Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes
2007
Autori
Đelić, NinoslavĐelić, Dijana
Spremo-Potparević, Biljana
Živković, Lada
Marković, Biljana
Lozanče, Olivera
Blagojević, Miloš
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage... or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.
Ključne reči:
thyroxine / human lymphocytes / chromosome aberrations / mitotic index / ROSIzvor:
Genetics and Molecular Biology, 2007, 30, 4, 1144-1149Izdavač:
- Soc Brasil Genetica, Ribeirao Pret
Finansiranje / projekti:
- Evaluacija dejstva hormona i citostatika prmenom citogenetičkih analiza i Komet testa (RS-MESTD-MPN2006-2010-143018)
DOI: 10.1590/S1415-47572007000600019
ISSN: 1415-4757
WoS: 000251771400019
Scopus: 2-s2.0-37649000465
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav AU - Đelić, Dijana AU - Spremo-Potparević, Biljana AU - Živković, Lada AU - Marković, Biljana AU - Lozanče, Olivera AU - Blagojević, Miloš PY - 2007 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/455 AB - Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species. PB - Soc Brasil Genetica, Ribeirao Pret T2 - Genetics and Molecular Biology T1 - Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes VL - 30 IS - 4 SP - 1144 EP - 1149 DO - 10.1590/S1415-47572007000600019 ER -
@article{ author = "Đelić, Ninoslav and Đelić, Dijana and Spremo-Potparević, Biljana and Živković, Lada and Marković, Biljana and Lozanče, Olivera and Blagojević, Miloš", year = "2007", abstract = "Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 mu M to 50 mu M of L-thyroxine for 24 h and 48 h, these concentrations being chosen because they had been used in previous studies of sister-chromatid exchange and micronuclei frequency. Under our experimental conditions thyroxine did not induced any statistically significant increase in chromosome breakage or aberrations. This lack of clastogenic effects is in contrast to the reported comet assay results obtained using purified lymphocytes, possibly because whole-blood cultures contain catalase and glutathione peroxidase capable of reducing the effects of reactive oxygen species.", publisher = "Soc Brasil Genetica, Ribeirao Pret", journal = "Genetics and Molecular Biology", title = "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes", volume = "30", number = "4", pages = "1144-1149", doi = "10.1590/S1415-47572007000600019" }
Đelić, N., Đelić, D., Spremo-Potparević, B., Živković, L., Marković, B., Lozanče, O.,& Blagojević, M.. (2007). Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology Soc Brasil Genetica, Ribeirao Pret., 30(4), 1144-1149. https://doi.org/10.1590/S1415-47572007000600019
Đelić N, Đelić D, Spremo-Potparević B, Živković L, Marković B, Lozanče O, Blagojević M. Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes. in Genetics and Molecular Biology. 2007;30(4):1144-1149. doi:10.1590/S1415-47572007000600019 .
Đelić, Ninoslav, Đelić, Dijana, Spremo-Potparević, Biljana, Živković, Lada, Marković, Biljana, Lozanče, Olivera, Blagojević, Miloš, "Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes" in Genetics and Molecular Biology, 30, no. 4 (2007):1144-1149, https://doi.org/10.1590/S1415-47572007000600019 . .