Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
2017
Authors
Stojić, MilicaFratrić, Natalija
Kovacić, Marijana
Ilić, Vesna
Gvozdić, Dragan
Savić, Olivera
Doković, Radojica
Valčić, Olivera
Article (Published version)
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Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determin...ed with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
Keywords:
agarose gel protein electrophoresis / brix refractometer / colostrum IgG / failure of passive transfer / new-born calf sera IgG / radial immunodiffusionSource:
Acta Veterinaria-Beograd, 2017, 67, 4, 508-524Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Biotechnology in the regulation of productive and reproductive status and health in dairy cows (RS-MESTD-Technological Development (TD or TR)-31050)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1515/acve-2017-0041
ISSN: 0567-8315
WoS: 000419653200005
Scopus: 2-s2.0-85041206212
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Stojić, Milica AU - Fratrić, Natalija AU - Kovacić, Marijana AU - Ilić, Vesna AU - Gvozdić, Dragan AU - Savić, Olivera AU - Doković, Radojica AU - Valčić, Olivera PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1519 AB - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer VL - 67 IS - 4 SP - 508 EP - 524 DO - 10.1515/acve-2017-0041 ER -
@article{ author = "Stojić, Milica and Fratrić, Natalija and Kovacić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valčić, Olivera", year = "2017", abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer", volume = "67", number = "4", pages = "508-524", doi = "10.1515/acve-2017-0041" }
Stojić, M., Fratrić, N., Kovacić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valčić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524. https://doi.org/10.1515/acve-2017-0041
Stojić M, Fratrić N, Kovacić M, Ilić V, Gvozdić D, Savić O, Doković R, Valčić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524. doi:10.1515/acve-2017-0041 .
Stojić, Milica, Fratrić, Natalija, Kovacić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valčić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524, https://doi.org/10.1515/acve-2017-0041 . .