Uticaj humanog horiogonadotropina na neurone nukleusa medijalisa amigdale kod ženki pacova višekratno tretiranih u vreme polne zrelosti

Abstract

Humani horiogonadotropin (hCG) pripada familiji glikoproteinskih hormona, molekularne mase 38 kD /Mr/. S obzirom na to da su naša ranija proučavanja pokazala da su neuroni oba filogenetska dela amigdale (AMY), corticomedialis (CM) i basolateralis (BL), osetljivi na egzogeno uneti hCG, u sadašnjem istraživanju, primenom morfometrijskih analiza, želeli smo da utvrdimo da li i kako hCG deluje na neurone medijalnog amigdaloidnog nukleusa (NM AMY). Ženke pacova tretirane su jednom dozom 5 IU hCG, višekratno, u vreme polne zrelosti, od 46. do 68. dana života, a žrtvovane su u etarskoj narkozi 69. dana starosti. Mozak je izolovan, fiksiran u Bouin soluciji i ukalupljen u parafin. Preparati su serijski sečeni sa debljinom reza od 5 µm i obojeni H&E, po Bodianu i Biaschowskom. Morfometrijskom analizom, uz upotrebu Waibel-ovog mnogonamenskog testnog sistema, ispitivan je volumen jedara neurona (V) NM AMY i broj neurona na jedinicu površine (NA). Statistička značajnost je određivana primenom Studentovog t-testa. Morfometrijska analiza volumena jedara neurona (V) NM AMY kontrolne grupe ženki pacova žrtvovanih 69. dana starosti pokazala je da volumen (V) iznosi 500,51 (µm3), a broj neurona na jedinicu površine (NA) 1095,5 (mm2). Kod ženki pacova koje su višekratno tretirane sa 5 IU hCG u periodu od 46. do 68. dana starosti, volumen jedara neurona iznosi 637,79 (µm3), a broj neurona na jedinicu površine (NA) 641,2 (mm2). Statističkom analizom ustanovili smo da postoji povećanje volumena jedara neurona NM AMY tretiranih ženki pacova (p<0.01) i statističko smanjenje broja neurona (NA) na jedinicu površine (p<0.01). Analiza rezultata ženki pacova višekratno tretiranih sa hCG, koje su žrtvovane 69. dana života, u polno zrelom periodu, pokazala je da hCG ispoljava efekat povećanja volumena jedara neurona (V) i smanjenja broja neurona na jedinicu površine (NA).

Human chorionic gonadotropin (hCG) belongs to family of glycoprotein hormones with molecular mass of 38kD /Mr/. Considering that our earlier investigation showed that the neurons of both phylogenetical amygdala (AMY) parts, AMY corticomedialis and AMY basolateralis were sensitive of exogenous hCG, in the present investigation using morphometric analysis, we wanted to establish if and how, hCG is acting on neurons of medial amygdaloid nucleus. The female rats were treated with single dose of 5 IU hCG repeatedly from 46. to 68. day, in their mature period of life, and sacrificed in ether narcosis on the 69. day of life. The brains were isolated, fixed in Bouin solution and formed into paraffin. The preparations, 5 µm serial cutted, were stained with H&E, and method according to Bodian and Biaschowsky. Using morphometric analyses, with the Weibel multipurpose test system, we investigate the volume of neuronal nuclei /V/ NM AMY and the number of neurons (NA) per square unit (mm2). Statistical significance was tested with Student’s T-test. In the control female rats group (sacrificed on the 69. day of life), the morphometric analysis of V NM AMY showed that the V was 500,51 µm3 and the number of neurons (NA) 1009,5 mm2. In treated female rats group (repeatedly treated with 5 IU hCG from 46. to 68. day of life), the volume of neuronal nuclei was 637,79 µm3 and the number of neurons (NA) per square unit was 641,2 mm2. With statistical analysis in treated female rats we establish the increase in volume of neuronal nuclei of NM AMY (p<0.01) and statistical decrease (p<0.01) in number of neurons (NA). Analyzing the results of female rats repeatedly treated with hCG and sacrificed on 69. day of life (mature rats) showed, that hCG demonstrates the increase in volume neuronal nuclei /V/ and decrease in number of neurons (NA) per square unit.