TY  - CONF
AU  - Marjanović, Đorđe S.
AU  - Rajković, Milan
AU  - Neveu, Cédric
AU  - Trailović, Saša
AU  - Charvet, Claude
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3417
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.
C3  - 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021
T1  - Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3417
ER  - 

@conference{
author = "Marjanović, Đorđe S. and Rajković, Milan and Neveu, Cédric and Trailović, Saša and Charvet, Claude",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemical of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However the antiparasitic mode of action of carvacrol is poorly understood so far. Here, the objective of the work was to characterize and investigate the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptors (AChRs) function both in vivo with the use of worm neuro-muscular flap preparations and in vitro with elecrophysiology on nAChRs expressed in Xenopus oocytes. Strikingly, we observed that carvacrol fully abolished Parascaris sp. muscle contractions elicited by acetylcholine. Conversely, carvacrol inhibited acetylcholine-induced currents on both the nicotine-sentitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that the body muscle flap preparation is a tractable approach to investigate the pharmacology of Parascaris sp. neuro-muscular system. Our results suggest an intriguing mode of action for carvacrol being a potent antagonist of muscle nAChRs of Parascaris sp. worms which may account for its antiparasitic potency.",
journal = "28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021",
title = "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3417"
}

Marjanović, Đ. S., Rajković, M., Neveu, C., Trailović, S.,& Charvet, C.. (2021). Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021.
https://hdl.handle.net/21.15107/rcub_veterinar_3417

Marjanović ĐS, Rajković M, Neveu C, Trailović S, Charvet C. Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp.. in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021. 2021;.
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

Marjanović, Đorđe S., Rajković, Milan, Neveu, Cédric, Trailović, Saša, Charvet, Claude, "Antagonistic effect of carvacrol on nicotinic acetylcholine receptors from the horse parasite Parascaris sp." in 28-th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP 2021), Dublin, 2021 (2021),
https://hdl.handle.net/21.15107/rcub_veterinar_3417 .

TY  - JOUR
AU  - Trailović, Saša M.
AU  - Rajković, Milan
AU  - Marjanović, Đorđe
AU  - Neveu, Cédric
AU  - Charvet, Claude L.
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2232
AB  - Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.
T2  - Pharmaceuticals
T1  - Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors
VL  - 14
IS  - 6
SP  - 505
DO  - 10.3390/ph14060505
ER  - 

@article{
author = "Trailović, Saša M. and Rajković, Milan and Marjanović, Đorđe and Neveu, Cédric and Charvet, Claude L.",
year = "2021",
abstract = "Parascaris sp. is the only ascarid parasitic nematode in equids and one of the most threatening infectious organisms in horses. Only a limited number of compounds are available for treatment of horse helminthiasis, and Parascaris sp. worms have developed resistance to the three major anthelmintic families. In order to overcome the appearance of resistance, there is an urgent need for new therapeutic strategies. The active ingredients of herbal essential oils are potentially effective antiparasitic drugs. Carvacrol is one of the principal chemicals of essential oil from Origanum, Thymus, Coridothymus, Thymbra, Satureja and Lippia herbs. However, the antiparasitic mode of action of carvacrol is poorly understood. Here, the objective of the work was to characterize the activity of carvacrol on Parascaris sp. nicotinic acetylcholine receptor (nAChR) function both in vivo with the use of worm neuromuscular flap preparations and in vitro with two-electrode voltage-clamp electrophysiology on nAChRs expressed in Xenopus oocytes. We developed a neuromuscular contraction assay for Parascaris body flaps and obtained acetylcholine concentration-dependent contraction responses. Strikingly, we observed that 300 µM carvacrol fully and irreversibly abolished Parascaris sp. muscle contractions elicited by acetylcholine. Similarly, carvacrol antagonized acetylcholine-induced currents from both the nicotine-sensitive AChR and the morantel-sensitive AChR subtypes. Thus, we show for the first time that body muscle flap preparation is a tractable approach to investigating the pharmacology of Parascaris sp. neuromuscular system. Our results suggest an intriguing mode of action for carvacrol, being a potent antagonist of muscle nAChRs of Parascaris sp. worms, which may account for its antiparasitic potency.",
journal = "Pharmaceuticals",
title = "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors",
volume = "14",
number = "6",
pages = "505",
doi = "10.3390/ph14060505"
}

Trailović, S. M., Rajković, M., Marjanović, Đ., Neveu, C.,& Charvet, C. L.. (2021). Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals, 14(6), 505.
https://doi.org/10.3390/ph14060505

Trailović SM, Rajković M, Marjanović Đ, Neveu C, Charvet CL. Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. in Pharmaceuticals. 2021;14(6):505.
doi:10.3390/ph14060505 .

Trailović, Saša M., Rajković, Milan, Marjanović, Đorđe, Neveu, Cédric, Charvet, Claude L., "Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors" in Pharmaceuticals, 14, no. 6 (2021):505,
https://doi.org/10.3390/ph14060505 . .