Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
Samo za registrovane korisnike
2018
Autori
Todorović, DaliborVelhner, Maja
Grego, Edita
Vidanović, Dejan
Milanov, Dubravka
Krnjaić, Dejan
Kehrenberg, Corinna
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr a...nd qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.
Ključne reči:
E / coli / pigs / cow milk / PFGE / resistance genes / integrons / conjugative plasmidsIzvor:
Microbial Drug Resistance, 2018, 24, 1, 95-103Izdavač:
- Mary Ann Liebert, Inc, New Rochelle
Finansiranje / projekti:
- Istraživanje farmakoloških karakteristika antimikrobnih agenasa, uvođenje novih tehnoloških rešenja i alternativnih metoda profilakse s ciljem da se poboljša kontrola infektivnih oboljenja domaćih životinja (RS-MESTD-Technological Development (TD or TR)-31071)
DOI: 10.1089/mdr.2017.0016
ISSN: 1076-6294
PubMed: 28520501
WoS: 000419819100015
Scopus: 2-s2.0-85040590273
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Todorović, Dalibor AU - Velhner, Maja AU - Grego, Edita AU - Vidanović, Dejan AU - Milanov, Dubravka AU - Krnjaić, Dejan AU - Kehrenberg, Corinna PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1648 AB - The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry. PB - Mary Ann Liebert, Inc, New Rochelle T2 - Microbial Drug Resistance T1 - Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia VL - 24 IS - 1 SP - 95 EP - 103 DO - 10.1089/mdr.2017.0016 ER -
@article{ author = "Todorović, Dalibor and Velhner, Maja and Grego, Edita and Vidanović, Dejan and Milanov, Dubravka and Krnjaić, Dejan and Kehrenberg, Corinna", year = "2018", abstract = "The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.", publisher = "Mary Ann Liebert, Inc, New Rochelle", journal = "Microbial Drug Resistance", title = "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia", volume = "24", number = "1", pages = "95-103", doi = "10.1089/mdr.2017.0016" }
Todorović, D., Velhner, M., Grego, E., Vidanović, D., Milanov, D., Krnjaić, D.,& Kehrenberg, C.. (2018). Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance Mary Ann Liebert, Inc, New Rochelle., 24(1), 95-103. https://doi.org/10.1089/mdr.2017.0016
Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance. 2018;24(1):95-103. doi:10.1089/mdr.2017.0016 .
Todorović, Dalibor, Velhner, Maja, Grego, Edita, Vidanović, Dejan, Milanov, Dubravka, Krnjaić, Dejan, Kehrenberg, Corinna, "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia" in Microbial Drug Resistance, 24, no. 1 (2018):95-103, https://doi.org/10.1089/mdr.2017.0016 . .