Analysis of the CHD gene in birds as a molecular marker for sex determination
Analiza CHD gena ptica kao molekularnog markera za determinaciju pola
2014
Authors
Vučićević, MilošContributors
Stevanović, JevrosimaResanović, Radmila
Stanimirović, Zoran
Palić, Todor
Šekler, Milanko
Doctoral thesis (Published version)
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Sex determination in birds is extremely difficult, primarily due to the fact that more than 50% of bird species are monomorphic. Many traditional methods that have been used before defining molecular genetics methods have their biggest lack as their main characteristic – the unreliability. The results obtained using different methods should be primarily reliable and analysis should be fast, secure and cost-effective. Highly conserved CHD gene has been discovered in 1995 on the W chromosome, and in 1997 on the Z chromosome. The difference in the intron length between the two chromosomes allows sex determination after the PCR reaction. Blood, feathers, buccal swabs, faeces and formalin fixed paraffin embedded tissue samples were used for analysis. We compared the success of the reaction depending on which sample was used, also we compared the success of the reactions when used ordinary polymerases and polymerase designed to be resistant to PCR inhibitors. Using PCR and set of primers 255...0F/2718R we have examined the possibility of using CHD gene as molecular markers for sex determination of 550 individuals originating from 83 species of birds widely distributed along the phylogenetic tree. Sex was successfully determined in 74 bird species. In 26 of those it was carried out for the first time using molecular markers and PCR...
Određivanje pola kod ptica je izuzetno teško, prvenstveno zbog činjenice da je preko 50% vrsta monomorfno. Pre definisanja molekularno genetičkih metoda, za determinaciju pola kod ptica koristile su se mnoge tradicionalne metode čija je glavna karakteristika, a ujedno i najveća mana – nepouzdanost. Rezultati koji se dobijaju primenom različitih metoda moraju biti pre svega pouzdani, a analize ekonomične, brze i bezbedne. Visokokonzervisani CHD gen je otkriven 1995. godine na W hromozomu ptica, dok je na Z hromozomu otkriven 1997. godine. Postojanje razlike u dužini introna između Z i W hromozoma omogućava razlikovanje polova nakon PCR reakcije. U radu su kao uzorci korišćeni perje, krv, bris usne duplje, feces i parafinski tkivni isečci različitih tkiva. Poređena je uspešnost reakcija u zavisnosti od toga koji je uzorak korišćen, kao i uspešnost reakcija kada se koristila obična polimeraza i polimeraza specijalno dizajnirana da bude otporna na inhibitore PCR reakcije. Korišćenjem PCR t...ehnike i seta prajmera 2550F/2718R ispitana je mogućnost primene CHD gena kao molekularnog markera za određivanje pola kod 550 jedinki poreklom od 83 vrsta ptica široko distribuiranih duž filogenetskog stabla...
Keywords:
CHD gene / Birds / Sex determination using molecular genetic methods / CHD ген / Ptice / Molekularno genetičke metode određivanja polaSource:
2014Publisher:
- Univerzitet u Beogradu, Fakultet veterinarske medicine
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
URI
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45327375http://nardus.mpn.gov.rs/123456789/2990
https://vet-erinar.vet.bg.ac.rs/handle/123456789/25
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Fakultet veterinarske medicineTY - THES AU - Vučićević, Miloš PY - 2014 UR - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=45327375 UR - http://nardus.mpn.gov.rs/123456789/2990 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/25 AB - Sex determination in birds is extremely difficult, primarily due to the fact that more than 50% of bird species are monomorphic. Many traditional methods that have been used before defining molecular genetics methods have their biggest lack as their main characteristic – the unreliability. The results obtained using different methods should be primarily reliable and analysis should be fast, secure and cost-effective. Highly conserved CHD gene has been discovered in 1995 on the W chromosome, and in 1997 on the Z chromosome. The difference in the intron length between the two chromosomes allows sex determination after the PCR reaction. Blood, feathers, buccal swabs, faeces and formalin fixed paraffin embedded tissue samples were used for analysis. We compared the success of the reaction depending on which sample was used, also we compared the success of the reactions when used ordinary polymerases and polymerase designed to be resistant to PCR inhibitors. Using PCR and set of primers 2550F/2718R we have examined the possibility of using CHD gene as molecular markers for sex determination of 550 individuals originating from 83 species of birds widely distributed along the phylogenetic tree. Sex was successfully determined in 74 bird species. In 26 of those it was carried out for the first time using molecular markers and PCR... AB - Određivanje pola kod ptica je izuzetno teško, prvenstveno zbog činjenice da je preko 50% vrsta monomorfno. Pre definisanja molekularno genetičkih metoda, za determinaciju pola kod ptica koristile su se mnoge tradicionalne metode čija je glavna karakteristika, a ujedno i najveća mana – nepouzdanost. Rezultati koji se dobijaju primenom različitih metoda moraju biti pre svega pouzdani, a analize ekonomične, brze i bezbedne. Visokokonzervisani CHD gen je otkriven 1995. godine na W hromozomu ptica, dok je na Z hromozomu otkriven 1997. godine. Postojanje razlike u dužini introna između Z i W hromozoma omogućava razlikovanje polova nakon PCR reakcije. U radu su kao uzorci korišćeni perje, krv, bris usne duplje, feces i parafinski tkivni isečci različitih tkiva. Poređena je uspešnost reakcija u zavisnosti od toga koji je uzorak korišćen, kao i uspešnost reakcija kada se koristila obična polimeraza i polimeraza specijalno dizajnirana da bude otporna na inhibitore PCR reakcije. Korišćenjem PCR tehnike i seta prajmera 2550F/2718R ispitana je mogućnost primene CHD gena kao molekularnog markera za određivanje pola kod 550 jedinki poreklom od 83 vrsta ptica široko distribuiranih duž filogenetskog stabla... PB - Univerzitet u Beogradu, Fakultet veterinarske medicine T1 - Analysis of the CHD gene in birds as a molecular marker for sex determination T1 - Analiza CHD gena ptica kao molekularnog markera za determinaciju pola UR - https://hdl.handle.net/21.15107/rcub_nardus_2990 ER -
@phdthesis{ author = "Vučićević, Miloš", year = "2014", abstract = "Sex determination in birds is extremely difficult, primarily due to the fact that more than 50% of bird species are monomorphic. Many traditional methods that have been used before defining molecular genetics methods have their biggest lack as their main characteristic – the unreliability. The results obtained using different methods should be primarily reliable and analysis should be fast, secure and cost-effective. Highly conserved CHD gene has been discovered in 1995 on the W chromosome, and in 1997 on the Z chromosome. The difference in the intron length between the two chromosomes allows sex determination after the PCR reaction. Blood, feathers, buccal swabs, faeces and formalin fixed paraffin embedded tissue samples were used for analysis. We compared the success of the reaction depending on which sample was used, also we compared the success of the reactions when used ordinary polymerases and polymerase designed to be resistant to PCR inhibitors. Using PCR and set of primers 2550F/2718R we have examined the possibility of using CHD gene as molecular markers for sex determination of 550 individuals originating from 83 species of birds widely distributed along the phylogenetic tree. Sex was successfully determined in 74 bird species. In 26 of those it was carried out for the first time using molecular markers and PCR..., Određivanje pola kod ptica je izuzetno teško, prvenstveno zbog činjenice da je preko 50% vrsta monomorfno. Pre definisanja molekularno genetičkih metoda, za determinaciju pola kod ptica koristile su se mnoge tradicionalne metode čija je glavna karakteristika, a ujedno i najveća mana – nepouzdanost. Rezultati koji se dobijaju primenom različitih metoda moraju biti pre svega pouzdani, a analize ekonomične, brze i bezbedne. Visokokonzervisani CHD gen je otkriven 1995. godine na W hromozomu ptica, dok je na Z hromozomu otkriven 1997. godine. Postojanje razlike u dužini introna između Z i W hromozoma omogućava razlikovanje polova nakon PCR reakcije. U radu su kao uzorci korišćeni perje, krv, bris usne duplje, feces i parafinski tkivni isečci različitih tkiva. Poređena je uspešnost reakcija u zavisnosti od toga koji je uzorak korišćen, kao i uspešnost reakcija kada se koristila obična polimeraza i polimeraza specijalno dizajnirana da bude otporna na inhibitore PCR reakcije. Korišćenjem PCR tehnike i seta prajmera 2550F/2718R ispitana je mogućnost primene CHD gena kao molekularnog markera za određivanje pola kod 550 jedinki poreklom od 83 vrsta ptica široko distribuiranih duž filogenetskog stabla...", publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine", title = "Analysis of the CHD gene in birds as a molecular marker for sex determination, Analiza CHD gena ptica kao molekularnog markera za determinaciju pola", url = "https://hdl.handle.net/21.15107/rcub_nardus_2990" }
Vučićević, M.. (2014). Analysis of the CHD gene in birds as a molecular marker for sex determination. Univerzitet u Beogradu, Fakultet veterinarske medicine.. https://hdl.handle.net/21.15107/rcub_nardus_2990
Vučićević M. Analysis of the CHD gene in birds as a molecular marker for sex determination. 2014;. https://hdl.handle.net/21.15107/rcub_nardus_2990 .
Vučićević, Miloš, "Analysis of the CHD gene in birds as a molecular marker for sex determination" (2014), https://hdl.handle.net/21.15107/rcub_nardus_2990 .