TY  - JOUR
AU  - Epps, Sharon V. R.
AU  - Petrujkić, Branko
AU  - Sedej, Ivana
AU  - Krueger, Nathan A.
AU  - Harvey, Roger B.
AU  - Beier, Ross C.
AU  - Stanton, Thad B.
AU  - Phillips, Timothy D.
AU  - Anderson, Robin C.
AU  - Nisbet, David J.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1218
AB  - Thymol is a natural product that exhibits antimicrobial activity in vitro but in vivo results indicate that absorption within the proximal alimentary tract precludes its delivery to the distal gut. Presently, the anti-Campylobacter activity of thymol was compared against that of thymol-beta-D-glucopyranoside, the latter being resistant to absorption. When treated with 1 mM thymol, Campylobacter coli and jejuni were reduced during pure or co-culture with a beta-glycoside-hydrolysing Parabacteroides distasonis. Thymol-beta-D-glucopyranoside treatment (1 mM) did not reduce C coli and jejuni during pure culture but did during co-culture with P. distasonis or during mixed culture with porcine or bovine faecal microbes possessing beta-glycoside-hydrolysing activity. Fermentation acid production was reduced by thymol-beta-D-glucopyranoside treatment, indicating that fermentation was inhibited, which may limit its application to just before harvest. Results suggest that thymol-beta-D-glucopyranoside or similar beta-glycosides may be able to escape absorption within the proximal gut and become activated by bacterial beta-glycosidases in the distal gut. Published by Elsevier Ltd.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Comparison of anti-Campylobacter activity of free thymol and thymol-beta-D-glucopyranoside in absence or presence of beta-glycoside-hydrolysing gut bacteria
VL  - 173
SP  - 92
EP  - 98
DO  - 10.1016/j.foodchem.2014.10.007
ER  - 

@article{
author = "Epps, Sharon V. R. and Petrujkić, Branko and Sedej, Ivana and Krueger, Nathan A. and Harvey, Roger B. and Beier, Ross C. and Stanton, Thad B. and Phillips, Timothy D. and Anderson, Robin C. and Nisbet, David J.",
year = "2015",
abstract = "Thymol is a natural product that exhibits antimicrobial activity in vitro but in vivo results indicate that absorption within the proximal alimentary tract precludes its delivery to the distal gut. Presently, the anti-Campylobacter activity of thymol was compared against that of thymol-beta-D-glucopyranoside, the latter being resistant to absorption. When treated with 1 mM thymol, Campylobacter coli and jejuni were reduced during pure or co-culture with a beta-glycoside-hydrolysing Parabacteroides distasonis. Thymol-beta-D-glucopyranoside treatment (1 mM) did not reduce C coli and jejuni during pure culture but did during co-culture with P. distasonis or during mixed culture with porcine or bovine faecal microbes possessing beta-glycoside-hydrolysing activity. Fermentation acid production was reduced by thymol-beta-D-glucopyranoside treatment, indicating that fermentation was inhibited, which may limit its application to just before harvest. Results suggest that thymol-beta-D-glucopyranoside or similar beta-glycosides may be able to escape absorption within the proximal gut and become activated by bacterial beta-glycosidases in the distal gut. Published by Elsevier Ltd.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Comparison of anti-Campylobacter activity of free thymol and thymol-beta-D-glucopyranoside in absence or presence of beta-glycoside-hydrolysing gut bacteria",
volume = "173",
pages = "92-98",
doi = "10.1016/j.foodchem.2014.10.007"
}

Epps, S. V. R., Petrujkić, B., Sedej, I., Krueger, N. A., Harvey, R. B., Beier, R. C., Stanton, T. B., Phillips, T. D., Anderson, R. C.,& Nisbet, D. J.. (2015). Comparison of anti-Campylobacter activity of free thymol and thymol-beta-D-glucopyranoside in absence or presence of beta-glycoside-hydrolysing gut bacteria. in Food Chemistry
Elsevier Sci Ltd, Oxford., 173, 92-98.
https://doi.org/10.1016/j.foodchem.2014.10.007

Epps SVR, Petrujkić B, Sedej I, Krueger NA, Harvey RB, Beier RC, Stanton TB, Phillips TD, Anderson RC, Nisbet DJ. Comparison of anti-Campylobacter activity of free thymol and thymol-beta-D-glucopyranoside in absence or presence of beta-glycoside-hydrolysing gut bacteria. in Food Chemistry. 2015;173:92-98.
doi:10.1016/j.foodchem.2014.10.007 .

Epps, Sharon V. R., Petrujkić, Branko, Sedej, Ivana, Krueger, Nathan A., Harvey, Roger B., Beier, Ross C., Stanton, Thad B., Phillips, Timothy D., Anderson, Robin C., Nisbet, David J., "Comparison of anti-Campylobacter activity of free thymol and thymol-beta-D-glucopyranoside in absence or presence of beta-glycoside-hydrolysing gut bacteria" in Food Chemistry, 173 (2015):92-98,
https://doi.org/10.1016/j.foodchem.2014.10.007 . .

TY  - JOUR
AU  - Petrujkić, Branko
AU  - Sedej, Ivana
AU  - Beier, Ross C.
AU  - Anderson, Robin C.
AU  - Harvey, Roger B.
AU  - Epps, Sharon V. R.
AU  - Stipanović, Robert D.
AU  - Krueger, Nathan A.
AU  - Nisbet, David J.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1016
AB  - Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-beta-D-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0-500 mg/L thymol. From 1 and 3 mM solutions, 0.293 +/- 0.04 and 0.898 +/- 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 +/- 0.041 and 0.317 +/- 0.143 mM thymol-beta-D-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-beta-D-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence that thymol-beta-D-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments
VL  - 61
IS  - 15
SP  - 3757
EP  - 3762
DO  - 10.1021/jf401013a
ER  - 

@article{
author = "Petrujkić, Branko and Sedej, Ivana and Beier, Ross C. and Anderson, Robin C. and Harvey, Roger B. and Epps, Sharon V. R. and Stipanović, Robert D. and Krueger, Nathan A. and Nisbet, David J.",
year = "2013",
abstract = "Food-producing animals are reservoirs of Campylobacter, a leading bacterial cause of human foodborne illness. The natural product thymol can reduce the survivability of Campylobacter, but its rapid absorption in the proximal gastrointestinal tract may preclude its use as a feed additive to reduce intestinal colonization of these pathogens. This work examined the ex vivo absorption of thymol and thymol-beta-D-glucopyranoside in everted porcine jejunal segments, as the latter was hypothesized to be more resistant to absorption. A modified gas chromatography and extraction method was developed to determine 1.0-500 mg/L thymol. From 1 and 3 mM solutions, 0.293 +/- 0.04 and 0.898 +/- 0.212 mM thymol, respectively, p = 0.0347, were absorbed, and 0.125 +/- 0.041 and 0.317 +/- 0.143 mM thymol-beta-D-glucopyranoside, respectively, p = 0.0892, were absorbed. Results indicate that thymol-beta-D-glucopyranoside was absorbed 2.3 to 2.8 times less effectively than thymol, thus providing evidence that thymol-beta-D-glucopyranoside may potentially be used as a feed additive to transport thymol to the piglet lower gut.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments",
volume = "61",
number = "15",
pages = "3757-3762",
doi = "10.1021/jf401013a"
}

Petrujkić, B., Sedej, I., Beier, R. C., Anderson, R. C., Harvey, R. B., Epps, S. V. R., Stipanović, R. D., Krueger, N. A.,& Nisbet, D. J.. (2013). Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 61(15), 3757-3762.
https://doi.org/10.1021/jf401013a

Petrujkić B, Sedej I, Beier RC, Anderson RC, Harvey RB, Epps SVR, Stipanović RD, Krueger NA, Nisbet DJ. Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments. in Journal of Agricultural and Food Chemistry. 2013;61(15):3757-3762.
doi:10.1021/jf401013a .

Petrujkić, Branko, Sedej, Ivana, Beier, Ross C., Anderson, Robin C., Harvey, Roger B., Epps, Sharon V. R., Stipanović, Robert D., Krueger, Nathan A., Nisbet, David J., "Ex Vivo Absorption of Thymol and Thymol-beta-D-glucopyranoside in Piglet Everted Jejunal Segments" in Journal of Agricultural and Food Chemistry, 61, no. 15 (2013):3757-3762,
https://doi.org/10.1021/jf401013a . .