Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia
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2022
Authors
Arsić, MilošVićić, Ivan
Galić, Nataša
Dmitrić, Marko
Kureljušić, Jasna
Dimitrijević, Mirjana
Petrović, Miloš
Šarić, Ljubiša
Karabasil, Nedjeljko
Article (Published version)
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A survey was undertaken to determine the overall prevalence of Yersinia enterocolitica in pigs of slaughter age and to characterize the isolates in relation to bio-serotype, the presence of virulence genes, genetic diversity, and antimicrobial resistance. Moreover, possible risk factors associated with Y. enterocolitica infection during the pre-harvested and harvested phase of pig production were studied. The overall Y. enterocolitica prevalence in the pigs was 10.4% (95% confidence interval, CI = 8.5–12.3%). The most common Y. enterocolitica bio-serotype was 4/O:3, accounting for 81.6% of investigated isolates. The pathogenicity of 63 Y. enterocolitica 4/O:3 isolates, originating from all infected farms, was confirmed by the presence of both the ail and ystA virulence-associated genes and the absence of ystB gene (100%). Characterization with PFGE of 63 confirmed Y. enterocolitica 4/O:3 isolates identified five different genotypes with shared identical genetic profiles (100% similarit...y) within each genotype. Isolates originating from farrow-to-finish farms were only resistant to ampicillin, while resistance to nalidixic acid, tetracycline, and chloramphenicol at fattening farms was also observed. Risk factors related to Y. enterocolitica pig infection include fattening farms (odds ratio, OR = 2.3, 95% CI = 1.4–3.8, P < 0.001), a 3–6 h lairage period (OR = 1.6, 95% CI = 1.0–2.6, P = 0.035) and winter season (OR = 3.8, 95% CI = 2.0–7.4, P < 0.001). In addition to the overall characterization of Y. enterocolitica isolates, identification of the main risks associated with infection allows better application of preventive measures to reduce the occurrence and distribution of Y. enterocolitica infection.
Keywords:
Antimicrobial resistance / Bio-serotyping / PFGE / Virulence genesSource:
Research in Veterinary Science, 2022, 152, 167-174Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
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Fakultet veterinarske medicineTY - JOUR AU - Arsić, Miloš AU - Vićić, Ivan AU - Galić, Nataša AU - Dmitrić, Marko AU - Kureljušić, Jasna AU - Dimitrijević, Mirjana AU - Petrović, Miloš AU - Šarić, Ljubiša AU - Karabasil, Nedjeljko PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2496 AB - A survey was undertaken to determine the overall prevalence of Yersinia enterocolitica in pigs of slaughter age and to characterize the isolates in relation to bio-serotype, the presence of virulence genes, genetic diversity, and antimicrobial resistance. Moreover, possible risk factors associated with Y. enterocolitica infection during the pre-harvested and harvested phase of pig production were studied. The overall Y. enterocolitica prevalence in the pigs was 10.4% (95% confidence interval, CI = 8.5–12.3%). The most common Y. enterocolitica bio-serotype was 4/O:3, accounting for 81.6% of investigated isolates. The pathogenicity of 63 Y. enterocolitica 4/O:3 isolates, originating from all infected farms, was confirmed by the presence of both the ail and ystA virulence-associated genes and the absence of ystB gene (100%). Characterization with PFGE of 63 confirmed Y. enterocolitica 4/O:3 isolates identified five different genotypes with shared identical genetic profiles (100% similarity) within each genotype. Isolates originating from farrow-to-finish farms were only resistant to ampicillin, while resistance to nalidixic acid, tetracycline, and chloramphenicol at fattening farms was also observed. Risk factors related to Y. enterocolitica pig infection include fattening farms (odds ratio, OR = 2.3, 95% CI = 1.4–3.8, P < 0.001), a 3–6 h lairage period (OR = 1.6, 95% CI = 1.0–2.6, P = 0.035) and winter season (OR = 3.8, 95% CI = 2.0–7.4, P < 0.001). In addition to the overall characterization of Y. enterocolitica isolates, identification of the main risks associated with infection allows better application of preventive measures to reduce the occurrence and distribution of Y. enterocolitica infection. PB - Elsevier T2 - Research in Veterinary Science T1 - Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia VL - 152 SP - 167 EP - 174 DO - 10.1016/j.rvsc.2022.08.007 ER -
@article{ author = "Arsić, Miloš and Vićić, Ivan and Galić, Nataša and Dmitrić, Marko and Kureljušić, Jasna and Dimitrijević, Mirjana and Petrović, Miloš and Šarić, Ljubiša and Karabasil, Nedjeljko", year = "2022", abstract = "A survey was undertaken to determine the overall prevalence of Yersinia enterocolitica in pigs of slaughter age and to characterize the isolates in relation to bio-serotype, the presence of virulence genes, genetic diversity, and antimicrobial resistance. Moreover, possible risk factors associated with Y. enterocolitica infection during the pre-harvested and harvested phase of pig production were studied. The overall Y. enterocolitica prevalence in the pigs was 10.4% (95% confidence interval, CI = 8.5–12.3%). The most common Y. enterocolitica bio-serotype was 4/O:3, accounting for 81.6% of investigated isolates. The pathogenicity of 63 Y. enterocolitica 4/O:3 isolates, originating from all infected farms, was confirmed by the presence of both the ail and ystA virulence-associated genes and the absence of ystB gene (100%). Characterization with PFGE of 63 confirmed Y. enterocolitica 4/O:3 isolates identified five different genotypes with shared identical genetic profiles (100% similarity) within each genotype. Isolates originating from farrow-to-finish farms were only resistant to ampicillin, while resistance to nalidixic acid, tetracycline, and chloramphenicol at fattening farms was also observed. Risk factors related to Y. enterocolitica pig infection include fattening farms (odds ratio, OR = 2.3, 95% CI = 1.4–3.8, P < 0.001), a 3–6 h lairage period (OR = 1.6, 95% CI = 1.0–2.6, P = 0.035) and winter season (OR = 3.8, 95% CI = 2.0–7.4, P < 0.001). In addition to the overall characterization of Y. enterocolitica isolates, identification of the main risks associated with infection allows better application of preventive measures to reduce the occurrence and distribution of Y. enterocolitica infection.", publisher = "Elsevier", journal = "Research in Veterinary Science", title = "Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia", volume = "152", pages = "167-174", doi = "10.1016/j.rvsc.2022.08.007" }
Arsić, M., Vićić, I., Galić, N., Dmitrić, M., Kureljušić, J., Dimitrijević, M., Petrović, M., Šarić, L.,& Karabasil, N.. (2022). Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia. in Research in Veterinary Science Elsevier., 152, 167-174. https://doi.org/10.1016/j.rvsc.2022.08.007
Arsić M, Vićić I, Galić N, Dmitrić M, Kureljušić J, Dimitrijević M, Petrović M, Šarić L, Karabasil N. Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia. in Research in Veterinary Science. 2022;152:167-174. doi:10.1016/j.rvsc.2022.08.007 .
Arsić, Miloš, Vićić, Ivan, Galić, Nataša, Dmitrić, Marko, Kureljušić, Jasna, Dimitrijević, Mirjana, Petrović, Miloš, Šarić, Ljubiša, Karabasil, Nedjeljko, "Risk factors and the overall characterization of Yersinia enterocolitica as an initial model of pathogen surveillance in the pig production system in Serbia" in Research in Veterinary Science, 152 (2022):167-174, https://doi.org/10.1016/j.rvsc.2022.08.007 . .