The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis
2018
Authors
Polaček, VladimirVidanović, Dejan
Božić, B.
Becskei, Zsolt
Vučićević, Ivana
Prodanov-Radulović, Jasna
Aleksić-Kovačević, Sanja
Article (Published version)
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Show full item recordAbstract
The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. h...ominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.
Keywords:
Granuloma / Mycobacterium avium subsp. hominissuis / Myofibroblast / TGF-β1 / TGF-β1RISource:
Macedonian Veterinary Review, 2018, 41, 1, 47-53Publisher:
- Faculty of Veterinary Medicine - Skopje
Funding / projects:
- Research on pharmacological characteristics of antimicrobial agents, introduction of new technological solutions and alternative prophylactic methods with the purpose to improve control of infectious animal disease (RS-MESTD-Technological Development (TD or TR)-31071)
- Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination (RS-MESTD-Technological Development (TD or TR)-31084)
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
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Fakultet veterinarske medicineTY - JOUR AU - Polaček, Vladimir AU - Vidanović, Dejan AU - Božić, B. AU - Becskei, Zsolt AU - Vučićević, Ivana AU - Prodanov-Radulović, Jasna AU - Aleksić-Kovačević, Sanja PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1598 AB - The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH. PB - Faculty of Veterinary Medicine - Skopje T2 - Macedonian Veterinary Review T1 - The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis VL - 41 IS - 1 SP - 47 EP - 53 DO - 10.1515/macvetrev-2017-0030 ER -
@article{ author = "Polaček, Vladimir and Vidanović, Dejan and Božić, B. and Becskei, Zsolt and Vučićević, Ivana and Prodanov-Radulović, Jasna and Aleksić-Kovačević, Sanja", year = "2018", abstract = "The most important morphological characteristic of infections caused by M. avium subsp. hominissuis (MAH) is granuloma formation. The growth of mycobacteria is in accordance with anti-bacterial effector mechanisms of the host within granuloma. The most important cytokines for „orchestrating“the host defense are interferon γ (INF-γ), tumor necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1). Myofibroblasts that make up a peripheral layer of granuloma largely express receptors for TGF-β1. This cytokine is believed to affect the induction of myofibroblast proliferation. The aim of this paper is to point out the importance of myofibroblasts in the formation and sustainability of granuloma during natural infection of pigs with M. avium subsp. hominissuis. Examinations have been performed on the samples of Lnn. jejunales, Lnn. ileocolici and Lnn. colici of 100 pigs with a positive tuberculin skin test. The molecular method confirmed the presence of a genome M. avium subsp. hominissuis. The microscopic examination of lymph node samples stained by the routine hematoxyilin-eosin (HE) method, showed the presence of granulomatous lymphadenitis. The method of double immunohistochemical staining revealed that myofibroblasts which express TGF-β1 receptor type I (TGF-β1RI) and α smooth muscle actin (α SMA) have an important role in the morphogenesis of granulomatous lymphadenitis in pigs infected with MAH.", publisher = "Faculty of Veterinary Medicine - Skopje", journal = "Macedonian Veterinary Review", title = "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis", volume = "41", number = "1", pages = "47-53", doi = "10.1515/macvetrev-2017-0030" }
Polaček, V., Vidanović, D., Božić, B., Becskei, Z., Vučićević, I., Prodanov-Radulović, J.,& Aleksić-Kovačević, S.. (2018). The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review Faculty of Veterinary Medicine - Skopje., 41(1), 47-53. https://doi.org/10.1515/macvetrev-2017-0030
Polaček V, Vidanović D, Božić B, Becskei Z, Vučićević I, Prodanov-Radulović J, Aleksić-Kovačević S. The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis. in Macedonian Veterinary Review. 2018;41(1):47-53. doi:10.1515/macvetrev-2017-0030 .
Polaček, Vladimir, Vidanović, Dejan, Božić, B., Becskei, Zsolt, Vučićević, Ivana, Prodanov-Radulović, Jasna, Aleksić-Kovačević, Sanja, "The role of myofibroblasts in granulomatous lymphadenitis in pigs naturally infected with M. Avium subsp. hominissuis" in Macedonian Veterinary Review, 41, no. 1 (2018):47-53, https://doi.org/10.1515/macvetrev-2017-0030 . .