Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia
Prisustvo gena za enerotoksine i emetični toksin i genetička raznolikost bakterije bacillus cereus izolovane iz hrane, okoline i kliničkih uzoraka u Srbiji
2020
Authors
Savić, DejanaRistanović, Elizabeta
Miljković Selimović, Biljana
Radaković, Sonja
Jošić, Dragana
Lepšanović, Zorica
Article (Published version)
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Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three gr...oups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.
Bacillus cereus se najčešće unosi hranom i usled prisustva toksina može izazvati povraćanje i dijareu. Sistemska infekcija je moguća. Cilj je bio da se utvrdi prisustvo gena za enterotoksine (hblA, entFM) i emetični toksin (cer) i istražiti genetsku heterogenost izolata B. cereus iz uzoraka hrane, životne sredine i stolice pacijenata. Identifi kacija B. cereus je urađena upotrebom selektivne hranljive podloge, klasičnih biohemijskih testova i lančane reakcije polimeraze (PCR). Detekcija gena je rađena PCR-om. Tipizacija je urađena pomoću nasumičnog umnožavanja polimorfne DNA (RAPD). EntFM gen je bio prisutan u svakom uzorku stolice i hrane, a u 28/30 izolata iz okoline. HblA gen je bio prisutan u 29/30 izolata stolice, 23/30 izolata hrane i u 24/30 izolata iz okoline. Cer gen je bio prisutan u svim izolatima stolice, 28/30 izolata hrane i 25/30 izolata iz okoline. RAPD rezultati pokazuju visoku heterogenost među izolatima iz svake grupe. Zbirni dendrogram dobijen od odabranih izolata ...iz sve tri ispitane grupe, pokazuje formiranje dva klastera sa međusobnom razlikom od 53%. Detekcija gena koji kodiraju prisustvo toksina u svim izolatima B. cereus, ukazuje da su ove bakterije potencijalno patogene i predstavljaju ozbiljnu pretnju po ljudsko zdravlje. Prisustvo izolata iz sve tri grupe u istom klasteru ukazuje na postojanje sličnih sojeva iz različitih izvora, što se dovodi u direktnu vezu sa kretanjem sojeva u prirodi kroz lanac ishrane.
Keywords:
Bacillus cereus / Emetic toxin gene / Enterotoxin genes / PCR / RAPD typingSource:
Acta Veterinaria-Beograd, 2020, 70, 2, 182-193Funding / projects:
- Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (RS-MESTD-Technological Development (TD or TR)-37006)
DOI: 10.2478/acve-2020-0013
ISSN: 0567-8315
WoS: 000540849900003
Scopus: 2-s2.0-85087829713
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Fakultet veterinarske medicineTY - JOUR AU - Savić, Dejana AU - Ristanović, Elizabeta AU - Miljković Selimović, Biljana AU - Radaković, Sonja AU - Jošić, Dragana AU - Lepšanović, Zorica PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2403 AB - Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain. AB - Bacillus cereus se najčešće unosi hranom i usled prisustva toksina može izazvati povraćanje i dijareu. Sistemska infekcija je moguća. Cilj je bio da se utvrdi prisustvo gena za enterotoksine (hblA, entFM) i emetični toksin (cer) i istražiti genetsku heterogenost izolata B. cereus iz uzoraka hrane, životne sredine i stolice pacijenata. Identifi kacija B. cereus je urađena upotrebom selektivne hranljive podloge, klasičnih biohemijskih testova i lančane reakcije polimeraze (PCR). Detekcija gena je rađena PCR-om. Tipizacija je urađena pomoću nasumičnog umnožavanja polimorfne DNA (RAPD). EntFM gen je bio prisutan u svakom uzorku stolice i hrane, a u 28/30 izolata iz okoline. HblA gen je bio prisutan u 29/30 izolata stolice, 23/30 izolata hrane i u 24/30 izolata iz okoline. Cer gen je bio prisutan u svim izolatima stolice, 28/30 izolata hrane i 25/30 izolata iz okoline. RAPD rezultati pokazuju visoku heterogenost među izolatima iz svake grupe. Zbirni dendrogram dobijen od odabranih izolata iz sve tri ispitane grupe, pokazuje formiranje dva klastera sa međusobnom razlikom od 53%. Detekcija gena koji kodiraju prisustvo toksina u svim izolatima B. cereus, ukazuje da su ove bakterije potencijalno patogene i predstavljaju ozbiljnu pretnju po ljudsko zdravlje. Prisustvo izolata iz sve tri grupe u istom klasteru ukazuje na postojanje sličnih sojeva iz različitih izvora, što se dovodi u direktnu vezu sa kretanjem sojeva u prirodi kroz lanac ishrane. T2 - Acta Veterinaria-Beograd T1 - Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia T1 - Prisustvo gena za enerotoksine i emetični toksin i genetička raznolikost bakterije bacillus cereus izolovane iz hrane, okoline i kliničkih uzoraka u Srbiji VL - 70 IS - 2 SP - 182 EP - 193 DO - 10.2478/acve-2020-0013 ER -
@article{ author = "Savić, Dejana and Ristanović, Elizabeta and Miljković Selimović, Biljana and Radaković, Sonja and Jošić, Dragana and Lepšanović, Zorica", year = "2020", abstract = "Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain., Bacillus cereus se najčešće unosi hranom i usled prisustva toksina može izazvati povraćanje i dijareu. Sistemska infekcija je moguća. Cilj je bio da se utvrdi prisustvo gena za enterotoksine (hblA, entFM) i emetični toksin (cer) i istražiti genetsku heterogenost izolata B. cereus iz uzoraka hrane, životne sredine i stolice pacijenata. Identifi kacija B. cereus je urađena upotrebom selektivne hranljive podloge, klasičnih biohemijskih testova i lančane reakcije polimeraze (PCR). Detekcija gena je rađena PCR-om. Tipizacija je urađena pomoću nasumičnog umnožavanja polimorfne DNA (RAPD). EntFM gen je bio prisutan u svakom uzorku stolice i hrane, a u 28/30 izolata iz okoline. HblA gen je bio prisutan u 29/30 izolata stolice, 23/30 izolata hrane i u 24/30 izolata iz okoline. Cer gen je bio prisutan u svim izolatima stolice, 28/30 izolata hrane i 25/30 izolata iz okoline. RAPD rezultati pokazuju visoku heterogenost među izolatima iz svake grupe. Zbirni dendrogram dobijen od odabranih izolata iz sve tri ispitane grupe, pokazuje formiranje dva klastera sa međusobnom razlikom od 53%. Detekcija gena koji kodiraju prisustvo toksina u svim izolatima B. cereus, ukazuje da su ove bakterije potencijalno patogene i predstavljaju ozbiljnu pretnju po ljudsko zdravlje. Prisustvo izolata iz sve tri grupe u istom klasteru ukazuje na postojanje sličnih sojeva iz različitih izvora, što se dovodi u direktnu vezu sa kretanjem sojeva u prirodi kroz lanac ishrane.", journal = "Acta Veterinaria-Beograd", title = "Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia, Prisustvo gena za enerotoksine i emetični toksin i genetička raznolikost bakterije bacillus cereus izolovane iz hrane, okoline i kliničkih uzoraka u Srbiji", volume = "70", number = "2", pages = "182-193", doi = "10.2478/acve-2020-0013" }
Savić, D., Ristanović, E., Miljković Selimović, B., Radaković, S., Jošić, D.,& Lepšanović, Z.. (2020). Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia. in Acta Veterinaria-Beograd, 70(2), 182-193. https://doi.org/10.2478/acve-2020-0013
Savić D, Ristanović E, Miljković Selimović B, Radaković S, Jošić D, Lepšanović Z. Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia. in Acta Veterinaria-Beograd. 2020;70(2):182-193. doi:10.2478/acve-2020-0013 .
Savić, Dejana, Ristanović, Elizabeta, Miljković Selimović, Biljana, Radaković, Sonja, Jošić, Dragana, Lepšanović, Zorica, "Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia" in Acta Veterinaria-Beograd, 70, no. 2 (2020):182-193, https://doi.org/10.2478/acve-2020-0013 . .