Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia
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2020
Authors
Milićević, VesnaKureljušić, Branislav
Maksimović-Zorić, Jelena
Savić, Božidar
Spalević, Ljiljana
Žutić, Jadranka
Article (Published version)
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Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have... to be considered when PKV infection is diagnosed.
Keywords:
Domestic pigs / Porcine Kobuvirus / Serbia / Wild boarsSource:
Research in Veterinary Science, 2020, 132, 404-406Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200030 (Scientific Veterinary Institute of Serbia, Belgrade) (RS-MESTD-inst-2020-200030)
DOI: 10.1016/j.rvsc.2020.07.028
ISSN: 0034-5288
WoS: 000567455400006
Scopus: 2-s2.0-85088915075
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Fakultet veterinarske medicineTY - JOUR AU - Milićević, Vesna AU - Kureljušić, Branislav AU - Maksimović-Zorić, Jelena AU - Savić, Božidar AU - Spalević, Ljiljana AU - Žutić, Jadranka PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1985 AB - Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed. PB - Elsevier T2 - Research in Veterinary Science T1 - Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia VL - 132 SP - 404 EP - 406 DO - 10.1016/j.rvsc.2020.07.028 ER -
@article{ author = "Milićević, Vesna and Kureljušić, Branislav and Maksimović-Zorić, Jelena and Savić, Božidar and Spalević, Ljiljana and Žutić, Jadranka", year = "2020", abstract = "Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.", publisher = "Elsevier", journal = "Research in Veterinary Science", title = "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia", volume = "132", pages = "404-406", doi = "10.1016/j.rvsc.2020.07.028" }
Milićević, V., Kureljušić, B., Maksimović-Zorić, J., Savić, B., Spalević, L.,& Žutić, J.. (2020). Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science Elsevier., 132, 404-406. https://doi.org/10.1016/j.rvsc.2020.07.028
Milićević V, Kureljušić B, Maksimović-Zorić J, Savić B, Spalević L, Žutić J. Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia. in Research in Veterinary Science. 2020;132:404-406. doi:10.1016/j.rvsc.2020.07.028 .
Milićević, Vesna, Kureljušić, Branislav, Maksimović-Zorić, Jelena, Savić, Božidar, Spalević, Ljiljana, Žutić, Jadranka, "Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia" in Research in Veterinary Science, 132 (2020):404-406, https://doi.org/10.1016/j.rvsc.2020.07.028 . .