Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout
Нема приказа
Аутори
Đorđević, V.Baltić, Milan Ž.
Matekalo-Sverak, Vesna
Karabasil, Nedjeljko
Ćirković, Miroslav
Milijašević, Milan
Dimitrijević, Mirjana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aeromonas hydrophila spp. is commonly present in water and in certain incidences they might cause diseases in fish and amphibians. Humans are infected by ingestion of these bacteria with contaminated food or water. Aeromonas hydrophila spp. might cause gastro-intestinal disorders and poisoning in humans. One of the major issues associated with Aeromonas hydrophila and incidence of gastro-intestinal diseases is accurate identification of aeromonades at the species level. Therefore, there is a need for a clear phenotypic scheme and biochemical characterization of the mentioned species. Objective of this paper was to study the presence of Aeromas hydrophila in trout samples and to test biochemical characteristics of isolated strains. For isolation of Aeromonas hydrophila species, 120 samples of rainbow trout, commercially available, were studied. Aeromonas hydrophila was isolated from fish muscle tissue and skin according to "Microbiological Methods for the Meat Industry", second edition ...(1991) Biochemical studies were performed on Aeromonas hydrophila medium, followed by oxidase test (+), catalase test (+), staining according to Gram (-) and vibriostat test agar (0/129, vibriostatic), as well as biochemical tests API 20E and API 20NE. From the 120 studied rainbow trout samples, 12 strains of Aeromonas hydrophila were isolated, out of which seven strains (58.3%) of Aeromonas hydrophila belonged to group 1, whereas five strains (41,6%) belonged to Aeromonas hydrophila group 2. Out of 12 isolated strains, 11 (91.7%) had β-galactosidase. They were citrate and indol positive, sorbitol negative, arabinose positive, NO2 - producing and reducing it to N2. Then (83.3%) of 12 twelve isolated strains were Voges-Proskauer positive and melibiose negative, whereas eight (66.7%) were amigdalin and lysine-decarboxylase positive. It was established that seven of 12 twelve strains (58.3%) ferment glucose. The obtained results demonstrate the need to supplement phenotypic schemes for identification of Aeromonas hydrophila strains originating from fish at the species level.
Кључне речи:
Aeromonas hydrophila / Biochemical characterization / Rainbow teout (Oncorhynchus Mykiss)Извор:
CEFood 2012 - Proceedings of 6th Central European Congress on Food, 2012, 364-369Издавач:
- University of Novi Sad, Faculty of Technology
Scopus: 2-s2.0-84926069437
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Đorđević, V. AU - Baltić, Milan Ž. AU - Matekalo-Sverak, Vesna AU - Karabasil, Nedjeljko AU - Ćirković, Miroslav AU - Milijašević, Milan AU - Dimitrijević, Mirjana PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/898 AB - Aeromonas hydrophila spp. is commonly present in water and in certain incidences they might cause diseases in fish and amphibians. Humans are infected by ingestion of these bacteria with contaminated food or water. Aeromonas hydrophila spp. might cause gastro-intestinal disorders and poisoning in humans. One of the major issues associated with Aeromonas hydrophila and incidence of gastro-intestinal diseases is accurate identification of aeromonades at the species level. Therefore, there is a need for a clear phenotypic scheme and biochemical characterization of the mentioned species. Objective of this paper was to study the presence of Aeromas hydrophila in trout samples and to test biochemical characteristics of isolated strains. For isolation of Aeromonas hydrophila species, 120 samples of rainbow trout, commercially available, were studied. Aeromonas hydrophila was isolated from fish muscle tissue and skin according to "Microbiological Methods for the Meat Industry", second edition (1991) Biochemical studies were performed on Aeromonas hydrophila medium, followed by oxidase test (+), catalase test (+), staining according to Gram (-) and vibriostat test agar (0/129, vibriostatic), as well as biochemical tests API 20E and API 20NE. From the 120 studied rainbow trout samples, 12 strains of Aeromonas hydrophila were isolated, out of which seven strains (58.3%) of Aeromonas hydrophila belonged to group 1, whereas five strains (41,6%) belonged to Aeromonas hydrophila group 2. Out of 12 isolated strains, 11 (91.7%) had β-galactosidase. They were citrate and indol positive, sorbitol negative, arabinose positive, NO2 - producing and reducing it to N2. Then (83.3%) of 12 twelve isolated strains were Voges-Proskauer positive and melibiose negative, whereas eight (66.7%) were amigdalin and lysine-decarboxylase positive. It was established that seven of 12 twelve strains (58.3%) ferment glucose. The obtained results demonstrate the need to supplement phenotypic schemes for identification of Aeromonas hydrophila strains originating from fish at the species level. PB - University of Novi Sad, Faculty of Technology C3 - CEFood 2012 - Proceedings of 6th Central European Congress on Food T1 - Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout SP - 364 EP - 369 UR - https://hdl.handle.net/21.15107/rcub_veterinar_898 ER -
@conference{ author = "Đorđević, V. and Baltić, Milan Ž. and Matekalo-Sverak, Vesna and Karabasil, Nedjeljko and Ćirković, Miroslav and Milijašević, Milan and Dimitrijević, Mirjana", year = "2012", abstract = "Aeromonas hydrophila spp. is commonly present in water and in certain incidences they might cause diseases in fish and amphibians. Humans are infected by ingestion of these bacteria with contaminated food or water. Aeromonas hydrophila spp. might cause gastro-intestinal disorders and poisoning in humans. One of the major issues associated with Aeromonas hydrophila and incidence of gastro-intestinal diseases is accurate identification of aeromonades at the species level. Therefore, there is a need for a clear phenotypic scheme and biochemical characterization of the mentioned species. Objective of this paper was to study the presence of Aeromas hydrophila in trout samples and to test biochemical characteristics of isolated strains. For isolation of Aeromonas hydrophila species, 120 samples of rainbow trout, commercially available, were studied. Aeromonas hydrophila was isolated from fish muscle tissue and skin according to "Microbiological Methods for the Meat Industry", second edition (1991) Biochemical studies were performed on Aeromonas hydrophila medium, followed by oxidase test (+), catalase test (+), staining according to Gram (-) and vibriostat test agar (0/129, vibriostatic), as well as biochemical tests API 20E and API 20NE. From the 120 studied rainbow trout samples, 12 strains of Aeromonas hydrophila were isolated, out of which seven strains (58.3%) of Aeromonas hydrophila belonged to group 1, whereas five strains (41,6%) belonged to Aeromonas hydrophila group 2. Out of 12 isolated strains, 11 (91.7%) had β-galactosidase. They were citrate and indol positive, sorbitol negative, arabinose positive, NO2 - producing and reducing it to N2. Then (83.3%) of 12 twelve isolated strains were Voges-Proskauer positive and melibiose negative, whereas eight (66.7%) were amigdalin and lysine-decarboxylase positive. It was established that seven of 12 twelve strains (58.3%) ferment glucose. The obtained results demonstrate the need to supplement phenotypic schemes for identification of Aeromonas hydrophila strains originating from fish at the species level.", publisher = "University of Novi Sad, Faculty of Technology", journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food", title = "Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout", pages = "364-369", url = "https://hdl.handle.net/21.15107/rcub_veterinar_898" }
Đorđević, V., Baltić, M. Ž., Matekalo-Sverak, V., Karabasil, N., Ćirković, M., Milijašević, M.,& Dimitrijević, M.. (2012). Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout. in CEFood 2012 - Proceedings of 6th Central European Congress on Food University of Novi Sad, Faculty of Technology., 364-369. https://hdl.handle.net/21.15107/rcub_veterinar_898
Đorđević V, Baltić MŽ, Matekalo-Sverak V, Karabasil N, Ćirković M, Milijašević M, Dimitrijević M. Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:364-369. https://hdl.handle.net/21.15107/rcub_veterinar_898 .
Đorđević, V., Baltić, Milan Ž., Matekalo-Sverak, Vesna, Karabasil, Nedjeljko, Ćirković, Miroslav, Milijašević, Milan, Dimitrijević, Mirjana, "Isolation and biochemical properties of Aeromonas hydrophila hydrophila in rainbow trout" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):364-369, https://hdl.handle.net/21.15107/rcub_veterinar_898 .