TY  - JOUR
AU  - Ćupić Miladinović, Dejana
AU  - Prevendar Crnić, Andreja
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Ivanović, Saša
AU  - Santibanez, Juan F.
AU  - Ćupić, Vitomir
AU  - Borozan, Nevena
AU  - Antonijević Miljaković, Evica
AU  - Borozan, Sunčica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2018
AB  - Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration
VL  - 333
DO  - 10.1016/j.cbi.2020.109312
ER  - 

@article{
author = "Ćupić Miladinović, Dejana and Prevendar Crnić, Andreja and Peković, Sanja and Dacić, Sanja and Ivanović, Saša and Santibanez, Juan F. and Ćupić, Vitomir and Borozan, Nevena and Antonijević Miljaković, Evica and Borozan, Sunčica",
year = "2021",
abstract = "Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration",
volume = "333",
doi = "10.1016/j.cbi.2020.109312"
}

Ćupić Miladinović, D., Prevendar Crnić, A., Peković, S., Dacić, S., Ivanović, S., Santibanez, J. F., Ćupić, V., Borozan, N., Antonijević Miljaković, E.,& Borozan, S.. (2021). Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions
Elsevier., 333.
https://doi.org/10.1016/j.cbi.2020.109312

Ćupić Miladinović D, Prevendar Crnić A, Peković S, Dacić S, Ivanović S, Santibanez JF, Ćupić V, Borozan N, Antonijević Miljaković E, Borozan S. Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions. 2021;333.
doi:10.1016/j.cbi.2020.109312 .

Ćupić Miladinović, Dejana, Prevendar Crnić, Andreja, Peković, Sanja, Dacić, Sanja, Ivanović, Saša, Santibanez, Juan F., Ćupić, Vitomir, Borozan, Nevena, Antonijević Miljaković, Evica, Borozan, Sunčica, "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration" in Chemico-Biological Interactions, 333 (2021),
https://doi.org/10.1016/j.cbi.2020.109312 . .

TY  - CONF
AU  - Ćupić Miladinović, Dejana
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Filipović, Isidora
AU  - Životić, Maja
AU  - Đorđević, Milena
AU  - Blagojević, Miloš
AU  - Nešić, Ivana
AU  - Borozan, Sunčica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2640
AB  - The aim of this study was to investigate the influence of vitamin B1 (thiamine) following thepathohystologycalchanges in the hippocampus, cerebellum and cerebral cortex of Japanese quail (Coturnix japanica) treated with chlorpyrifos.In this study we also assessed the antioxidative activity of thiamine in the brain by monitoring the nitrite concentration(NO2-), parameter of oxidative/nitrosative stress and activities of agents of cellular detoxification such as glutathione(GSH) and glutathione S-transferase(GST).
The study was conducted onforty male Japanese quails (2 controls and 2 experimental groups, n= 10), 3-4 weeks old. One controlgroup was treated only with vitamin B1, while the second one receivedpurecorn oil.CPFdissolved in corn oil was administeredto quails by gavage for 7 consecutive days at dose of 3 mg/kg BW while another groups wastreated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration for 7 consecutive days.
Our studies have shown that CPF has ledto increase in the concentration of NO2-,but after thiamine treatment there has been a decrease. Also CPF has led to small changes in GSH and GST levels, while groups treated with vitamin B1showedsignificantly (p< 0.0001)increased activity of these parameteres, proving very important role of thiamine in the detoxification and elimination of  pesticides.
In hippocampus groups that received CPF showed signs of edema with numerous damaged neurons, especially in pyramidal layer, while in groups that received vitamin B1 along with CPF, pathological changes were similar, but less prominent.In cerebellum groups that received CPF showed large number of degenerated Purkinje cells, while with vitamin B1 the reduction of degenerated neurons is present.Cerebral cortex showed degeneration with pycnotic nuclei of many neurons, edema and congestion in groups which received CPF and also similar changes were found after application of B1.
Overall these results confirm that CPF causes oxidative stress and degenerative changes, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".
PB  - Beograd: Društvo za neuronauke Srbije
C3  - FENS - Regional meeting, Belgrade, July 10-13 2019
T1  - What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos
SP  - 486
EP  - 486
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2640
ER  - 

@conference{
author = "Ćupić Miladinović, Dejana and Peković, Sanja and Dacić, Sanja and Filipović, Isidora and Životić, Maja and Đorđević, Milena and Blagojević, Miloš and Nešić, Ivana and Borozan, Sunčica",
year = "2019",
abstract = "The aim of this study was to investigate the influence of vitamin B1 (thiamine) following thepathohystologycalchanges in the hippocampus, cerebellum and cerebral cortex of Japanese quail (Coturnix japanica) treated with chlorpyrifos.In this study we also assessed the antioxidative activity of thiamine in the brain by monitoring the nitrite concentration(NO2-), parameter of oxidative/nitrosative stress and activities of agents of cellular detoxification such as glutathione(GSH) and glutathione S-transferase(GST).
The study was conducted onforty male Japanese quails (2 controls and 2 experimental groups, n= 10), 3-4 weeks old. One controlgroup was treated only with vitamin B1, while the second one receivedpurecorn oil.CPFdissolved in corn oil was administeredto quails by gavage for 7 consecutive days at dose of 3 mg/kg BW while another groups wastreated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration for 7 consecutive days.
Our studies have shown that CPF has ledto increase in the concentration of NO2-,but after thiamine treatment there has been a decrease. Also CPF has led to small changes in GSH and GST levels, while groups treated with vitamin B1showedsignificantly (p< 0.0001)increased activity of these parameteres, proving very important role of thiamine in the detoxification and elimination of  pesticides.
In hippocampus groups that received CPF showed signs of edema with numerous damaged neurons, especially in pyramidal layer, while in groups that received vitamin B1 along with CPF, pathological changes were similar, but less prominent.In cerebellum groups that received CPF showed large number of degenerated Purkinje cells, while with vitamin B1 the reduction of degenerated neurons is present.Cerebral cortex showed degeneration with pycnotic nuclei of many neurons, edema and congestion in groups which received CPF and also similar changes were found after application of B1.
Overall these results confirm that CPF causes oxidative stress and degenerative changes, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".",
publisher = "Beograd: Društvo za neuronauke Srbije",
journal = "FENS - Regional meeting, Belgrade, July 10-13 2019",
title = "What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos",
pages = "486-486",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2640"
}

Ćupić Miladinović, D., Peković, S., Dacić, S., Filipović, I., Životić, M., Đorđević, M., Blagojević, M., Nešić, I.,& Borozan, S.. (2019). What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos. in FENS - Regional meeting, Belgrade, July 10-13 2019
Beograd: Društvo za neuronauke Srbije., 486-486.
https://hdl.handle.net/21.15107/rcub_veterinar_2640

Ćupić Miladinović D, Peković S, Dacić S, Filipović I, Životić M, Đorđević M, Blagojević M, Nešić I, Borozan S. What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos. in FENS - Regional meeting, Belgrade, July 10-13 2019. 2019;:486-486.
https://hdl.handle.net/21.15107/rcub_veterinar_2640 .

Ćupić Miladinović, Dejana, Peković, Sanja, Dacić, Sanja, Filipović, Isidora, Životić, Maja, Đorđević, Milena, Blagojević, Miloš, Nešić, Ivana, Borozan, Sunčica, "What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos" in FENS - Regional meeting, Belgrade, July 10-13 2019 (2019):486-486,
https://hdl.handle.net/21.15107/rcub_veterinar_2640 .

TY  - CONF
AU  - Ćupić Miladinović, Dejana
AU  - Borozan, Sunčica
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Đukić-Ćosić, Danijela
AU  - Ćupić, Vitomir
AU  - Ivanović, Saša
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2647
AB  - The aim of this study was to investigate the influence of vitamin B1 (thiamine) on biochemical changes in the brain tissue of Japanese quail (Coturnix japanica) treated with chlorpyrifos. The following parameters were examined: cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase – BChE), nitrite concentration–NO2- (parameter of oxidative/nitrosative stress), activity of inducible nitric oxide synthase –iNOS, arylesterase –ARE, cyclooxygenase –COX and extracellular signal–regulated kinase –ERK (MAPK). 
The study was conducted on eighty male Japanese quails (2 controls and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil. CPF dissolved in corn oil was administered to three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BW of vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Our studies have shown that CPF significantly inhibited both cholinesterases and ARE in brain, while vitamin B1 increased activity of enzymes in a dose dependent way. Also CPF has led to increase in the concentration of NO2-, activity of iNOS and COX, but after thiamine treatment there has been a decrease of these parameters. There has been a decrease of ERK expression after CPF treatment that demonstrates an increase of apoptotic vulnerability of cells exposed to CPF. 
Overall these results confirm that CPF causes oxidative/nitrosative stress and apoptosis, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".
PB  - Beograd : Serbian Society of Toxicology
C3  - 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018
T1  - The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos
SP  - 152
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2647
ER  - 

@conference{
author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Peković, Sanja and Dacić, Sanja and Đukić-Ćosić, Danijela and Ćupić, Vitomir and Ivanović, Saša",
year = "2018",
abstract = "The aim of this study was to investigate the influence of vitamin B1 (thiamine) on biochemical changes in the brain tissue of Japanese quail (Coturnix japanica) treated with chlorpyrifos. The following parameters were examined: cholinesterase activity (acetylcholinesterase – AChE and butyrylcholinesterase – BChE), nitrite concentration–NO2- (parameter of oxidative/nitrosative stress), activity of inducible nitric oxide synthase –iNOS, arylesterase –ARE, cyclooxygenase –COX and extracellular signal–regulated kinase –ERK (MAPK). 
The study was conducted on eighty male Japanese quails (2 controls and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil. CPF dissolved in corn oil was administered to three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BW of vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Our studies have shown that CPF significantly inhibited both cholinesterases and ARE in brain, while vitamin B1 increased activity of enzymes in a dose dependent way. Also CPF has led to increase in the concentration of NO2-, activity of iNOS and COX, but after thiamine treatment there has been a decrease of these parameters. There has been a decrease of ERK expression after CPF treatment that demonstrates an increase of apoptotic vulnerability of cells exposed to CPF. 
Overall these results confirm that CPF causes oxidative/nitrosative stress and apoptosis, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".",
publisher = "Beograd : Serbian Society of Toxicology",
journal = "10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018",
title = "The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos",
pages = "152-153",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2647"
}

Ćupić Miladinović, D., Borozan, S., Peković, S., Dacić, S., Đukić-Ćosić, D., Ćupić, V.,& Ivanović, S.. (2018). The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos. in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018
Beograd : Serbian Society of Toxicology., 152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_2647

Ćupić Miladinović D, Borozan S, Peković S, Dacić S, Đukić-Ćosić D, Ćupić V, Ivanović S. The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos. in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018. 2018;:152-153.
https://hdl.handle.net/21.15107/rcub_veterinar_2647 .

Ćupić Miladinović, Dejana, Borozan, Sunčica, Peković, Sanja, Dacić, Sanja, Đukić-Ćosić, Danijela, Ćupić, Vitomir, Ivanović, Saša, "The effect of thiamine on activity of enzymes (with a special emphasis on MAPK) in the brain of Japanese quails treated with chlorpyrifos" in 10th Congress of Toxicology in Developing Countries (CTDC 10) and the 12th Serbian Congress of Toxicology, Belgrade, April 18-21, 2018 (2018):152-153,
https://hdl.handle.net/21.15107/rcub_veterinar_2647 .