Ovarian surface epithelium in newborn rats: germ cells warehouse
Authors
Danilović Luković, JelenaKorać, Aleksandra
Milošević, Ivan
Lužajić, Tijana
Kovačević Filipović, Milica
Radovanović, Anita
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Ovarian surface epithelium (OSE) is a very dynamic part of the ovary with an important role in ovulation during reproductive period. It represents a source of germ cells in fetal period but data concerning that role of OSE in postnatal period are contradictory. Though OSE is a subject of research in past few years, it’s ultrastructure in newborn rats has not been studied yet.
The aim of the study was to define ultrastructure features of OSE in rats in the first 24h hours after birth.
Six newborn rats within first 24 hours after birth were euthanized. Ovaries were fixed in 4% glutaraldehyde, post-fixed in 1% osmium tetroxide, routinely dehydrated and embedded in araldite. Semi-thin sections were stained with toluidine blue and used to select areas of interest. Ultra-thin sections of selected areas were mounted on copper grids and examined on a Philips CM12 transmission electron microscope. Descriptive ultrastrustructure analysis was performed. OSE in newborn rats was mostly single lay...ered and sporadically pseudostratified, consisted from mostly cuboidal and columnar cells. These cells were with centrally positioned nucleus, mostly with irregularly dispersed chromatin. Microvili and cytoplasmic projections extended to peritoneal cavity from the surface of the cells. No continuous tunica albuginea was found, but in places, groups of fibroblast-like cells were present, indicating the onset of its formation. Numerous oogonia and oocytes were identified between OSE cells or up to the basal part of them. This may indicate that extrusion (the release of germ cells into a peritoneal cavity) may be an important way of germ cells elimination.
Early postnatal period in rat ovaries characterize with massive cell death of oogonia and oocytes, mostly by apoptosis and autophagy, but further investigation might reveal the participation of this way of germ cells elimination realizing the importance of appropriate course of cell death process in terms of reproductive health.
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7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb, 2017, 153-153Publisher:
- Faculty of Veterinary Medicine, University of Zagreb
Funding / projects:
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-MESTD-Basic Research (BR or ON)-175061)
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Fakultet veterinarske medicineTY - CONF AU - Danilović Luković, Jelena AU - Korać, Aleksandra AU - Milošević, Ivan AU - Lužajić, Tijana AU - Kovačević Filipović, Milica AU - Radovanović, Anita PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2599 AB - Ovarian surface epithelium (OSE) is a very dynamic part of the ovary with an important role in ovulation during reproductive period. It represents a source of germ cells in fetal period but data concerning that role of OSE in postnatal period are contradictory. Though OSE is a subject of research in past few years, it’s ultrastructure in newborn rats has not been studied yet. The aim of the study was to define ultrastructure features of OSE in rats in the first 24h hours after birth. Six newborn rats within first 24 hours after birth were euthanized. Ovaries were fixed in 4% glutaraldehyde, post-fixed in 1% osmium tetroxide, routinely dehydrated and embedded in araldite. Semi-thin sections were stained with toluidine blue and used to select areas of interest. Ultra-thin sections of selected areas were mounted on copper grids and examined on a Philips CM12 transmission electron microscope. Descriptive ultrastrustructure analysis was performed. OSE in newborn rats was mostly single layered and sporadically pseudostratified, consisted from mostly cuboidal and columnar cells. These cells were with centrally positioned nucleus, mostly with irregularly dispersed chromatin. Microvili and cytoplasmic projections extended to peritoneal cavity from the surface of the cells. No continuous tunica albuginea was found, but in places, groups of fibroblast-like cells were present, indicating the onset of its formation. Numerous oogonia and oocytes were identified between OSE cells or up to the basal part of them. This may indicate that extrusion (the release of germ cells into a peritoneal cavity) may be an important way of germ cells elimination. Early postnatal period in rat ovaries characterize with massive cell death of oogonia and oocytes, mostly by apoptosis and autophagy, but further investigation might reveal the participation of this way of germ cells elimination realizing the importance of appropriate course of cell death process in terms of reproductive health. PB - Faculty of Veterinary Medicine, University of Zagreb C3 - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb T1 - Ovarian surface epithelium in newborn rats: germ cells warehouse SP - 153 EP - 153 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2599 ER -
@conference{ author = "Danilović Luković, Jelena and Korać, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Kovačević Filipović, Milica and Radovanović, Anita", year = "2017", abstract = "Ovarian surface epithelium (OSE) is a very dynamic part of the ovary with an important role in ovulation during reproductive period. It represents a source of germ cells in fetal period but data concerning that role of OSE in postnatal period are contradictory. Though OSE is a subject of research in past few years, it’s ultrastructure in newborn rats has not been studied yet. The aim of the study was to define ultrastructure features of OSE in rats in the first 24h hours after birth. Six newborn rats within first 24 hours after birth were euthanized. Ovaries were fixed in 4% glutaraldehyde, post-fixed in 1% osmium tetroxide, routinely dehydrated and embedded in araldite. Semi-thin sections were stained with toluidine blue and used to select areas of interest. Ultra-thin sections of selected areas were mounted on copper grids and examined on a Philips CM12 transmission electron microscope. Descriptive ultrastrustructure analysis was performed. OSE in newborn rats was mostly single layered and sporadically pseudostratified, consisted from mostly cuboidal and columnar cells. These cells were with centrally positioned nucleus, mostly with irregularly dispersed chromatin. Microvili and cytoplasmic projections extended to peritoneal cavity from the surface of the cells. No continuous tunica albuginea was found, but in places, groups of fibroblast-like cells were present, indicating the onset of its formation. Numerous oogonia and oocytes were identified between OSE cells or up to the basal part of them. This may indicate that extrusion (the release of germ cells into a peritoneal cavity) may be an important way of germ cells elimination. Early postnatal period in rat ovaries characterize with massive cell death of oogonia and oocytes, mostly by apoptosis and autophagy, but further investigation might reveal the participation of this way of germ cells elimination realizing the importance of appropriate course of cell death process in terms of reproductive health.", publisher = "Faculty of Veterinary Medicine, University of Zagreb", journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb", title = "Ovarian surface epithelium in newborn rats: germ cells warehouse", pages = "153-153", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2599" }
Danilović Luković, J., Korać, A., Milošević, I., Lužajić, T., Kovačević Filipović, M.,& Radovanović, A.. (2017). Ovarian surface epithelium in newborn rats: germ cells warehouse. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb Faculty of Veterinary Medicine, University of Zagreb., 153-153. https://hdl.handle.net/21.15107/rcub_veterinar_2599
Danilović Luković J, Korać A, Milošević I, Lužajić T, Kovačević Filipović M, Radovanović A. Ovarian surface epithelium in newborn rats: germ cells warehouse. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:153-153. https://hdl.handle.net/21.15107/rcub_veterinar_2599 .
Danilović Luković, Jelena, Korać, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Kovačević Filipović, Milica, Radovanović, Anita, "Ovarian surface epithelium in newborn rats: germ cells warehouse" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):153-153, https://hdl.handle.net/21.15107/rcub_veterinar_2599 .