Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
2019
Authors
Ašanin, JelenaMišić, Dušan
Aksentijević, Ksenija
Tambur, Zoran
Rakonjac, Bojan
Kovačević, Ivana
Spergser, Joachim
Loncarić, Igor
Article (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A),... erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.
Keywords:
MRSA / humans / animals / antibiotic resistance / SCCmec typing / dru typing / spa typing / MLVA / MLSTSource:
Antibiotics-Basel, 2019, 8, 1, 26-Publisher:
- MDPI, Basel
Funding / projects:
- Implementation and evaluation of a new molecular method for a quick detection of a mecA gene directly in swabs originated from humans, animals and their environment (RS-MESTD-Technological Development (TD or TR)-31079)
DOI: 10.3390/antibiotics8010026
ISSN: 2079-6382
PubMed: 30884836
WoS: 000464147600002
Scopus: 2-s2.0-85064243952
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Ašanin, Jelena AU - Mišić, Dušan AU - Aksentijević, Ksenija AU - Tambur, Zoran AU - Rakonjac, Bojan AU - Kovačević, Ivana AU - Spergser, Joachim AU - Loncarić, Igor PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1758 AB - The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission. PB - MDPI, Basel T2 - Antibiotics-Basel T1 - Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia VL - 8 IS - 1 SP - 26 DO - 10.3390/antibiotics8010026 ER -
@article{ author = "Ašanin, Jelena and Mišić, Dušan and Aksentijević, Ksenija and Tambur, Zoran and Rakonjac, Bojan and Kovačević, Ivana and Spergser, Joachim and Loncarić, Igor", year = "2019", abstract = "The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.", publisher = "MDPI, Basel", journal = "Antibiotics-Basel", title = "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia", volume = "8", number = "1", pages = "26", doi = "10.3390/antibiotics8010026" }
Ašanin, J., Mišić, D., Aksentijević, K., Tambur, Z., Rakonjac, B., Kovačević, I., Spergser, J.,& Loncarić, I.. (2019). Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel MDPI, Basel., 8(1), 26. https://doi.org/10.3390/antibiotics8010026
Ašanin J, Mišić D, Aksentijević K, Tambur Z, Rakonjac B, Kovačević I, Spergser J, Loncarić I. Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel. 2019;8(1):26. doi:10.3390/antibiotics8010026 .
Ašanin, Jelena, Mišić, Dušan, Aksentijević, Ksenija, Tambur, Zoran, Rakonjac, Bojan, Kovačević, Ivana, Spergser, Joachim, Loncarić, Igor, "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia" in Antibiotics-Basel, 8, no. 1 (2019):26, https://doi.org/10.3390/antibiotics8010026 . .