Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees
2016
Authors
Stevanović, JevrosimaSchwarz, Ryan S.
Vejnović, Branislav
Evans, Jay D.
Irwin, Rebecca E.
Glavinić, Uroš
Stanimirović, Zoran
Article (Published version)
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In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were validated for target specificity under single and mixed-species conditions as well as against the bumblebee trypanosomatid Crithidia bombi. Infections by Nosema apis and Nosema ceranae (Microsporidia) were also determined using PCR. Samples from 162 colonies (18 from each year) originating from 57 different localities were surveyed. L. passim was detected in every year with an overall frequency of 62.3% and annual frequencies ranging from 38.9% to 83.3%. This provides the earliest confirmed record to date for L. passim and the first report of this species in Serbia. N. ceranae was ubiquit...ous, occurring in every year and at 95.7% overall frequency, ranging annually from 83.3% to 100%. The majority of colonies (60.5%) were co-infected with L. passim and N. ceranae, but colony infections by each species were statistically independent of one another over the nine years. Although C. mellificae and N. apis have both been reported recently at low frequency in Europe, neither of these species was detected in Serbia. These results support the hypothesis that L. passim has predominated over C mellificae in A. mellifera during the past decade.
Keywords:
Crithidia / Lotmaria / Molecular diagnostics / Nosema / Pathogen surveySource:
Journal of Invertebrate Pathology, 2016, 139, 6-11Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- U.S. National Science Foundation - NSFNational Science Foundation (NSF) [1046153, 1415604]
DOI: 10.1016/j.jip.2016.07.001
ISSN: 0022-2011
PubMed: 27392956
WoS: 000382712500002
Scopus: 2-s2.0-84978066596
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Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Jevrosima AU - Schwarz, Ryan S. AU - Vejnović, Branislav AU - Evans, Jay D. AU - Irwin, Rebecca E. AU - Glavinić, Uroš AU - Stanimirović, Zoran PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1391 AB - In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were validated for target specificity under single and mixed-species conditions as well as against the bumblebee trypanosomatid Crithidia bombi. Infections by Nosema apis and Nosema ceranae (Microsporidia) were also determined using PCR. Samples from 162 colonies (18 from each year) originating from 57 different localities were surveyed. L. passim was detected in every year with an overall frequency of 62.3% and annual frequencies ranging from 38.9% to 83.3%. This provides the earliest confirmed record to date for L. passim and the first report of this species in Serbia. N. ceranae was ubiquitous, occurring in every year and at 95.7% overall frequency, ranging annually from 83.3% to 100%. The majority of colonies (60.5%) were co-infected with L. passim and N. ceranae, but colony infections by each species were statistically independent of one another over the nine years. Although C. mellificae and N. apis have both been reported recently at low frequency in Europe, neither of these species was detected in Serbia. These results support the hypothesis that L. passim has predominated over C mellificae in A. mellifera during the past decade. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Invertebrate Pathology T1 - Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees VL - 139 SP - 6 EP - 11 DO - 10.1016/j.jip.2016.07.001 ER -
@article{ author = "Stevanović, Jevrosima and Schwarz, Ryan S. and Vejnović, Branislav and Evans, Jay D. and Irwin, Rebecca E. and Glavinić, Uroš and Stanimirović, Zoran", year = "2016", abstract = "In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were validated for target specificity under single and mixed-species conditions as well as against the bumblebee trypanosomatid Crithidia bombi. Infections by Nosema apis and Nosema ceranae (Microsporidia) were also determined using PCR. Samples from 162 colonies (18 from each year) originating from 57 different localities were surveyed. L. passim was detected in every year with an overall frequency of 62.3% and annual frequencies ranging from 38.9% to 83.3%. This provides the earliest confirmed record to date for L. passim and the first report of this species in Serbia. N. ceranae was ubiquitous, occurring in every year and at 95.7% overall frequency, ranging annually from 83.3% to 100%. The majority of colonies (60.5%) were co-infected with L. passim and N. ceranae, but colony infections by each species were statistically independent of one another over the nine years. Although C. mellificae and N. apis have both been reported recently at low frequency in Europe, neither of these species was detected in Serbia. These results support the hypothesis that L. passim has predominated over C mellificae in A. mellifera during the past decade.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Invertebrate Pathology", title = "Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees", volume = "139", pages = "6-11", doi = "10.1016/j.jip.2016.07.001" }
Stevanović, J., Schwarz, R. S., Vejnović, B., Evans, J. D., Irwin, R. E., Glavinić, U.,& Stanimirović, Z.. (2016). Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees. in Journal of Invertebrate Pathology Academic Press Inc Elsevier Science, San Diego., 139, 6-11. https://doi.org/10.1016/j.jip.2016.07.001
Stevanović J, Schwarz RS, Vejnović B, Evans JD, Irwin RE, Glavinić U, Stanimirović Z. Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees. in Journal of Invertebrate Pathology. 2016;139:6-11. doi:10.1016/j.jip.2016.07.001 .
Stevanović, Jevrosima, Schwarz, Ryan S., Vejnović, Branislav, Evans, Jay D., Irwin, Rebecca E., Glavinić, Uroš, Stanimirović, Zoran, "Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees" in Journal of Invertebrate Pathology, 139 (2016):6-11, https://doi.org/10.1016/j.jip.2016.07.001 . .