Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries
Autori
Cutarelli, AnnaDe Falco, Francesca
Cuccaro, Bianca
Milićević, Vesna
Kureljušić, Branislav
Bojkovski, Jovan
Cerino, Pellegrino
Perillo, Antonella
Marica, Raluca
Catoi, Cornel
Roperto, Sante
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected
and quantified for the first time using droplet digital polymerase chain reaction
(ddPCR)
blood samples of 374 clinically healthy goats from farms located
in
in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA in 78 of the
374 examined samples, indicating that
~21% of the goats harbored circulating
papillomavirus DNA. In particular, in Italian goat farms, ChPV genotypes were
detected and quantified in 58 of 157 blood samples (~37%), 11 of 117 samples
from Serbian farms (~9.4%), and 9 of 100 from Romanian blood samples (9%).
Blood samples fromItalian goat farms showed a high prevalence of ChPV1, which
was detected in 45 samples (28.6%). The ChPV2 genotype was detected in 13
samples (~8.3%). Therefore, significant dierences in prevalence and genotype
distributions were observed. On Serbian and Romanian farms, no significan
dierences were observed in the genotype prevalence of ChPVs.... Molecular
findings are consistent with ChPV prevalence, characterized by a territorial
distribution similar to that of papillomaviruses in other mammalian species.
Furthermore, this study showed that ddPCR is a very sensitive and accurate
assay for ChPV detection and quantification. The ddPCR may be the molecular
diagnostic tool of choice, ultimately providing useful insights into the molecular
epidemiology and field surveillance of ChPV.
Ključne reči:
blood / caprine papillomaviruses / ChPVs / droplet digital PCR / goat / liquid biopsy / molecular epidemiologyIzvor:
Frontiers in Veterinary Science, 2023, 10, 1213150-Izdavač:
- Frontiers Media
Finansiranje / projekti:
- Istituto Zooprofilattic Sperimentale del Mezzogiorno
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Cutarelli, Anna AU - De Falco, Francesca AU - Cuccaro, Bianca AU - Milićević, Vesna AU - Kureljušić, Branislav AU - Bojkovski, Jovan AU - Cerino, Pellegrino AU - Perillo, Antonella AU - Marica, Raluca AU - Catoi, Cornel AU - Roperto, Sante PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3147 AB - Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected and quantified for the first time using droplet digital polymerase chain reaction (ddPCR) blood samples of 374 clinically healthy goats from farms located in in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA in 78 of the 374 examined samples, indicating that ~21% of the goats harbored circulating papillomavirus DNA. In particular, in Italian goat farms, ChPV genotypes were detected and quantified in 58 of 157 blood samples (~37%), 11 of 117 samples from Serbian farms (~9.4%), and 9 of 100 from Romanian blood samples (9%). Blood samples fromItalian goat farms showed a high prevalence of ChPV1, which was detected in 45 samples (28.6%). The ChPV2 genotype was detected in 13 samples (~8.3%). Therefore, significant dierences in prevalence and genotype distributions were observed. On Serbian and Romanian farms, no significan dierences were observed in the genotype prevalence of ChPVs. Molecular findings are consistent with ChPV prevalence, characterized by a territorial distribution similar to that of papillomaviruses in other mammalian species. Furthermore, this study showed that ddPCR is a very sensitive and accurate assay for ChPV detection and quantification. The ddPCR may be the molecular diagnostic tool of choice, ultimately providing useful insights into the molecular epidemiology and field surveillance of ChPV. PB - Frontiers Media T2 - Frontiers in Veterinary Science T1 - Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries VL - 10 SP - 1213150 DO - 10.3389/fvets.2023.1213150 ER -
@article{ author = "Cutarelli, Anna and De Falco, Francesca and Cuccaro, Bianca and Milićević, Vesna and Kureljušić, Branislav and Bojkovski, Jovan and Cerino, Pellegrino and Perillo, Antonella and Marica, Raluca and Catoi, Cornel and Roperto, Sante", year = "2023", abstract = "Caprine papillomaviruses (ChPVs, Capra hircus papillomaviruses) were detected and quantified for the first time using droplet digital polymerase chain reaction (ddPCR) blood samples of 374 clinically healthy goats from farms located in in Italy, Romania, and Serbia. Overall, ddPCR revealed ChPV DNA in 78 of the 374 examined samples, indicating that ~21% of the goats harbored circulating papillomavirus DNA. In particular, in Italian goat farms, ChPV genotypes were detected and quantified in 58 of 157 blood samples (~37%), 11 of 117 samples from Serbian farms (~9.4%), and 9 of 100 from Romanian blood samples (9%). Blood samples fromItalian goat farms showed a high prevalence of ChPV1, which was detected in 45 samples (28.6%). The ChPV2 genotype was detected in 13 samples (~8.3%). Therefore, significant dierences in prevalence and genotype distributions were observed. On Serbian and Romanian farms, no significan dierences were observed in the genotype prevalence of ChPVs. Molecular findings are consistent with ChPV prevalence, characterized by a territorial distribution similar to that of papillomaviruses in other mammalian species. Furthermore, this study showed that ddPCR is a very sensitive and accurate assay for ChPV detection and quantification. The ddPCR may be the molecular diagnostic tool of choice, ultimately providing useful insights into the molecular epidemiology and field surveillance of ChPV.", publisher = "Frontiers Media", journal = "Frontiers in Veterinary Science", title = "Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries", volume = "10", pages = "1213150", doi = "10.3389/fvets.2023.1213150" }
Cutarelli, A., De Falco, F., Cuccaro, B., Milićević, V., Kureljušić, B., Bojkovski, J., Cerino, P., Perillo, A., Marica, R., Catoi, C.,& Roperto, S.. (2023). Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries. in Frontiers in Veterinary Science Frontiers Media., 10, 1213150. https://doi.org/10.3389/fvets.2023.1213150
Cutarelli A, De Falco F, Cuccaro B, Milićević V, Kureljušić B, Bojkovski J, Cerino P, Perillo A, Marica R, Catoi C, Roperto S. Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries. in Frontiers in Veterinary Science. 2023;10:1213150. doi:10.3389/fvets.2023.1213150 .
Cutarelli, Anna, De Falco, Francesca, Cuccaro, Bianca, Milićević, Vesna, Kureljušić, Branislav, Bojkovski, Jovan, Cerino, Pellegrino, Perillo, Antonella, Marica, Raluca, Catoi, Cornel, Roperto, Sante, "Prevalence and genotype distribution of caprine papillomavirus in peripheral blood of healthy goats in farms from three European countries" in Frontiers in Veterinary Science, 10 (2023):1213150, https://doi.org/10.3389/fvets.2023.1213150 . .