Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs
Autori
Ilić, MilicaIvković, Maša
Radaković, Milena
Spariosu, Kristina
Andrić, Nenad
Kovačević Filipović, Milica
Beletić, Anđelo
Francuski Andrić, Jelena
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Red blood cell osmotic fragility (RBCOF) refers to susceptibility to hemolysis in
vivo and in vitro. An increase in mean cellular hemoglobin concentration (MCHC)
also reflects in vivo and in vitro hemolysis. Common inflammatory infectious
diseases in dogs are often characterized by an increase in MCHC; however, it is
not known if there is a concomitant increase in RBCOF. The objective of this
study was to determine RBCOF in dogs with babesiosis, dirofilariosis,
parvovirosis, pyometra, and canine distemper and to investigate whether RBCOF
correlates with routine hematologic and biochemical parameters and lipoprotein
fractions. The diagnosis of canine infectious disease was made and complete
blood count, serum biochemical profiles, and lipoprotein electrophoresis were
determined according to current institutional protocols. RBCOF of healthy
control (n=8) and infected dogs (n=73) was measured with a phosphate-buffered
saline dilution test, and mean corpuscular fragility (MCF) ...was calculated. MCF
was increased in dirofilariosis, pyometra, and distemper; MCHC was increased
in all infected groups; HDL was decreased in babesiosis, pyometra, and
distemper compared with healthy controls. However, no correlation was found
between these parameters. On the other hand, hematological and biochemical
parameters, that are highly predictive of specific infections, correlated with MCF:
ALP for babesiosis (r=0.415, P=0.035), urea (r=-0.488, P=0.034) for
dirofilariosis, and leukocyte count (r=-0.820, P=0.046) for pyometra. Although
elevated MCHC levels in all samples from infected dogs indicate hemolysis,
RBCOF was elevated only in dirofilariosis, pyometra, and canine distemper,
leading to the conclusion that these apparently related phenomena are controlled
by different mechanisms.
Ključne reči:
Dog / Erythrocyte osmotic fragility / Infection / LipoproteinsIzvor:
Pakistan Veterinary Journal, 2023, 43, 3, 463-469Izdavač:
- Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200143 (Univerzitet u Beogradu, Fakultet veterinarske medicine) (RS-MESTD-inst-2020-200143)
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Ilić, Milica AU - Ivković, Maša AU - Radaković, Milena AU - Spariosu, Kristina AU - Andrić, Nenad AU - Kovačević Filipović, Milica AU - Beletić, Anđelo AU - Francuski Andrić, Jelena PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3254 AB - Red blood cell osmotic fragility (RBCOF) refers to susceptibility to hemolysis in vivo and in vitro. An increase in mean cellular hemoglobin concentration (MCHC) also reflects in vivo and in vitro hemolysis. Common inflammatory infectious diseases in dogs are often characterized by an increase in MCHC; however, it is not known if there is a concomitant increase in RBCOF. The objective of this study was to determine RBCOF in dogs with babesiosis, dirofilariosis, parvovirosis, pyometra, and canine distemper and to investigate whether RBCOF correlates with routine hematologic and biochemical parameters and lipoprotein fractions. The diagnosis of canine infectious disease was made and complete blood count, serum biochemical profiles, and lipoprotein electrophoresis were determined according to current institutional protocols. RBCOF of healthy control (n=8) and infected dogs (n=73) was measured with a phosphate-buffered saline dilution test, and mean corpuscular fragility (MCF) was calculated. MCF was increased in dirofilariosis, pyometra, and distemper; MCHC was increased in all infected groups; HDL was decreased in babesiosis, pyometra, and distemper compared with healthy controls. However, no correlation was found between these parameters. On the other hand, hematological and biochemical parameters, that are highly predictive of specific infections, correlated with MCF: ALP for babesiosis (r=0.415, P=0.035), urea (r=-0.488, P=0.034) for dirofilariosis, and leukocyte count (r=-0.820, P=0.046) for pyometra. Although elevated MCHC levels in all samples from infected dogs indicate hemolysis, RBCOF was elevated only in dirofilariosis, pyometra, and canine distemper, leading to the conclusion that these apparently related phenomena are controlled by different mechanisms. PB - Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan T2 - Pakistan Veterinary Journal T1 - Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs VL - 43 IS - 3 SP - 463 EP - 469 DO - 10.29261/pakvetj/2023.048 ER -
@article{ author = "Ilić, Milica and Ivković, Maša and Radaković, Milena and Spariosu, Kristina and Andrić, Nenad and Kovačević Filipović, Milica and Beletić, Anđelo and Francuski Andrić, Jelena", year = "2023", abstract = "Red blood cell osmotic fragility (RBCOF) refers to susceptibility to hemolysis in vivo and in vitro. An increase in mean cellular hemoglobin concentration (MCHC) also reflects in vivo and in vitro hemolysis. Common inflammatory infectious diseases in dogs are often characterized by an increase in MCHC; however, it is not known if there is a concomitant increase in RBCOF. The objective of this study was to determine RBCOF in dogs with babesiosis, dirofilariosis, parvovirosis, pyometra, and canine distemper and to investigate whether RBCOF correlates with routine hematologic and biochemical parameters and lipoprotein fractions. The diagnosis of canine infectious disease was made and complete blood count, serum biochemical profiles, and lipoprotein electrophoresis were determined according to current institutional protocols. RBCOF of healthy control (n=8) and infected dogs (n=73) was measured with a phosphate-buffered saline dilution test, and mean corpuscular fragility (MCF) was calculated. MCF was increased in dirofilariosis, pyometra, and distemper; MCHC was increased in all infected groups; HDL was decreased in babesiosis, pyometra, and distemper compared with healthy controls. However, no correlation was found between these parameters. On the other hand, hematological and biochemical parameters, that are highly predictive of specific infections, correlated with MCF: ALP for babesiosis (r=0.415, P=0.035), urea (r=-0.488, P=0.034) for dirofilariosis, and leukocyte count (r=-0.820, P=0.046) for pyometra. Although elevated MCHC levels in all samples from infected dogs indicate hemolysis, RBCOF was elevated only in dirofilariosis, pyometra, and canine distemper, leading to the conclusion that these apparently related phenomena are controlled by different mechanisms.", publisher = "Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan", journal = "Pakistan Veterinary Journal", title = "Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs", volume = "43", number = "3", pages = "463-469", doi = "10.29261/pakvetj/2023.048" }
Ilić, M., Ivković, M., Radaković, M., Spariosu, K., Andrić, N., Kovačević Filipović, M., Beletić, A.,& Francuski Andrić, J.. (2023). Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs. in Pakistan Veterinary Journal Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan., 43(3), 463-469. https://doi.org/10.29261/pakvetj/2023.048
Ilić M, Ivković M, Radaković M, Spariosu K, Andrić N, Kovačević Filipović M, Beletić A, Francuski Andrić J. Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs. in Pakistan Veterinary Journal. 2023;43(3):463-469. doi:10.29261/pakvetj/2023.048 .
Ilić, Milica, Ivković, Maša, Radaković, Milena, Spariosu, Kristina, Andrić, Nenad, Kovačević Filipović, Milica, Beletić, Anđelo, Francuski Andrić, Jelena, "Association of increased osmotic fragility of red blood cells with common systemic inflammatory diseases in dogs" in Pakistan Veterinary Journal, 43, no. 3 (2023):463-469, https://doi.org/10.29261/pakvetj/2023.048 . .