Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods
Само за регистроване кориснике
2016
Аутори
Gajić, BojanStevanović, Jevrosima
Radulović, Željko
Kulišić, Zoran
Vejnović, Branislav
Glavinić, Uroš
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that thi...s is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.
Кључне речи:
Varroa destructor / Haplotype / mtDNA heteroplasmy / ARMS / PCR-RFLPИзвор:
Experimental and Applied Acarology, 2016, 70, 3, 287-297Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1007/s10493-016-0086-6
ISSN: 0168-8162
PubMed: 27631761
WoS: 000386075800003
Scopus: 2-s2.0-84986267458
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Gajić, Bojan AU - Stevanović, Jevrosima AU - Radulović, Željko AU - Kulišić, Zoran AU - Vejnović, Branislav AU - Glavinić, Uroš AU - Stanimirović, Zoran PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1422 AB - In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined. PB - Springer, Dordrecht T2 - Experimental and Applied Acarology T1 - Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods VL - 70 IS - 3 SP - 287 EP - 297 DO - 10.1007/s10493-016-0086-6 ER -
@article{ author = "Gajić, Bojan and Stevanović, Jevrosima and Radulović, Željko and Kulišić, Zoran and Vejnović, Branislav and Glavinić, Uroš and Stanimirović, Zoran", year = "2016", abstract = "In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.", publisher = "Springer, Dordrecht", journal = "Experimental and Applied Acarology", title = "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods", volume = "70", number = "3", pages = "287-297", doi = "10.1007/s10493-016-0086-6" }
Gajić, B., Stevanović, J., Radulović, Ž., Kulišić, Z., Vejnović, B., Glavinić, U.,& Stanimirović, Z.. (2016). Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology Springer, Dordrecht., 70(3), 287-297. https://doi.org/10.1007/s10493-016-0086-6
Gajić B, Stevanović J, Radulović Ž, Kulišić Z, Vejnović B, Glavinić U, Stanimirović Z. Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology. 2016;70(3):287-297. doi:10.1007/s10493-016-0086-6 .
Gajić, Bojan, Stevanović, Jevrosima, Radulović, Željko, Kulišić, Zoran, Vejnović, Branislav, Glavinić, Uroš, Stanimirović, Zoran, "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods" in Experimental and Applied Acarology, 70, no. 3 (2016):287-297, https://doi.org/10.1007/s10493-016-0086-6 . .