Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay
2017
Аутори
Stević, NatašaMišić, Dušan
Bogunović, Danica
Matović, Kazimir
Valčić, Miroslav
Milovanović, Milovan
Radojičić, Sonja
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first ch...oice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.
Кључне речи:
Brucella canis / Bruce-ladder / dogs / reproductive organsИзвор:
Acta Veterinaria-Beograd, 2017, 67, 4, 551-561Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Развој и стандардизација молекуларних и имунских метода у дијагностици бактеријских зооноза (RS-MESTD-Technological Development (TD or TR)-31088)
DOI: 10.1515/acve-2017-0046
ISSN: 0567-8315
WoS: 000419653200008
Scopus: 2-s2.0-85041632142
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stević, Nataša AU - Mišić, Dušan AU - Bogunović, Danica AU - Matović, Kazimir AU - Valčić, Miroslav AU - Milovanović, Milovan AU - Radojičić, Sonja PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1544 AB - The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay VL - 67 IS - 4 SP - 551 EP - 561 DO - 10.1515/acve-2017-0046 ER -
@article{ author = "Stević, Nataša and Mišić, Dušan and Bogunović, Danica and Matović, Kazimir and Valčić, Miroslav and Milovanović, Milovan and Radojičić, Sonja", year = "2017", abstract = "The goal of this study was to compare the results of serological and conventional bacteriological methods with the results obtained using multiplex PCR Bruce-ladder assay. Based on the obtained results, the usability of the assay was assessed in regard to rapid diagnosis of canine brucellosis directly from the samples of reproductive organs of infected dogs. Out of 225 blood samples, 33 (14.67%) had a positive agglutination reaction. In this study, out of the 225 assayed reproductive organs of dogs, B. canis was isolated from 3 samples (1.33%), while the PCR Bruce-ladder assay detected two positive samples (0.88%). Two dogs from which B. canis was isolated, an antibody titer of 1/200 was established in blood serums, and third dog from which B. canis was isolated was negative using the tube agglutination test. From a total of 225 assayed organ samples, a positive PCR reaction was obtained from two samples. The obtained results show that the tube agglutination method remains the first choice for the detection of dogs infected with B. canis. In addition, whenever possible, it is necessary to try isolation. It is desirable to attempt the detection of B. canis in tissues using PCR, but the results may not be treated as definitive and reliable.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay", volume = "67", number = "4", pages = "551-561", doi = "10.1515/acve-2017-0046" }
Stević, N., Mišić, D., Bogunović, D., Matović, K., Valčić, M., Milovanović, M.,& Radojičić, S.. (2017). Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 551-561. https://doi.org/10.1515/acve-2017-0046
Stević N, Mišić D, Bogunović D, Matović K, Valčić M, Milovanović M, Radojičić S. Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay. in Acta Veterinaria-Beograd. 2017;67(4):551-561. doi:10.1515/acve-2017-0046 .
Stević, Nataša, Mišić, Dušan, Bogunović, Danica, Matović, Kazimir, Valčić, Miroslav, Milovanović, Milovan, Radojičić, Sonja, "Examining the possibility of detecting brucella canis from tissue samples using bruce-ladder multiplex PCR assay" in Acta Veterinaria-Beograd, 67, no. 4 (2017):551-561, https://doi.org/10.1515/acve-2017-0046 . .