In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice
In vivo ispitivanje klastogenog efekta levamizol hidrohlorida na ćelijama kostne srži miševa BALB/c soja
Апстракт
Cytotoxic and genotoxic examinations were performed of the effect of levamisole hydrochloride (2.2 mg/kg bm, 4.4 mg/kg bm, LD50-25% mg/kg bm and LD50-75% mg/kg bm) on bone marrow cells of mice of the BALB/c strain. The effect of levamisole hydrochloride on kinetics of the cellular cycle and the appearance of structural and numerical changes in chromosomes of bone marrow cells were followed. The therapeutic dose of levamisole of 2.2 mg/kg bm showed the ability to increase the mitotic activity of the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, i.e. they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo showed the ability of inducing numeric (aneuloid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberratio...ns. On this basis, it can be concluded that the examined doses have a genotoxic effect.
Obavljena su citotoksična i genotoksična ispitivanja levamizol hidrohlorida (2.2 mg/kg, t.m., 4,4 mg/kg t.m, LD50-25% mg/kg t.m. i LD50-75% mg/kg t.m) na ćelijama kostne srži miša BALB/c soja. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavu strukturnih i numeričkih promena na hromosomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidije polipleidije) i strukturnih (lezije, prekidi i insercije) aberacija hromosoma. Na osnovu toga može da se zaključi da ispitivane doze imaju genotoksični efekat.
Кључне речи:
cytotoxicity / genotoxicity / mitotic index / structural aberrations / numerical aberrations / citotoksičnost / genotoksičnost / mitotski indeks / strukturne aberacije / numeričke aberacijeИзвор:
Veterinarski Glasnik, 2005, 59, 5-6, 549-556Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kulić, Milan AU - Stanimirović, Zoran AU - Ristić, Siniša AU - Marković, Biljana PY - 2005 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/358 AB - Cytotoxic and genotoxic examinations were performed of the effect of levamisole hydrochloride (2.2 mg/kg bm, 4.4 mg/kg bm, LD50-25% mg/kg bm and LD50-75% mg/kg bm) on bone marrow cells of mice of the BALB/c strain. The effect of levamisole hydrochloride on kinetics of the cellular cycle and the appearance of structural and numerical changes in chromosomes of bone marrow cells were followed. The therapeutic dose of levamisole of 2.2 mg/kg bm showed the ability to increase the mitotic activity of the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, i.e. they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo showed the ability of inducing numeric (aneuloid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. On this basis, it can be concluded that the examined doses have a genotoxic effect. AB - Obavljena su citotoksična i genotoksična ispitivanja levamizol hidrohlorida (2.2 mg/kg, t.m., 4,4 mg/kg t.m, LD50-25% mg/kg t.m. i LD50-75% mg/kg t.m) na ćelijama kostne srži miša BALB/c soja. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavu strukturnih i numeričkih promena na hromosomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidije polipleidije) i strukturnih (lezije, prekidi i insercije) aberacija hromosoma. Na osnovu toga može da se zaključi da ispitivane doze imaju genotoksični efekat. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice T1 - In vivo ispitivanje klastogenog efekta levamizol hidrohlorida na ćelijama kostne srži miševa BALB/c soja VL - 59 IS - 5-6 SP - 549 EP - 556 DO - 10.2298/VETGL0506549K ER -
@article{ author = "Kulić, Milan and Stanimirović, Zoran and Ristić, Siniša and Marković, Biljana", year = "2005", abstract = "Cytotoxic and genotoxic examinations were performed of the effect of levamisole hydrochloride (2.2 mg/kg bm, 4.4 mg/kg bm, LD50-25% mg/kg bm and LD50-75% mg/kg bm) on bone marrow cells of mice of the BALB/c strain. The effect of levamisole hydrochloride on kinetics of the cellular cycle and the appearance of structural and numerical changes in chromosomes of bone marrow cells were followed. The therapeutic dose of levamisole of 2.2 mg/kg bm showed the ability to increase the mitotic activity of the observed cells, thus confirming knowledge of the immunostimulative effect of this dose of the medicine under in vivo conditions. The other tested doses of levamisole in this experiment, observed in comparison with the control group, had an opposite effect, i.e. they caused a reduction in the mitotic activity of bone marrow cells. All the examined doses in vivo showed the ability of inducing numeric (aneuloid and polyploid) and structural (lesions, breaks and insertions) chromosomal aberrations. On this basis, it can be concluded that the examined doses have a genotoxic effect., Obavljena su citotoksična i genotoksična ispitivanja levamizol hidrohlorida (2.2 mg/kg, t.m., 4,4 mg/kg t.m, LD50-25% mg/kg t.m. i LD50-75% mg/kg t.m) na ćelijama kostne srži miša BALB/c soja. Praćen je uticaj levamizol hidrohlorida na kinetiku ćelijskog ciklusa i pojavu strukturnih i numeričkih promena na hromosomima u ćelijama kostne srži. Terapijska doza levamizola od 2,2 mg/kg t.m. pokazala je sposobnost povećanja mitotske aktivnosti posmatranih ćelija, tako potkrepljujući saznanja o imunostimulativnom efektu ove doze leka u in vivo uslovima. Ostale testirane doze levamizola u ovom eksperimentu, posmatrano u odnosu na kontrolnu grupu, imale su suprotan efekat, tj. uslovile su smanjenje mitotske aktivnosti ćelija kostne srži. Sve ispitivane doze in vivo pokazuju sposobnost indukcije numeričkih (aneuploidije polipleidije) i strukturnih (lezije, prekidi i insercije) aberacija hromosoma. Na osnovu toga može da se zaključi da ispitivane doze imaju genotoksični efekat.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice, In vivo ispitivanje klastogenog efekta levamizol hidrohlorida na ćelijama kostne srži miševa BALB/c soja", volume = "59", number = "5-6", pages = "549-556", doi = "10.2298/VETGL0506549K" }
Kulić, M., Stanimirović, Z., Ristić, S.,& Marković, B.. (2005). In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 549-556. https://doi.org/10.2298/VETGL0506549K
Kulić M, Stanimirović Z, Ristić S, Marković B. In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice. in Veterinarski Glasnik. 2005;59(5-6):549-556. doi:10.2298/VETGL0506549K .
Kulić, Milan, Stanimirović, Zoran, Ristić, Siniša, Marković, Biljana, "In vivo investigations of clastogenic effect of levamisole hydrochloride on bone marrow cells of BALB/c mice" in Veterinarski Glasnik, 59, no. 5-6 (2005):549-556, https://doi.org/10.2298/VETGL0506549K . .