N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro
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2018
Authors
Martacić, JasminaKovačević-Filipović, Milica
Borozan, Sunčica
Cvetković, Zorica
Popović, Tamara
Arsić, Aleksandra
Takić, Marija
Vucić, Vesna
Glibetić, Maria
Article (Published version)
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Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in compariso...n to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.
Keywords:
N-acetyl-L-cysteine / Exfoliated deciduous teeth stem cells / Lipid peroxidation / Fatty acid compositionSource:
Clinical Oral Investigations, 2018, 22, 8, 2897-2903Publisher:
- Springer Heidelberg, Heidelberg
Funding / projects:
- Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41030)
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1007/s00784-018-2377-2
ISSN: 1432-6981
PubMed: 29450735
WoS: 000452307800018
Scopus: 2-s2.0-85042068195
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Fakultet veterinarske medicineTY - JOUR AU - Martacić, Jasmina AU - Kovačević-Filipović, Milica AU - Borozan, Sunčica AU - Cvetković, Zorica AU - Popović, Tamara AU - Arsić, Aleksandra AU - Takić, Marija AU - Vucić, Vesna AU - Glibetić, Maria PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1573 AB - Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro. PB - Springer Heidelberg, Heidelberg T2 - Clinical Oral Investigations T1 - N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro VL - 22 IS - 8 SP - 2897 EP - 2903 DO - 10.1007/s00784-018-2377-2 ER -
@article{ author = "Martacić, Jasmina and Kovačević-Filipović, Milica and Borozan, Sunčica and Cvetković, Zorica and Popović, Tamara and Arsić, Aleksandra and Takić, Marija and Vucić, Vesna and Glibetić, Maria", year = "2018", abstract = "Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.", publisher = "Springer Heidelberg, Heidelberg", journal = "Clinical Oral Investigations", title = "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro", volume = "22", number = "8", pages = "2897-2903", doi = "10.1007/s00784-018-2377-2" }
Martacić, J., Kovačević-Filipović, M., Borozan, S., Cvetković, Z., Popović, T., Arsić, A., Takić, M., Vucić, V.,& Glibetić, M.. (2018). N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations Springer Heidelberg, Heidelberg., 22(8), 2897-2903. https://doi.org/10.1007/s00784-018-2377-2
Martacić J, Kovačević-Filipović M, Borozan S, Cvetković Z, Popović T, Arsić A, Takić M, Vucić V, Glibetić M. N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations. 2018;22(8):2897-2903. doi:10.1007/s00784-018-2377-2 .
Martacić, Jasmina, Kovačević-Filipović, Milica, Borozan, Sunčica, Cvetković, Zorica, Popović, Tamara, Arsić, Aleksandra, Takić, Marija, Vucić, Vesna, Glibetić, Maria, "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro" in Clinical Oral Investigations, 22, no. 8 (2018):2897-2903, https://doi.org/10.1007/s00784-018-2377-2 . .