Influence of parenteral application of beta-carotene on fertility in cows

Abstract

U zapatu visokomlečnih krava holštajn rase odabrano je 30 krava za izvođenje ogleda. Životinje su odabrane u periodu visokog graviditeta i podeljene su u dve grupe. Kravama ogledne grupe (n=15) je dve nedelje pre očekivanog datuma partusa aplikovan i.m preparat betakarotina, Carofertin, u količini od 20 ml (200 mg beta-karotina). Dve nedelje posle partusa, kravama ogledne grupe je aplikovana druga injekcija preparata beta-karotina. U isto vreme, kravama kontrolne grupe (n=15) i.m je ubrizgan fiziološki rastvor u količini od 20 ml. Uzorci krvi za ispitivanje koncentracije beta-karotina i vitamina A su uzimani u periodu visokog graviditeta i u ranom puerperijumu drugoga dana posle aplikacije preparata beta-karotina, kao i na sam dan partusa. Dvokratno parenteralno davanje beta-karotina u peripartalnom periodu ne utiče značajno na učestalost pojavljivanja zadržane posteljice kod krava ogledne, u poređenju sa kravama kontrolne grupe (x=33,3% i x=20,0 %) i dužinu trajanja servis perioda (×=97,20±31,64 i ×= 98,8±35,8 dana). Na osnovu rezultata dobijenih u radu može se zaključiti da parenteralno davanje preparata beta-karotina (Carofertin), koji je namenjen za profilaksu reproduktivnih poremećaja goveda, u dozi od 200 mg po životinji nema uticaja na nivo karotinemije u uslovima kada je njegova koncentracija u krvnom serumu u fiziološkim granicama. Isto tako, nije ustanovljena značajna razlika u vrednostima reproduktivnih pokazatelja između krava ogledne i kontrolne grupe. Po svemu sudeći, njegova upotreba je preporučljiva samo tokom perioda ishrane životinja hranom koja ne sadrži dovoljne količine ovog provitamina.

Thirty Holstein breed cows from the breeding stock of high yielding dairy cattle were selected for testing. The cows were selected during their high gravidity and fell into two groups. The i.m beta-carotene, Carofertin, of 20 ml (200 mg of beta-carotene) was ap plied to the experimental group (n=15) two weeks before the expected parturition date. Another injection of the beta-carotene preparation was applied to the experimental group two weeks after parturition. Simultaneously, a physiological solution of 20 ml was injected i.m to the experimental group (n=15). The blood samples for testing of the beta-carotene and vitamin A concentration were taken during high gravidity and in the early puerperium two days after the beta-carotene application and on the day of parturition. Two-of parenteral application of beta-carotene before parturition does not have an impact significantly on the frequency of the occurrence of retained placenta in experimental cows comparing to the cows of the control group (x=33.3 % and x=20.0 %) and length of the service period (x =97.20±31.64 and x=98.8±35.8 days). Based on the results obtained during this research, it can be concluded that the parenteral application of the beta-carotene (Carofertin) as 200 mg per cow, which is meant for protection of the reproductive disorders of cows, does not affect the level of carotinemia when its concentration in the blood serum is within the physiological limits. Therefore, no significant discrepancy in values of the reproduction figures between the cows of the experimental and control group was found. All in all, its usage is advisable only during scarce feeding.