Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens
Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan
Authors
Maletić, JelenaKureljušić, Branislav
Spalević, Ljiljana
Vučićević, Ivana
Veljović, Ljubiša
Milovanović, Bojan
Milićević, Vesna
Conference object (Published version)
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Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many
reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian
leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders
in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia,
apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching
20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis,
splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart.
Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while
granulo...matous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and
arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces
revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of
oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis
virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as
molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus.
Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća
opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i
virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski
gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane
simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće
promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju,
višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene,
proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin
srebrno bojenje po Gomoriju otkrilo je sept...irane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim
granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog
crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove
bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i
molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.
Keywords:
avian leucosis virus / Banat Naked Neck hens / Marek’s disease virus / virus ptičje leukoze / kokoš pasmine Banatski gološijan / virus Marekove bolestSource:
XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022, 2022, 210-217Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200030 (Scientific Veterinary Institute of Serbia, Belgrade) (RS-MESTD-inst-2020-200030)
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- Proceedings
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Fakultet veterinarske medicineTY - CONF AU - Maletić, Jelena AU - Kureljušić, Branislav AU - Spalević, Ljiljana AU - Vučićević, Ivana AU - Veljović, Ljubiša AU - Milovanović, Bojan AU - Milićević, Vesna PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2866 AB - Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia, apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching 20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis, splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart. Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus. AB - Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju, višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene, proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze. C3 - XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022 T1 - Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens T1 - Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan SP - 210 EP - 217 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2866 ER -
@conference{ author = "Maletić, Jelena and Kureljušić, Branislav and Spalević, Ljiljana and Vučićević, Ivana and Veljović, Ljubiša and Milovanović, Bojan and Milićević, Vesna", year = "2022", abstract = "Marek’s disease virus and avian leucosis virus are causes of contagious, immunosuppressive, and oncogenic diseases. Many reports have described single virus infection, but detailed description of co-infection with Marek’s disease virus and avian leucosis virus has been provided in a few previous reports. The aim of this study was to determine the cause of health disorders in a flock of the Banat Naked Neck hens at the age of 11 weeks. Clinical examination of the flock showed signs of cachexia, apathy, dropped wings, nervous symptoms in the form of bilateral leg paresis, sporadic diarrhoea, and the mortality reaching 20%. Post-mortem examinations showed the following changes: chronic pseudomembranous typhlitis, intestinal ascaridiasis, splenomegaly, granulomatous pneumonia, multiple yellowish caseous foci in the liver, and white tumour masses in the heart. Histopathological examination of the spleen, proventriculus and heart tissue revealed polymorphic infiltrates, while granulomatous inflammation was observed in the lung and liver. Gomori methenamine silver stain method revealed septate and arborized hyphae resembling the Aspergillus spp. fungi in the liver and lung granulomas. Bacteriological examination of faeces revealed the presence of Escherichia coli, and parasitological examination of the small intestine determined the presence of oocyst of Eimeria acervulina and Eimeria tenella in the caecum. The genomes of Marek's disease virus and avian leucosis virus were determined by polymerase chain reaction in tissue samples. In this case, specific pathological lesions, as well as molecular finding confirmed co-infection with Marek’s disease virus and avian leucosis virus., Virus Marekove bolesti i virus ptičje leukoze uzrokuju zarazne, imunosupresivne i onkogene bolesti. Mnoga izvješća opisuju infekciju pojedinim virusom, ali tek mali broj izvješća podrobnije opisuje koinfekciju virusom Marekove bolesti i virusom ptičje leukoze. Cilj ovog istraživanja bio je utvrditi uzrok zdravstvenih poremećaja u jatu kokoši pasmine Banatski gološijan u dobi od 11 tjedana. Klinički pregled jata pokazao je znakove kaheksije, apatije, spuštena krila, živčane simptome u obliku pareze nogu, sporadični proljev te 20%-tnu smrtnost. Postmortem pregledi pokazali su sljedeće promjene: kronični pseudomembranski tiflitis, crijevnu askaridijazu, splenomegaliju, granulomatoznu pneumoniju, višestruka žućkasta sirasta žarišta u jetri i bijele tumorske tvorbe u srcu. Patohistološki pregled tkivnih uzoraka slezene, proventrikla i srca otkrio je polimorfne infiltrate, dok je u plućima i jetri zabilježena granulomatozna upala. Metenamin srebrno bojenje po Gomoriju otkrilo je septirane i arborizirane hife nalik gljivama Aspergillus spp. u jetrenim i plućnim granulomima. Bakteriološke pretrage fecesa pokazale su prisutnost Escherichia coli, dok je parazitološka pretraga tankog crijeva utvrdila prisutnost oocita Eimeria acervulina te Eimeria tenella u cekumu. PCR-om su genomi virusa Marekove bolesti i virusa ptičje leukoze utvrđeni u tkivnim uzorcima. U ovom istraživanju su specifične patološke promjene i molekularni nalazi potvrdili koinfekciju virusom Marekove bolesti i virusom ptičje leukoze.", journal = "XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022", title = "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens, Koinfekcija virusom Marekove bolesti i virusom ptičje leukoze kod kokoši pasmine banatski gološijan", pages = "210-217", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2866" }
Maletić, J., Kureljušić, B., Spalević, L., Vučićević, I., Veljović, L., Milovanović, B.,& Milićević, V.. (2022). Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022, 210-217. https://hdl.handle.net/21.15107/rcub_veterinar_2866
Maletić J, Kureljušić B, Spalević L, Vučićević I, Veljović L, Milovanović B, Milićević V. Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens. in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022. 2022;:210-217. https://hdl.handle.net/21.15107/rcub_veterinar_2866 .
Maletić, Jelena, Kureljušić, Branislav, Spalević, Ljiljana, Vučićević, Ivana, Veljović, Ljubiša, Milovanović, Bojan, Milićević, Vesna, "Co-infection with Marek’s disease virus and avian leukosis virus in the banat naked neck hens" in XIV Symposium poultry days 2022 With International Participation, Croatia, Poreč, May 11‐14, 2022 (2022):210-217, https://hdl.handle.net/21.15107/rcub_veterinar_2866 .
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