Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate
In vitro biosinteza ohratoksina A kod izolata Aspergillus ochraceus E'G
2009
Аутори
Bočarov-Stančić, AleksandraMiljković, Aleksandra D.
Resanović, Radmila
Nešić, Ksenija
Jaćević, Vesna
Mihaljčić, Danijela N.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This paper deals with the biosynthetic capacity for ochratoxin A (OTA) production by Aspergillus ochraceus E'G isolate derived from A. ochraceus CBS 108.08 strain, during 2007. Preliminary analysis of fungal potential for the production of OTA were performed according to the modified method of Filtenborg et al. (1983). Toxin production was tested in the following liquid media: (i) glucose-peptone-yeast extract broth (GPY - pH 5.6), (ii) potato-dextrose broth (PDB - pH 6.9), (iii) yeast extract-sucrose broth (YES - pH 6.5), and (iv) YES broth supplemented with 0.23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6.5) after stationary and submerged cultivation. Dynamics of OTA biosynthesis was tested after the cultivation of A. ochraceus E'G on natural solid substrates, such as wet sterilized rice, corn and wheat grain. Cultivations were performed during different time periods (ranging from four days to few weeks) at different temperatures (ranging from 21°C to 30°C). The presence of OTA was determined a...s follows: (i) in liquid media according to the method of Balzer et al. (1978) modified by Bočarov-Stančić et al. (2003), and (ii) in the solid substrates according to the Serbian official methods for sampling and analyzing of fodder (Official Gazette of SFRY, No. 15/87). After the cultivation of A. ochraceus E'G isolate in liquid media, the highest yield of OTA (6.4 mg/l) was obtained after submerged cultivation in PDB (4 days, 128 rpm, 21-23°C). In the case of cultivation on solid substrates, the highest amount of OTA (800.0 mg/kg of dry matter) was recorded after several week long cultivation on wheat grain at 30±1°C.
Ispitivanjem je bio obuhvaćen izolat Aspergillus ochraceus E'G izveden iz soja A. ochraceus CBS 108.08. Preliminarne analize prisustva ohratoksina A (OTA) su izvršene prema modifikovanoj metodi Filtenborg -a i sar. (1983) na agarizobanim podlogama. Proizvodnja toksina je testirana u sledećim tečnim podlogama: 1) glukoza-pepton-ekstrakt kvasca u bujonu (GPY - pH 5,6), 2) krompir - dekstroznom bujonu (PDB - pH 6,9), 3) ekstrakt kvasca - saharoznom bujonu (YES - pH 6,5) i 4) ekstrakt kvasca-saharoznom bujonu sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6,5) u uslovima stacionarne i submerzne kultivacije. Dinamika biosinteze ohratoksina A praćena je nakon gajenja na prirodnim čvrstim supstratima (zrno pirinča, kukuruza i pšenice) tokom višenedeljne kultivacije. Ohratoksin A je izolovan iz tečnih podloga za kultivaciju i produkciju primenom metode Balzera i sar. (1978) modifikovane prema Bočarov-Stančić i sar. (2003), dok je kvantitacija OTA u prirodnim čvrstim supstratima izvršena prema... Pravilniku o metodama uzimanja uzoraka i metodama fizičkih, hemijskih i mikrobioloških analiza stočne hrane ('Sl. list SFRJ', br. 15/87). Pri gajenju izolata A. ochraceus E'G u tečnim podlogama najveći prinos OTA je dobijen pri korišćenju PDB (6,4 mg/l) i to u uslovima submerzne kultivacije (4 dana, 128 o/min, 21-23°C). U slučaju kultivacije na zrnu žitarica najveću količinu OTA je izolat A. ochraceus E'G biosintetisao posle višenedeljne kultivacije na zrnu pšenice i 30±1°C (800,0 mg/kg).
Кључне речи:
Aspergillus ochraceus / ochratoxin A / in vitro biosynthesisИзвор:
Zbornik Matice srpske za prirodne nauke, 2009, 117, 69-77Издавач:
- Matica srpska, Novi Sad
Финансирање / пројекти:
- Истраживања у циљу развоја нових и побољшања постојећих формулација за контролу микотоксина у ветеринарској медицини (RS-MESTD-MPN2006-2010-23017)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Bočarov-Stančić, Aleksandra AU - Miljković, Aleksandra D. AU - Resanović, Radmila AU - Nešić, Ksenija AU - Jaćević, Vesna AU - Mihaljčić, Danijela N. PY - 2009 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/628 AB - This paper deals with the biosynthetic capacity for ochratoxin A (OTA) production by Aspergillus ochraceus E'G isolate derived from A. ochraceus CBS 108.08 strain, during 2007. Preliminary analysis of fungal potential for the production of OTA were performed according to the modified method of Filtenborg et al. (1983). Toxin production was tested in the following liquid media: (i) glucose-peptone-yeast extract broth (GPY - pH 5.6), (ii) potato-dextrose broth (PDB - pH 6.9), (iii) yeast extract-sucrose broth (YES - pH 6.5), and (iv) YES broth supplemented with 0.23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6.5) after stationary and submerged cultivation. Dynamics of OTA biosynthesis was tested after the cultivation of A. ochraceus E'G on natural solid substrates, such as wet sterilized rice, corn and wheat grain. Cultivations were performed during different time periods (ranging from four days to few weeks) at different temperatures (ranging from 21°C to 30°C). The presence of OTA was determined as follows: (i) in liquid media according to the method of Balzer et al. (1978) modified by Bočarov-Stančić et al. (2003), and (ii) in the solid substrates according to the Serbian official methods for sampling and analyzing of fodder (Official Gazette of SFRY, No. 15/87). After the cultivation of A. ochraceus E'G isolate in liquid media, the highest yield of OTA (6.4 mg/l) was obtained after submerged cultivation in PDB (4 days, 128 rpm, 21-23°C). In the case of cultivation on solid substrates, the highest amount of OTA (800.0 mg/kg of dry matter) was recorded after several week long cultivation on wheat grain at 30±1°C. AB - Ispitivanjem je bio obuhvaćen izolat Aspergillus ochraceus E'G izveden iz soja A. ochraceus CBS 108.08. Preliminarne analize prisustva ohratoksina A (OTA) su izvršene prema modifikovanoj metodi Filtenborg -a i sar. (1983) na agarizobanim podlogama. Proizvodnja toksina je testirana u sledećim tečnim podlogama: 1) glukoza-pepton-ekstrakt kvasca u bujonu (GPY - pH 5,6), 2) krompir - dekstroznom bujonu (PDB - pH 6,9), 3) ekstrakt kvasca - saharoznom bujonu (YES - pH 6,5) i 4) ekstrakt kvasca-saharoznom bujonu sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6,5) u uslovima stacionarne i submerzne kultivacije. Dinamika biosinteze ohratoksina A praćena je nakon gajenja na prirodnim čvrstim supstratima (zrno pirinča, kukuruza i pšenice) tokom višenedeljne kultivacije. Ohratoksin A je izolovan iz tečnih podloga za kultivaciju i produkciju primenom metode Balzera i sar. (1978) modifikovane prema Bočarov-Stančić i sar. (2003), dok je kvantitacija OTA u prirodnim čvrstim supstratima izvršena prema Pravilniku o metodama uzimanja uzoraka i metodama fizičkih, hemijskih i mikrobioloških analiza stočne hrane ('Sl. list SFRJ', br. 15/87). Pri gajenju izolata A. ochraceus E'G u tečnim podlogama najveći prinos OTA je dobijen pri korišćenju PDB (6,4 mg/l) i to u uslovima submerzne kultivacije (4 dana, 128 o/min, 21-23°C). U slučaju kultivacije na zrnu žitarica najveću količinu OTA je izolat A. ochraceus E'G biosintetisao posle višenedeljne kultivacije na zrnu pšenice i 30±1°C (800,0 mg/kg). PB - Matica srpska, Novi Sad T2 - Zbornik Matice srpske za prirodne nauke T1 - Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate T1 - In vitro biosinteza ohratoksina A kod izolata Aspergillus ochraceus E'G IS - 117 SP - 69 EP - 77 DO - 10.2298/ZMSPN0917069B ER -
@article{ author = "Bočarov-Stančić, Aleksandra and Miljković, Aleksandra D. and Resanović, Radmila and Nešić, Ksenija and Jaćević, Vesna and Mihaljčić, Danijela N.", year = "2009", abstract = "This paper deals with the biosynthetic capacity for ochratoxin A (OTA) production by Aspergillus ochraceus E'G isolate derived from A. ochraceus CBS 108.08 strain, during 2007. Preliminary analysis of fungal potential for the production of OTA were performed according to the modified method of Filtenborg et al. (1983). Toxin production was tested in the following liquid media: (i) glucose-peptone-yeast extract broth (GPY - pH 5.6), (ii) potato-dextrose broth (PDB - pH 6.9), (iii) yeast extract-sucrose broth (YES - pH 6.5), and (iv) YES broth supplemented with 0.23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6.5) after stationary and submerged cultivation. Dynamics of OTA biosynthesis was tested after the cultivation of A. ochraceus E'G on natural solid substrates, such as wet sterilized rice, corn and wheat grain. Cultivations were performed during different time periods (ranging from four days to few weeks) at different temperatures (ranging from 21°C to 30°C). The presence of OTA was determined as follows: (i) in liquid media according to the method of Balzer et al. (1978) modified by Bočarov-Stančić et al. (2003), and (ii) in the solid substrates according to the Serbian official methods for sampling and analyzing of fodder (Official Gazette of SFRY, No. 15/87). After the cultivation of A. ochraceus E'G isolate in liquid media, the highest yield of OTA (6.4 mg/l) was obtained after submerged cultivation in PDB (4 days, 128 rpm, 21-23°C). In the case of cultivation on solid substrates, the highest amount of OTA (800.0 mg/kg of dry matter) was recorded after several week long cultivation on wheat grain at 30±1°C., Ispitivanjem je bio obuhvaćen izolat Aspergillus ochraceus E'G izveden iz soja A. ochraceus CBS 108.08. Preliminarne analize prisustva ohratoksina A (OTA) su izvršene prema modifikovanoj metodi Filtenborg -a i sar. (1983) na agarizobanim podlogama. Proizvodnja toksina je testirana u sledećim tečnim podlogama: 1) glukoza-pepton-ekstrakt kvasca u bujonu (GPY - pH 5,6), 2) krompir - dekstroznom bujonu (PDB - pH 6,9), 3) ekstrakt kvasca - saharoznom bujonu (YES - pH 6,5) i 4) ekstrakt kvasca-saharoznom bujonu sa dodatkom 0,23 mg/l ZnSO4 x 5 H2O (YESZn - pH 6,5) u uslovima stacionarne i submerzne kultivacije. Dinamika biosinteze ohratoksina A praćena je nakon gajenja na prirodnim čvrstim supstratima (zrno pirinča, kukuruza i pšenice) tokom višenedeljne kultivacije. Ohratoksin A je izolovan iz tečnih podloga za kultivaciju i produkciju primenom metode Balzera i sar. (1978) modifikovane prema Bočarov-Stančić i sar. (2003), dok je kvantitacija OTA u prirodnim čvrstim supstratima izvršena prema Pravilniku o metodama uzimanja uzoraka i metodama fizičkih, hemijskih i mikrobioloških analiza stočne hrane ('Sl. list SFRJ', br. 15/87). Pri gajenju izolata A. ochraceus E'G u tečnim podlogama najveći prinos OTA je dobijen pri korišćenju PDB (6,4 mg/l) i to u uslovima submerzne kultivacije (4 dana, 128 o/min, 21-23°C). U slučaju kultivacije na zrnu žitarica najveću količinu OTA je izolat A. ochraceus E'G biosintetisao posle višenedeljne kultivacije na zrnu pšenice i 30±1°C (800,0 mg/kg).", publisher = "Matica srpska, Novi Sad", journal = "Zbornik Matice srpske za prirodne nauke", title = "Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate, In vitro biosinteza ohratoksina A kod izolata Aspergillus ochraceus E'G", number = "117", pages = "69-77", doi = "10.2298/ZMSPN0917069B" }
Bočarov-Stančić, A., Miljković, A. D., Resanović, R., Nešić, K., Jaćević, V.,& Mihaljčić, D. N.. (2009). Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate. in Zbornik Matice srpske za prirodne nauke Matica srpska, Novi Sad.(117), 69-77. https://doi.org/10.2298/ZMSPN0917069B
Bočarov-Stančić A, Miljković AD, Resanović R, Nešić K, Jaćević V, Mihaljčić DN. Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate. in Zbornik Matice srpske za prirodne nauke. 2009;(117):69-77. doi:10.2298/ZMSPN0917069B .
Bočarov-Stančić, Aleksandra, Miljković, Aleksandra D., Resanović, Radmila, Nešić, Ksenija, Jaćević, Vesna, Mihaljčić, Danijela N., "Ochratoxin A 'in vitro' biosynthesis by the Aspergillus ochraceus E'G isolate" in Zbornik Matice srpske za prirodne nauke, no. 117 (2009):69-77, https://doi.org/10.2298/ZMSPN0917069B . .