@conference{
author = "Maletić, Milan and Đukić, Božidar and Nedić, Svetlana and Stanišić, Ljubodrag and Stanimirović, Zoran and Stevanović, Jevrosima and Vakanjac, Slobodanka",
year = "2016",
abstract = "Mastitis is the most common disease in dairy cattle and presents the most costly disorder
to the dairy industry. Subclinical mastitis is inflammation of the mammary gland that does
not create visible changes in the milk or the udder, thus, subclinically infected cows will
produce less milk, and the quality of the milk will be reduced. In addition, infected cows
can be a source of infection to other animals in the herd. Among other unspecified causative
agents of mastitis, algae Prototheca spp. is not included in routine diagnosis in laboratories.
However, Prototheca spp infections usually result in a chronic subclinical or mild clinical,
inflammatory process in the udder, and were followed by a dramatic loss in milk production
and a permanent increase in somatic cell count. This type of infection is rare, but the
incidence of reported cases is increasing worldwide. In our investigation, 605 milk samples
were obtained fromdairy cows in a total of 5 herds with a history of increasing somatic cell
counts, subclinical and mild clinical signs of udder infection, and/or unsuccessful response
to the usual therapy. Microbiological isolation of algae on Blood agar and Sabouraud agar
at 25°C and 37°C, respectively, showed that 39 (6.45%) samples were positive on
Prototheca spp. which was confirmed microscopically. The SCC of Prototheca spp.
positive milk samples were from 5×105
/mL up to 13 ×106
/mL. Considering the wide
distribution of these algae as saprophytes in the environment, further investigation should
include detail microbiological and molecular techniques in order to reveal genotypes
involved prevent infections and decrease economic losses.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "International Symposium on Animal Science (ISAS), Belgrade, 24 - 25 November, 2016",
title = "Bovine subclinical mastitis associated with Prototheca spp.",
pages = "327-333",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3825"
}