In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines
In vivo ispitivanje enzimske aktivnosti nekih Ru(II) jedinjenja sa N-alkilfenotiazinima
Abstract
The purpose of the present study was to investigate and compare the effects of two ruthenium complexes with trifluoperazine on acethylcholinesterase enzyme activity and lactate dehydrogenase levels in vivo under physiological conditions in rats blood. Complexes 1 and 2 showed positive effects on acethylcholinesterase at all doses and did not disturb its normal activity. Total LDH activity was inhibited in the presence of both complexes, but Ru(II) complexes showed different effects on the activity of LDH isoenzymes. The activities of LDH1 and LDH2 isoenzymes were decreased in all applied doses of the complex 2, while the activity of LDH2 reduced using complex 1 in the same doses. Results of the present study suggest the neuro- and cardio protective potential of oral administration of complexes 1 and 2, as non-toxic compounds under physiological conditions. These protective effects are the result of their potent antioxidant activity.
Cilj ovog rada je da se ispitaju i uporede efekti dva kompleksa rutenijuma sa trifluoperazinom na aktivnost enzima acetilholinesteraze i laktat-dehidrogenase in vivo pod fiziološkim uslovima u krvi pacova. Kompleksi 1 i 2 pokazali su pozitivan efekat na aktivnost acetiholinesteraze u svim primenjenim dozama. Ukupna aktivnost LDH je inhibirana u prisustvu oba kompleksa, ali kompleksi Ru(II) pokazuju različite rezultate na izoenzimske oblike ovog enzima. Aktivnosti izoenzima LDH1 i LDH2 su smanjene u svim primenjenim dozama kompleksa 2, dok kompleks 1 smanjuje aktivnost samo izoenzima LDH2 u tim istim koncentracijama. Rezultati prikazanog istraživanja ukazuju na neuro - i kardio zaštitni potencijal oralne primene kompleksa 1 i 2, kao netoksičnih jedinjenja pod fiziološkim uslovima, indukovano preko njihovog snažnog antioksidativnog efekta.
Keywords:
Ruthenium complexes / N-alkylphenothiazines / acethylcholinesterase / lactate dehydrogenaseSource:
Acta Veterinaria-Beograd, 2016, 66, 4, 497-508Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Design, synthesis, characterization and assessment of practical applications of coordination and organometallic compounds (RS-MESTD-Basic Research (BR or ON)-172014)
DOI: 10.1515/acve-2016-0043
ISSN: 0567-8315
WoS: 000392931700007
Scopus: 2-s2.0-85013374578
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Fakultet veterinarske medicineTY - JOUR AU - Krstić, Milena AU - Borozan, Sunčica AU - Sovilj, Sofija P. AU - Grgurić-Šipka, Sanja AU - Poljarević, Jelena PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1353 AB - The purpose of the present study was to investigate and compare the effects of two ruthenium complexes with trifluoperazine on acethylcholinesterase enzyme activity and lactate dehydrogenase levels in vivo under physiological conditions in rats blood. Complexes 1 and 2 showed positive effects on acethylcholinesterase at all doses and did not disturb its normal activity. Total LDH activity was inhibited in the presence of both complexes, but Ru(II) complexes showed different effects on the activity of LDH isoenzymes. The activities of LDH1 and LDH2 isoenzymes were decreased in all applied doses of the complex 2, while the activity of LDH2 reduced using complex 1 in the same doses. Results of the present study suggest the neuro- and cardio protective potential of oral administration of complexes 1 and 2, as non-toxic compounds under physiological conditions. These protective effects are the result of their potent antioxidant activity. AB - Cilj ovog rada je da se ispitaju i uporede efekti dva kompleksa rutenijuma sa trifluoperazinom na aktivnost enzima acetilholinesteraze i laktat-dehidrogenase in vivo pod fiziološkim uslovima u krvi pacova. Kompleksi 1 i 2 pokazali su pozitivan efekat na aktivnost acetiholinesteraze u svim primenjenim dozama. Ukupna aktivnost LDH je inhibirana u prisustvu oba kompleksa, ali kompleksi Ru(II) pokazuju različite rezultate na izoenzimske oblike ovog enzima. Aktivnosti izoenzima LDH1 i LDH2 su smanjene u svim primenjenim dozama kompleksa 2, dok kompleks 1 smanjuje aktivnost samo izoenzima LDH2 u tim istim koncentracijama. Rezultati prikazanog istraživanja ukazuju na neuro - i kardio zaštitni potencijal oralne primene kompleksa 1 i 2, kao netoksičnih jedinjenja pod fiziološkim uslovima, indukovano preko njihovog snažnog antioksidativnog efekta. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines T1 - In vivo ispitivanje enzimske aktivnosti nekih Ru(II) jedinjenja sa N-alkilfenotiazinima VL - 66 IS - 4 SP - 497 EP - 508 DO - 10.1515/acve-2016-0043 ER -
@article{ author = "Krstić, Milena and Borozan, Sunčica and Sovilj, Sofija P. and Grgurić-Šipka, Sanja and Poljarević, Jelena", year = "2016", abstract = "The purpose of the present study was to investigate and compare the effects of two ruthenium complexes with trifluoperazine on acethylcholinesterase enzyme activity and lactate dehydrogenase levels in vivo under physiological conditions in rats blood. Complexes 1 and 2 showed positive effects on acethylcholinesterase at all doses and did not disturb its normal activity. Total LDH activity was inhibited in the presence of both complexes, but Ru(II) complexes showed different effects on the activity of LDH isoenzymes. The activities of LDH1 and LDH2 isoenzymes were decreased in all applied doses of the complex 2, while the activity of LDH2 reduced using complex 1 in the same doses. Results of the present study suggest the neuro- and cardio protective potential of oral administration of complexes 1 and 2, as non-toxic compounds under physiological conditions. These protective effects are the result of their potent antioxidant activity., Cilj ovog rada je da se ispitaju i uporede efekti dva kompleksa rutenijuma sa trifluoperazinom na aktivnost enzima acetilholinesteraze i laktat-dehidrogenase in vivo pod fiziološkim uslovima u krvi pacova. Kompleksi 1 i 2 pokazali su pozitivan efekat na aktivnost acetiholinesteraze u svim primenjenim dozama. Ukupna aktivnost LDH je inhibirana u prisustvu oba kompleksa, ali kompleksi Ru(II) pokazuju različite rezultate na izoenzimske oblike ovog enzima. Aktivnosti izoenzima LDH1 i LDH2 su smanjene u svim primenjenim dozama kompleksa 2, dok kompleks 1 smanjuje aktivnost samo izoenzima LDH2 u tim istim koncentracijama. Rezultati prikazanog istraživanja ukazuju na neuro - i kardio zaštitni potencijal oralne primene kompleksa 1 i 2, kao netoksičnih jedinjenja pod fiziološkim uslovima, indukovano preko njihovog snažnog antioksidativnog efekta.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines, In vivo ispitivanje enzimske aktivnosti nekih Ru(II) jedinjenja sa N-alkilfenotiazinima", volume = "66", number = "4", pages = "497-508", doi = "10.1515/acve-2016-0043" }
Krstić, M., Borozan, S., Sovilj, S. P., Grgurić-Šipka, S.,& Poljarević, J.. (2016). In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 497-508. https://doi.org/10.1515/acve-2016-0043
Krstić M, Borozan S, Sovilj SP, Grgurić-Šipka S, Poljarević J. In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines. in Acta Veterinaria-Beograd. 2016;66(4):497-508. doi:10.1515/acve-2016-0043 .
Krstić, Milena, Borozan, Sunčica, Sovilj, Sofija P., Grgurić-Šipka, Sanja, Poljarević, Jelena, "In vivo enzymes activities of some Ru(II) compounds with N-alkylphenothiazines" in Acta Veterinaria-Beograd, 66, no. 4 (2016):497-508, https://doi.org/10.1515/acve-2016-0043 . .