TY  - JOUR
AU  - Babić, Milijana
AU  - Pajić, Marija
AU  - Radinović, Miodrag
AU  - Boboš, Stanko
AU  - Bulajić, Snežana
AU  - Nikolić, Aleksandra
AU  - Velebit, Branko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1784
AB  - The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Foodborne Pathogens and Disease
T1  - Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk
VL  - 16
IS  - 4
SP  - 282
EP  - 289
DO  - 10.1089/fpd.2018.2544
ER  - 

@article{
author = "Babić, Milijana and Pajić, Marija and Radinović, Miodrag and Boboš, Stanko and Bulajić, Snežana and Nikolić, Aleksandra and Velebit, Branko",
year = "2019",
abstract = "The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Foodborne Pathogens and Disease",
title = "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk",
volume = "16",
number = "4",
pages = "282-289",
doi = "10.1089/fpd.2018.2544"
}

Babić, M., Pajić, M., Radinović, M., Boboš, S., Bulajić, S., Nikolić, A.,& Velebit, B.. (2019). Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease
Mary Ann Liebert, Inc, New Rochelle., 16(4), 282-289.
https://doi.org/10.1089/fpd.2018.2544

Babić M, Pajić M, Radinović M, Boboš S, Bulajić S, Nikolić A, Velebit B. Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease. 2019;16(4):282-289.
doi:10.1089/fpd.2018.2544 .

Babić, Milijana, Pajić, Marija, Radinović, Miodrag, Boboš, Stanko, Bulajić, Snežana, Nikolić, Aleksandra, Velebit, Branko, "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk" in Foodborne Pathogens and Disease, 16, no. 4 (2019):282-289,
https://doi.org/10.1089/fpd.2018.2544 . .

TY  - JOUR
AU  - Pajić, Marija
AU  - Boboš, Stanko
AU  - Velebit, Branko
AU  - Rašić, Zoran
AU  - Katić, Vera
AU  - Radinović, Miodrag
AU  - Nikolić, Aleksandra
AU  - Simonović, Dušan
AU  - Babić, Milijana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1355
AB  - Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.
AB  - Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows
T1  - Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji
VL  - 66
IS  - 4
SP  - 466
EP  - 477
DO  - 10.1515/acve-2016-0040
ER  - 

@article{
author = "Pajić, Marija and Boboš, Stanko and Velebit, Branko and Rašić, Zoran and Katić, Vera and Radinović, Miodrag and Nikolić, Aleksandra and Simonović, Dušan and Babić, Milijana",
year = "2016",
abstract = "Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms., Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows, Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji",
volume = "66",
number = "4",
pages = "466-477",
doi = "10.1515/acve-2016-0040"
}

Pajić, M., Boboš, S., Velebit, B., Rašić, Z., Katić, V., Radinović, M., Nikolić, A., Simonović, D.,& Babić, M.. (2016). Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 466-477.
https://doi.org/10.1515/acve-2016-0040

Pajić M, Boboš S, Velebit B, Rašić Z, Katić V, Radinović M, Nikolić A, Simonović D, Babić M. Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd. 2016;66(4):466-477.
doi:10.1515/acve-2016-0040 .

Pajić, Marija, Boboš, Stanko, Velebit, Branko, Rašić, Zoran, Katić, Vera, Radinović, Miodrag, Nikolić, Aleksandra, Simonović, Dušan, Babić, Milijana, "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows" in Acta Veterinaria-Beograd, 66, no. 4 (2016):466-477,
https://doi.org/10.1515/acve-2016-0040 . .