Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus
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2020
Authors
Cvetković, MirjanaDamjanović, Ana
Stanojković, Tatjana P.
Đorđević, Iris
Tešević, Vele
Milosavljević, Slobodan M.
Gođevac, Dejan
Article (Published version)
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Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using thy-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIRbased metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.
Keywords:
Amphoricarpos autariatus / NMR / FTIR / Metabolomics / Cytotoxic activity / Dry-column flash chromatographySource:
Talanta, 2020, 206, 120248-Publisher:
- Elsevier, Amsterdam
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-MESTD-Basic Research (BR or ON)-172053)
DOI: 10.1016/j.talanta.2019.120248
ISSN: 0039-9140
PubMed: 31514863
WoS: 000488416200070
Scopus: 2-s2.0-85070705746
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Fakultet veterinarske medicineTY - JOUR AU - Cvetković, Mirjana AU - Damjanović, Ana AU - Stanojković, Tatjana P. AU - Đorđević, Iris AU - Tešević, Vele AU - Milosavljević, Slobodan M. AU - Gođevac, Dejan PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1802 AB - Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using thy-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIRbased metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed. PB - Elsevier, Amsterdam T2 - Talanta T1 - Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus VL - 206 SP - 120248 DO - 10.1016/j.talanta.2019.120248 ER -
@article{ author = "Cvetković, Mirjana and Damjanović, Ana and Stanojković, Tatjana P. and Đorđević, Iris and Tešević, Vele and Milosavljević, Slobodan M. and Gođevac, Dejan", year = "2020", abstract = "Metabolomics generate a profile of small molecules from plant extracts, which could be directly responsible for bioactivity effects. Using thy-column flash chromatography enabled a rapid and inexpensive method for the very efficient separation of plant extract with a high resolution. This separation method coupled to NMR and FTIRbased metabolomics is applied to identify bioactive natural products. OPLS multivariate analysis method, was used for correlation the chemical composition of the plant extracts, Amphoricarpos autariatus, with the results of cytotoxic activity against Human cervical adenocarcinoma cell line (HeLa) and epithelial lung cancer cell line (A549). In this way, the highest contribution to the cytotoxic activity was recorded for the guaianolide sesquiterpene lactones named amphoricarpolides. The compounds indicated as bioactive after metabolomics analysis were tested, and their cytotoxic activity were confirmed.", publisher = "Elsevier, Amsterdam", journal = "Talanta", title = "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus", volume = "206", pages = "120248", doi = "10.1016/j.talanta.2019.120248" }
Cvetković, M., Damjanović, A., Stanojković, T. P., Đorđević, I., Tešević, V., Milosavljević, S. M.,& Gođevac, D.. (2020). Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta Elsevier, Amsterdam., 206, 120248. https://doi.org/10.1016/j.talanta.2019.120248
Cvetković M, Damjanović A, Stanojković TP, Đorđević I, Tešević V, Milosavljević SM, Gođevac D. Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus. in Talanta. 2020;206:120248. doi:10.1016/j.talanta.2019.120248 .
Cvetković, Mirjana, Damjanović, Ana, Stanojković, Tatjana P., Đorđević, Iris, Tešević, Vele, Milosavljević, Slobodan M., Gođevac, Dejan, "Integration of dry-column flash chromatography with NMR and FTIR metabolomics to reveal cytotoxic metabolites from Amphoricarpos autariatus" in Talanta, 206 (2020):120248, https://doi.org/10.1016/j.talanta.2019.120248 . .