Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs
Само за регистроване кориснике
2021
Аутори
Spariosu, KristinaJanjić, Filip
Radaković, Milena
Francuski Andrić, Jelena
Beletić, Anđelo
Kovačević-Filipović, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Inflammation is a hallmark of the acute Babesia canis infection. Promatrix metalloproteinase (proMMP)-2 and -9 are involved in inflammation, but their levels have not been analyzed in canine babesiosis. We hypothesized that in dogs infected with B. canis, serum proMMP-2 and -9 levels change between presentation and recovery. Degree of the change differs if dogs develop systemic inflammatory response syndrome (SIRS). This study included 24 dogs with an acute B. canis infection, at presentation and after two weeks. We used routine hematology and biochemistry methods, spectrophotometry for the acute-phase proteins, microscopy for parasitemia and zymography for (pro)MMPs. In vitro endothelial cells and leukocyte short-term cultures, and platelet lysates were used to detect specific MMP activity. Statistical analyses included Wilcoxon test for paired samples, Mann-Whitney U test and Spearman’s rank correlation. Our results showed that endothelial cells, leukocytes and platelets are the sour...ce of proMMP-2 and proMMP-9. Furthermore, both proMMPs were lower at presentation than after recovery (p < 0.001). At presentation, proMMP-9 levels correlated with parasitemia (rho = -0.616, p = 0.009), total leukocyte (rho = 0.704, p < 0.001) and neutrophil counts (rho = 0.741, p < 0.001). Extent of alterations in proMMP-2 levels between presentation and recovery was lower (p = 0.038) in dogs with SIRS than in non-SIRS dogs, while levels of proMMP-9 were comparable between these groups. Our conclusion is that during the acute B. canis infection, low serum levels of proMMP-2 and proMMP-9 at presentation reflect thrombocytopenia and leukopenia. Decreased proMMP-2 level could be associated with SIRS.
Кључне речи:
Endothelial cells / Inflammation / Leukopenia / Matrix metalloproteinases / ThrombocytopeniaИзвор:
Veterinary Parasitology, 2021, 300, 109612-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
DOI: 10.1016/j.vetpar.2021.109612
ISSN: 0304-4017
WoS: 000716916100011
Scopus: 2-s2.0-85118869475
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Spariosu, Kristina AU - Janjić, Filip AU - Radaković, Milena AU - Francuski Andrić, Jelena AU - Beletić, Anđelo AU - Kovačević-Filipović, Milica PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2240 AB - Inflammation is a hallmark of the acute Babesia canis infection. Promatrix metalloproteinase (proMMP)-2 and -9 are involved in inflammation, but their levels have not been analyzed in canine babesiosis. We hypothesized that in dogs infected with B. canis, serum proMMP-2 and -9 levels change between presentation and recovery. Degree of the change differs if dogs develop systemic inflammatory response syndrome (SIRS). This study included 24 dogs with an acute B. canis infection, at presentation and after two weeks. We used routine hematology and biochemistry methods, spectrophotometry for the acute-phase proteins, microscopy for parasitemia and zymography for (pro)MMPs. In vitro endothelial cells and leukocyte short-term cultures, and platelet lysates were used to detect specific MMP activity. Statistical analyses included Wilcoxon test for paired samples, Mann-Whitney U test and Spearman’s rank correlation. Our results showed that endothelial cells, leukocytes and platelets are the source of proMMP-2 and proMMP-9. Furthermore, both proMMPs were lower at presentation than after recovery (p < 0.001). At presentation, proMMP-9 levels correlated with parasitemia (rho = -0.616, p = 0.009), total leukocyte (rho = 0.704, p < 0.001) and neutrophil counts (rho = 0.741, p < 0.001). Extent of alterations in proMMP-2 levels between presentation and recovery was lower (p = 0.038) in dogs with SIRS than in non-SIRS dogs, while levels of proMMP-9 were comparable between these groups. Our conclusion is that during the acute B. canis infection, low serum levels of proMMP-2 and proMMP-9 at presentation reflect thrombocytopenia and leukopenia. Decreased proMMP-2 level could be associated with SIRS. PB - Elsevier T2 - Veterinary Parasitology T1 - Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs VL - 300 SP - 109612 DO - 10.1016/j.vetpar.2021.109612 ER -
@article{ author = "Spariosu, Kristina and Janjić, Filip and Radaković, Milena and Francuski Andrić, Jelena and Beletić, Anđelo and Kovačević-Filipović, Milica", year = "2021", abstract = "Inflammation is a hallmark of the acute Babesia canis infection. Promatrix metalloproteinase (proMMP)-2 and -9 are involved in inflammation, but their levels have not been analyzed in canine babesiosis. We hypothesized that in dogs infected with B. canis, serum proMMP-2 and -9 levels change between presentation and recovery. Degree of the change differs if dogs develop systemic inflammatory response syndrome (SIRS). This study included 24 dogs with an acute B. canis infection, at presentation and after two weeks. We used routine hematology and biochemistry methods, spectrophotometry for the acute-phase proteins, microscopy for parasitemia and zymography for (pro)MMPs. In vitro endothelial cells and leukocyte short-term cultures, and platelet lysates were used to detect specific MMP activity. Statistical analyses included Wilcoxon test for paired samples, Mann-Whitney U test and Spearman’s rank correlation. Our results showed that endothelial cells, leukocytes and platelets are the source of proMMP-2 and proMMP-9. Furthermore, both proMMPs were lower at presentation than after recovery (p < 0.001). At presentation, proMMP-9 levels correlated with parasitemia (rho = -0.616, p = 0.009), total leukocyte (rho = 0.704, p < 0.001) and neutrophil counts (rho = 0.741, p < 0.001). Extent of alterations in proMMP-2 levels between presentation and recovery was lower (p = 0.038) in dogs with SIRS than in non-SIRS dogs, while levels of proMMP-9 were comparable between these groups. Our conclusion is that during the acute B. canis infection, low serum levels of proMMP-2 and proMMP-9 at presentation reflect thrombocytopenia and leukopenia. Decreased proMMP-2 level could be associated with SIRS.", publisher = "Elsevier", journal = "Veterinary Parasitology", title = "Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs", volume = "300", pages = "109612", doi = "10.1016/j.vetpar.2021.109612" }
Spariosu, K., Janjić, F., Radaković, M., Francuski Andrić, J., Beletić, A.,& Kovačević-Filipović, M.. (2021). Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs. in Veterinary Parasitology Elsevier., 300, 109612. https://doi.org/10.1016/j.vetpar.2021.109612
Spariosu K, Janjić F, Radaković M, Francuski Andrić J, Beletić A, Kovačević-Filipović M. Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs. in Veterinary Parasitology. 2021;300:109612. doi:10.1016/j.vetpar.2021.109612 .
Spariosu, Kristina, Janjić, Filip, Radaković, Milena, Francuski Andrić, Jelena, Beletić, Anđelo, Kovačević-Filipović, Milica, "Low serum levels of promatrix metalloproteinase-2 and -9 occur during acute Babesia canis infection in dogs" in Veterinary Parasitology, 300 (2021):109612, https://doi.org/10.1016/j.vetpar.2021.109612 . .