Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects
2012
Аутори
Tasić, AleksandarTasic-Otasević, Suzana
Gabrielli, Simona
Miladinovic-Tasić, Nataša
Ignjatović, Aleksandra
Đorđević, Jovana
Dimitrijević, Sanda
Cancrini, Gabriella
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In 2009 canine filarial infections were investigated in two northern areas of Serbia (Pancevo and Veliko Gradiste), applying morphometry, biochemical staining, and immunological kit to detect Dirofilaria immitis antigens, and two home-made ELISAs to detect antibodies to D. repens and D. immitis somatic/metabolic polyproteins. Moreover, molecular tools were applied to analyze the phylogenetic relationships of the isolates. The microfilariae detected in 21/122 dogs (17.2%) were identified as D. repens (n = 21) and D. immitis (n = 2). D. immitis antigens were found in another 13 animals with occult infection. All of the 15 heartworm-positive dogs also had antibodies to this parasite, which were detected in another 13 subjects, indicating an overall D. immitis seroprevalence rate of 22.9%. Serology for D. repens revealed evidence of antibodies in 42.6% of the dogs, but was negative for 4 microfilaremic dogs. As for the two different areas, the prevalence of microfilariae and/or D. immitis ...antigens, mainly due to D. repens microfilaremic animals, was not significantly higher in Veliko Gradiste (33.3%) than in Pancevo (22%). However, serology showed a different epidemiological picture. Heartworm infection occurred more often in both areas, and antibodies to dirofilarial nematodes were detected in 72.9% of dogs living in Pancevo, a rate higher than in those living in Veliko Gradiste (57.1%). No risk factors for infection were found, confirming the uselessness of prophylactic drugs against D. repens, and suggesting the presence in these areas of sunrise- or sunset-biting mosquitoes as important vectors. The results indicate the need for both appropriate entomological studies and further research on the intra-species variability shown by D. repens.
Кључне речи:
Dirofilaria / Epidemiology / Genetics / SerbiaИзвор:
Vector-Borne and Zoonotic Diseases, 2012, 12, 12, 1031-1035Издавач:
- Mary Ann Liebert Inc, New Rochelle
Финансирање / пројекти:
- Значај доказивања раних лабораторијских биомаркера за исход инвазивних гљивичних инфекција код нас (RS-MESTD-Basic Research (BR or ON)-175034)
DOI: 10.1089/vbz.2011.0949
ISSN: 1530-3667
PubMed: 23127188
WoS: 000312492800004
Scopus: 2-s2.0-84871221060
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Tasić, Aleksandar AU - Tasic-Otasević, Suzana AU - Gabrielli, Simona AU - Miladinovic-Tasić, Nataša AU - Ignjatović, Aleksandra AU - Đorđević, Jovana AU - Dimitrijević, Sanda AU - Cancrini, Gabriella PY - 2012 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/902 AB - In 2009 canine filarial infections were investigated in two northern areas of Serbia (Pancevo and Veliko Gradiste), applying morphometry, biochemical staining, and immunological kit to detect Dirofilaria immitis antigens, and two home-made ELISAs to detect antibodies to D. repens and D. immitis somatic/metabolic polyproteins. Moreover, molecular tools were applied to analyze the phylogenetic relationships of the isolates. The microfilariae detected in 21/122 dogs (17.2%) were identified as D. repens (n = 21) and D. immitis (n = 2). D. immitis antigens were found in another 13 animals with occult infection. All of the 15 heartworm-positive dogs also had antibodies to this parasite, which were detected in another 13 subjects, indicating an overall D. immitis seroprevalence rate of 22.9%. Serology for D. repens revealed evidence of antibodies in 42.6% of the dogs, but was negative for 4 microfilaremic dogs. As for the two different areas, the prevalence of microfilariae and/or D. immitis antigens, mainly due to D. repens microfilaremic animals, was not significantly higher in Veliko Gradiste (33.3%) than in Pancevo (22%). However, serology showed a different epidemiological picture. Heartworm infection occurred more often in both areas, and antibodies to dirofilarial nematodes were detected in 72.9% of dogs living in Pancevo, a rate higher than in those living in Veliko Gradiste (57.1%). No risk factors for infection were found, confirming the uselessness of prophylactic drugs against D. repens, and suggesting the presence in these areas of sunrise- or sunset-biting mosquitoes as important vectors. The results indicate the need for both appropriate entomological studies and further research on the intra-species variability shown by D. repens. PB - Mary Ann Liebert Inc, New Rochelle T2 - Vector-Borne and Zoonotic Diseases T1 - Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects VL - 12 IS - 12 SP - 1031 EP - 1035 DO - 10.1089/vbz.2011.0949 ER -
@article{ author = "Tasić, Aleksandar and Tasic-Otasević, Suzana and Gabrielli, Simona and Miladinovic-Tasić, Nataša and Ignjatović, Aleksandra and Đorđević, Jovana and Dimitrijević, Sanda and Cancrini, Gabriella", year = "2012", abstract = "In 2009 canine filarial infections were investigated in two northern areas of Serbia (Pancevo and Veliko Gradiste), applying morphometry, biochemical staining, and immunological kit to detect Dirofilaria immitis antigens, and two home-made ELISAs to detect antibodies to D. repens and D. immitis somatic/metabolic polyproteins. Moreover, molecular tools were applied to analyze the phylogenetic relationships of the isolates. The microfilariae detected in 21/122 dogs (17.2%) were identified as D. repens (n = 21) and D. immitis (n = 2). D. immitis antigens were found in another 13 animals with occult infection. All of the 15 heartworm-positive dogs also had antibodies to this parasite, which were detected in another 13 subjects, indicating an overall D. immitis seroprevalence rate of 22.9%. Serology for D. repens revealed evidence of antibodies in 42.6% of the dogs, but was negative for 4 microfilaremic dogs. As for the two different areas, the prevalence of microfilariae and/or D. immitis antigens, mainly due to D. repens microfilaremic animals, was not significantly higher in Veliko Gradiste (33.3%) than in Pancevo (22%). However, serology showed a different epidemiological picture. Heartworm infection occurred more often in both areas, and antibodies to dirofilarial nematodes were detected in 72.9% of dogs living in Pancevo, a rate higher than in those living in Veliko Gradiste (57.1%). No risk factors for infection were found, confirming the uselessness of prophylactic drugs against D. repens, and suggesting the presence in these areas of sunrise- or sunset-biting mosquitoes as important vectors. The results indicate the need for both appropriate entomological studies and further research on the intra-species variability shown by D. repens.", publisher = "Mary Ann Liebert Inc, New Rochelle", journal = "Vector-Borne and Zoonotic Diseases", title = "Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects", volume = "12", number = "12", pages = "1031-1035", doi = "10.1089/vbz.2011.0949" }
Tasić, A., Tasic-Otasević, S., Gabrielli, S., Miladinovic-Tasić, N., Ignjatović, A., Đorđević, J., Dimitrijević, S.,& Cancrini, G.. (2012). Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects. in Vector-Borne and Zoonotic Diseases Mary Ann Liebert Inc, New Rochelle., 12(12), 1031-1035. https://doi.org/10.1089/vbz.2011.0949
Tasić A, Tasic-Otasević S, Gabrielli S, Miladinovic-Tasić N, Ignjatović A, Đorđević J, Dimitrijević S, Cancrini G. Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects. in Vector-Borne and Zoonotic Diseases. 2012;12(12):1031-1035. doi:10.1089/vbz.2011.0949 .
Tasić, Aleksandar, Tasic-Otasević, Suzana, Gabrielli, Simona, Miladinovic-Tasić, Nataša, Ignjatović, Aleksandra, Đorđević, Jovana, Dimitrijević, Sanda, Cancrini, Gabriella, "Canine Dirofilaria Infections in Two Uninvestigated Areas of Serbia: Epidemiological and Genetic Aspects" in Vector-Borne and Zoonotic Diseases, 12, no. 12 (2012):1031-1035, https://doi.org/10.1089/vbz.2011.0949 . .