radović, D., Stevanović, J.,& Stanimirović, Z.. (2015). Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle. in Slovenian Veterinary Research Univ Ljubljana, Veterinary Faculty, Ljubljana., 52(3), 133-139. https://hdl.handle.net/21.15107/rcub_veterinar_1277
Stevanov-Pavlović M, Dimitrijević V, Marić S, Radović D, Stevanović J, Stanimirović Z. Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle. in Slovenian Veterinary Research. 2015;52(3):133-139. https://hdl.handle.net/21.15107/rcub_veterinar_1277 .
Stevanov-Pavlović, Marija, Dimitrijević, Vladimir, Marić, Sasa, Radović, Dejan, Stevanović, Jevrosima, Stanimirović, Zoran, "Applicability assessment of a standardized microsatellite marker set in endangered Busha cattle" in Slovenian Veterinary Research, 52, no. 3 (2015):133-139, https://hdl.handle.net/21.15107/rcub_veterinar_1277 .