TY  - THES
AU  - Janjić, Filip
PY  - 2021
UR  - https://uvidok.rcub.bg.ac.rs/bitstream/handle/123456789/4288/Referat.pdf
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8418
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24652/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=52984585
UR  - https://nardus.mpn.gov.rs/handle/123456789/18779
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2951
AB  - Babezioza pasa, uzrokovana infekcijom protozoom Babesia canis, sezonska je bolest na području Beograda, glavnog grada Republike Srbije. Sezone pojave oboljenja se poklapaju sa aktivnošću vektora krpelja Dermacentor reticulatus. Period inkubacije varira od četiri do 21 dan, a kliničku sliku karakteriše sistemska akutna inflamacija ili u težim slučajevima sindrom sistemskog inflamatornog odgovora (eng. Systemic Inflammatory Response Syndrome – SIRS). Povezanost klimatskih i epidemioloških faktora koja bi precizno pokazala i okarakterisala sezone u kojima se ovo oboljenje javlja na teritoriji Beograda, do sada nije ispitana. Sa kliničko-epidemiološkog aspekta, nameće se pitanje, da li je pojava SIRS-a vezana za pojedine sezone, odnosno da li postoje razlike u intezitetu odgovora akutne faze kod pasa obolelih u različitim sezonama. Hipoteza ove studije bila je da je tokom prolećne sezone, u kojoj je učestalost pojave pasa akutno inficiranih B. canis najveća, odgovor akutne faze najintenzivniji, odnosno da će reaktanti akutne faze pokazati najveće promene, a da će broj pasa sa SIRS-om, biti najzastupljeniji.Prvi deo studije obuhvatio je ispitivanje učestalosti pojave oboljenja na teritoriji Beograda tokom četiri godine (2013–2016), u različitim sezonama. Ispitan je kratkoročni uticaj (dve nedelje) i dugoročni uticaj meteoroloških parametara (godinu dana) koji su prethodili pojavi 872 zabeležena slučaja babezioze pasa. Drugi deo studije uključio je 66 pasa, sa akutnom infekcijom B. canis tokom 2017–2019. godine, od kojih je prikupljan višak pune krvi i seruma koji su preostali posle dijagnostike oboljenja. Rutinskim laboratorijskim testovima su izvedene hematološke i biohemijske analize, dok je elektroforeza proteina izvedena na agaroznom gelu. Parazitemija je određena komercijalnom metodom lančane reakcije polimeraze, dok je prisustvo IgG antitela protiv B. canis ispitano komercijalnim serološkim testom. Reaktanti/proteini akutne faze određeni su sledećim testovima: koncentracija serum amiloida A komercijalnom ELISA-om (eng. Enzyme Linked Immunosorbent Assay), koncentracija ceruloplazmina i aktivnost paraoksonaze 1 spektrofotometrijski, dok je za koncentraciju fibrinogena primenjen metod zagrevanja uz precipitaciju proteina. Klasifikacija pasa na SIRS pozitivne i negativne izvedena je prema odgovarajućim kliničkim i laboratorijskim promenama, dok su indeksi reaktanata akutne faze (IRAF) dobijeni računskim putem. Razlike između varijabli ispitane su pomoću Kruskal-Wallis, Mann-Whitney U testa i Chi-square testa. Veze između varijabli kvantifikovane su Spirmanovim koeficijentima korelacije odnosno logističkom regresionom analizom.Rezultati analize distribucije pojave babezioze pasa omogućavaju podelu kalendarske godine na četiri sezone: januar–februar, februar–maj, jun–septembar i oktobar–decembar. Broj dijagnostikovanih slučajeva pokazuje bimodalnu distribuciju sa najvećim brojem tokom kasne zime i proleća i nešto manje tokom jeseni. Pokazano je da su u sezoni sa najvećim brojem slučajeva (februar–maj) kratkoročni „optimalni“ uslovi za pojavu babezioze pasa temperatura (3–16) ˚C pri vlažnosti vazduha (53–87) %...
AB  - Canine babesiosis, caused by infection with a protozoan Babesia canis, is a seasonal disease in the area of Belgrade, the capital of the Republic of Serbia. The seasons of the disease coincide with the activity of the vector Dermacentor reticulatus. The disease incubation period varies between 4 and 21 days, and the clinical picture is characterized by systemic acute inflammation or, in severe cases, Systemic Inflammatory Response Syndrome (SIRS). A connection between meteorological parameters and epidemiological factors that could help characterize the seasons in which this disease occurs in the region of Belgrade, has not been investigated previously. From the clinical-epidemiological aspect, there is a question whether the occurrence of SIRS is restricted to some seasons, or, in other words, whether there are differences in the intensity of the acute phase response in dogs infected in different seasons. This study hypothesized that during the spring season, when the frequency of dogs acutely infected with B. canis is the highest, the acute phase response is also the most intense, with acute-phase reactants showing the greatest changes, and the fraction of dogs with SIRS reaching its maximum.The first part of the study investigated the disease frequency in the Belgrade region in different seasons during four years (2013–2016) when a total of 872 were recorded. A short-term influence (two weeks prior) and long-term influence of meteorological parameters (up to one year prior) were analyzed.The second part of the study included 66 dogs with acute B. canis infection diagnosed between 2017 and 2019. Laboratory analyses were conducted using the excess of the whole blood and serum that remained after the diagnosis of the disease. Hematological and biochemical analyses were performed by routine laboratory tests, while agarose gel was used for electrophoretic analysis of proteins. Parasitemia was measured by a polymerase chain reaction method in a commercial laboratory. The presence of IgG antibodies against B. canis was tested by a commercial serological test. The following techniques were used for analyses of acute-phase proteins: commercial Enzyme Linked Immunosorbent Assay for serum amyloid A concentration, spectrophotometry for ceruloplasmin concentration and paraoxonase 1 activity, and heat denaturation method for fibrinogen. The dogs were classified as SIRS positive or negative according to a predefined set of clinical and laboratory changes. The indexes of acute phase reactants (IRAF) were calculated. The significance of the differences between the variables was tested using the Kruskal-Wallis, Mann-Whitney U, and Chi-square tests. The relationships between variables were quantified by Spearman correlation coefficients and logistic regression analyses.The number of diagnosed canine babesiosis cases showed a bimodal distribution wherein four seasons of the year can be distinguished: January–February, February–May, June–September and October–December. The highest number of the cases was recorded during late winter and spring, i.e., in the February–May season, and the other, less prominent maximum, occurred during autumn. In the season with the highest number of cases, from February to May, short-term (two weeks prior) "optimal" conditions for the occurrence of dog babesiosis were temperatures (3–16) ˚C and relative humidity (53–87) %...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Odgovor akutne faze kod pasa obolelih od babezioze tokom različitih godišnjih doba i veza pojave akutnog oboljenja sa meteorološkim parametrima
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18779
ER  - 

@phdthesis{
author = "Janjić, Filip",
year = "2021",
abstract = "Babezioza pasa, uzrokovana infekcijom protozoom Babesia canis, sezonska je bolest na području Beograda, glavnog grada Republike Srbije. Sezone pojave oboljenja se poklapaju sa aktivnošću vektora krpelja Dermacentor reticulatus. Period inkubacije varira od četiri do 21 dan, a kliničku sliku karakteriše sistemska akutna inflamacija ili u težim slučajevima sindrom sistemskog inflamatornog odgovora (eng. Systemic Inflammatory Response Syndrome – SIRS). Povezanost klimatskih i epidemioloških faktora koja bi precizno pokazala i okarakterisala sezone u kojima se ovo oboljenje javlja na teritoriji Beograda, do sada nije ispitana. Sa kliničko-epidemiološkog aspekta, nameće se pitanje, da li je pojava SIRS-a vezana za pojedine sezone, odnosno da li postoje razlike u intezitetu odgovora akutne faze kod pasa obolelih u različitim sezonama. Hipoteza ove studije bila je da je tokom prolećne sezone, u kojoj je učestalost pojave pasa akutno inficiranih B. canis najveća, odgovor akutne faze najintenzivniji, odnosno da će reaktanti akutne faze pokazati najveće promene, a da će broj pasa sa SIRS-om, biti najzastupljeniji.Prvi deo studije obuhvatio je ispitivanje učestalosti pojave oboljenja na teritoriji Beograda tokom četiri godine (2013–2016), u različitim sezonama. Ispitan je kratkoročni uticaj (dve nedelje) i dugoročni uticaj meteoroloških parametara (godinu dana) koji su prethodili pojavi 872 zabeležena slučaja babezioze pasa. Drugi deo studije uključio je 66 pasa, sa akutnom infekcijom B. canis tokom 2017–2019. godine, od kojih je prikupljan višak pune krvi i seruma koji su preostali posle dijagnostike oboljenja. Rutinskim laboratorijskim testovima su izvedene hematološke i biohemijske analize, dok je elektroforeza proteina izvedena na agaroznom gelu. Parazitemija je određena komercijalnom metodom lančane reakcije polimeraze, dok je prisustvo IgG antitela protiv B. canis ispitano komercijalnim serološkim testom. Reaktanti/proteini akutne faze određeni su sledećim testovima: koncentracija serum amiloida A komercijalnom ELISA-om (eng. Enzyme Linked Immunosorbent Assay), koncentracija ceruloplazmina i aktivnost paraoksonaze 1 spektrofotometrijski, dok je za koncentraciju fibrinogena primenjen metod zagrevanja uz precipitaciju proteina. Klasifikacija pasa na SIRS pozitivne i negativne izvedena je prema odgovarajućim kliničkim i laboratorijskim promenama, dok su indeksi reaktanata akutne faze (IRAF) dobijeni računskim putem. Razlike između varijabli ispitane su pomoću Kruskal-Wallis, Mann-Whitney U testa i Chi-square testa. Veze između varijabli kvantifikovane su Spirmanovim koeficijentima korelacije odnosno logističkom regresionom analizom.Rezultati analize distribucije pojave babezioze pasa omogućavaju podelu kalendarske godine na četiri sezone: januar–februar, februar–maj, jun–septembar i oktobar–decembar. Broj dijagnostikovanih slučajeva pokazuje bimodalnu distribuciju sa najvećim brojem tokom kasne zime i proleća i nešto manje tokom jeseni. Pokazano je da su u sezoni sa najvećim brojem slučajeva (februar–maj) kratkoročni „optimalni“ uslovi za pojavu babezioze pasa temperatura (3–16) ˚C pri vlažnosti vazduha (53–87) %..., Canine babesiosis, caused by infection with a protozoan Babesia canis, is a seasonal disease in the area of Belgrade, the capital of the Republic of Serbia. The seasons of the disease coincide with the activity of the vector Dermacentor reticulatus. The disease incubation period varies between 4 and 21 days, and the clinical picture is characterized by systemic acute inflammation or, in severe cases, Systemic Inflammatory Response Syndrome (SIRS). A connection between meteorological parameters and epidemiological factors that could help characterize the seasons in which this disease occurs in the region of Belgrade, has not been investigated previously. From the clinical-epidemiological aspect, there is a question whether the occurrence of SIRS is restricted to some seasons, or, in other words, whether there are differences in the intensity of the acute phase response in dogs infected in different seasons. This study hypothesized that during the spring season, when the frequency of dogs acutely infected with B. canis is the highest, the acute phase response is also the most intense, with acute-phase reactants showing the greatest changes, and the fraction of dogs with SIRS reaching its maximum.The first part of the study investigated the disease frequency in the Belgrade region in different seasons during four years (2013–2016) when a total of 872 were recorded. A short-term influence (two weeks prior) and long-term influence of meteorological parameters (up to one year prior) were analyzed.The second part of the study included 66 dogs with acute B. canis infection diagnosed between 2017 and 2019. Laboratory analyses were conducted using the excess of the whole blood and serum that remained after the diagnosis of the disease. Hematological and biochemical analyses were performed by routine laboratory tests, while agarose gel was used for electrophoretic analysis of proteins. Parasitemia was measured by a polymerase chain reaction method in a commercial laboratory. The presence of IgG antibodies against B. canis was tested by a commercial serological test. The following techniques were used for analyses of acute-phase proteins: commercial Enzyme Linked Immunosorbent Assay for serum amyloid A concentration, spectrophotometry for ceruloplasmin concentration and paraoxonase 1 activity, and heat denaturation method for fibrinogen. The dogs were classified as SIRS positive or negative according to a predefined set of clinical and laboratory changes. The indexes of acute phase reactants (IRAF) were calculated. The significance of the differences between the variables was tested using the Kruskal-Wallis, Mann-Whitney U, and Chi-square tests. The relationships between variables were quantified by Spearman correlation coefficients and logistic regression analyses.The number of diagnosed canine babesiosis cases showed a bimodal distribution wherein four seasons of the year can be distinguished: January–February, February–May, June–September and October–December. The highest number of the cases was recorded during late winter and spring, i.e., in the February–May season, and the other, less prominent maximum, occurred during autumn. In the season with the highest number of cases, from February to May, short-term (two weeks prior) "optimal" conditions for the occurrence of dog babesiosis were temperatures (3–16) ˚C and relative humidity (53–87) %...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Odgovor akutne faze kod pasa obolelih od babezioze tokom različitih godišnjih doba i veza pojave akutnog oboljenja sa meteorološkim parametrima",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18779"
}

Janjić, F.. (2021). Odgovor akutne faze kod pasa obolelih od babezioze tokom različitih godišnjih doba i veza pojave akutnog oboljenja sa meteorološkim parametrima. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_18779

Janjić F. Odgovor akutne faze kod pasa obolelih od babezioze tokom različitih godišnjih doba i veza pojave akutnog oboljenja sa meteorološkim parametrima. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18779 .

Janjić, Filip, "Odgovor akutne faze kod pasa obolelih od babezioze tokom različitih godišnjih doba i veza pojave akutnog oboljenja sa meteorološkim parametrima" (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18779 .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Kalagasidis-Krusić, Melina
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1591
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
VL  - 140
SP  - 11
EP  - 20
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Kalagasidis-Krusić, Melina and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
volume = "140",
pages = "11-20",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Kalagasidis-Krusić, M., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Kalagasidis-Krusić M, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Kalagasidis-Krusić, Melina, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Krušić, Melina Kalagasidis
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1847
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 °C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier
T2  - The Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
VL  - 140
SP  - 11
EP  - 20
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Krušić, Melina Kalagasidis and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 °C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier",
journal = "The Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
volume = "140",
pages = "11-20",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Krušić, M. K., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in The Journal of Supercritical Fluids
Elsevier., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Krušić MK, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in The Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Krušić, Melina Kalagasidis, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in The Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Aksentijević, Ksenija
AU  - Ašanin, Jelena
AU  - Nišavić, Jakov
AU  - Marković, Maja
AU  - Milanov, Dubravka
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1441
AB  - Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL.
AB  - Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibiotic resistance in bacteria isolated from fish in Serbia
T1  - Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije
VL  - 71
IS  - 1
SP  - 24
EP  - 34
DO  - 10.2298/VETGL170329005A
ER  - 

@article{
author = "Aksentijević, Ksenija and Ašanin, Jelena and Nišavić, Jakov and Marković, Maja and Milanov, Dubravka and Mišić, Dušan",
year = "2017",
abstract = "Introduction. Bacteria isolated from skin, gills, and fish intestines from aquaculture ponds, ornamental fish stores/aquariums and live fish markets were investigated. Materials and Methods. Disk diffusion and E-test were used for susceptibility testing to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor, 3rd and 4th generation cephalosporins, aminoglycosides, colistin, fluoroquinolones, and chloramphenicol. PCR was used to detect resistance genes in the bacterial isolates. Results and Conclusions. Among the total number of bacteria tested, regardless of the genus and species, 56.7% of isolates were found to be sensitive to all antibiotics, 23.1% of isolates were resistant to one or two antibiotics, and 20.2% of isolates were resistant to three and up to 16 antibiotics. In A. hydrophila isolated from a guppy (Poecilia reticulata) sampled in an ornamental fish store aquarium, 16S rRNA methyltransferase was confirmed by finding the rmtB gene. Pseudomonas isolates showing phenotypic resistance to carbapenems, ureidopenicillins with or without ß-lactamase inhibitor and 3rd and 4th generation cephalosporins were tested and found negative for different resistance genes by PCR (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9 and the AmpC group, as well as single genes, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Based on E-test results, three Pseudomonas isolates from common carp (Cyprinus carpio) were found to be resistant to colistin with MIC values of 4 μg/mL., Uvod. U ovom ispitivanju izolovane su bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Materijal i metode. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, cefalosporine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije vršeno je primenom metode PCR. Rezultati i zaključak. Posmatrano na ukupan broj ispitanih sojeva, bez obzira na rod i vrstu bakterija, 56,7% sojeva je osetljivo na sve antibiotike, 23,1% sojeva je rezistentno na 1 do 2 antibiotikaa 20,2% sojeva je rezistentno na 3 do 16 antibiotika. Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi nalazom gena rmtBpotvrđeno je prisustvo 16S rRNK metiltransferaze odgovornim za rezistenciju na aminoglikozide. Pseudomonas izolati koji su pokazali fenotipsku rezistenciju na karbapeneme, ureidopeniciline sa i bez inhibitora beta-laktamaza, kao i na cefalosporine III i IV generacije, testirani su i bili negativni na sledeće gene (MßL, ESBL, KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni geni, MOXM, CITM, ACCM, EBCM, FOXM, DHAM). Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibiotic resistance in bacteria isolated from fish in Serbia, Otpornost na antibiotike kod bakterija izolovanih od riba iz Srbije",
volume = "71",
number = "1",
pages = "24-34",
doi = "10.2298/VETGL170329005A"
}

Aksentijević, K., Ašanin, J., Nišavić, J., Marković, M., Milanov, D.,& Mišić, D.. (2017). Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 24-34.
https://doi.org/10.2298/VETGL170329005A

Aksentijević K, Ašanin J, Nišavić J, Marković M, Milanov D, Mišić D. Antibiotic resistance in bacteria isolated from fish in Serbia. in Veterinarski Glasnik. 2017;71(1):24-34.
doi:10.2298/VETGL170329005A .

Aksentijević, Ksenija, Ašanin, Jelena, Nišavić, Jakov, Marković, Maja, Milanov, Dubravka, Mišić, Dušan, "Antibiotic resistance in bacteria isolated from fish in Serbia" in Veterinarski Glasnik, 71, no. 1 (2017):24-34,
https://doi.org/10.2298/VETGL170329005A . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1488
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
SP  - 8762649
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
pages = "8762649",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 8762649.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;:8762649.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science (2017):8762649,
https://doi.org/10.1155/2017/8762649 . .

TY  - THES
AU  - Aksentijević, Ksenija
PY  - 2016
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48634895
UR  - http://nardus.mpn.gov.rs/123456789/7560
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/38
AB  - During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL.
AB  - U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments
T1  - Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7560
ER  - 

@phdthesis{
author = "Aksentijević, Ksenija",
year = "2016",
abstract = "During this research, a series of microbiological smears was collected from clinically healthy fish found in different environments (aquaculture ponds, aquariums, and fish markets) has been done. Bacteria which belong to skin microbiome, gills, and fish intestines have been isolated, and their sensitivity to several antibiotics used in veterinary and human practice has been tested. Precise identification of tested strains of bacteria has been performed with PCR method, 16S rRNA gene sequencing and MALDI-TOF. Phenotypical manifestation of resistance to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosporins of the third and fourth generation, aminoglycosides, tetracyclines, colistin, fluoroquinolones and chloramphenicol has been tested using disc diffusion method and E test. Presence of resistant genes, their localization (on chromosome or on mobile genetic elements) has been conducted with PCR method. For strains showing resistance to the antibiotics mentioned above, plasmids have been searched and conjugation of isolated plasmids has been tested. Observing the total number of tested strains in this research, regardless of the genus and species of bacteria, 55% of examined strains were found to be sensitive to all antibiotics, and in 22.8% of strains resistance was observed to 3-16 antibiotics, including antibiotics used exclusively in human medicine (carbapenems, ureidopenicillins, cephalosporins of third and fourth generation). In additional 22.2% of strains the resistance to 1 or 2 antibiotics was recorded, including resistance to antibiotics registered for exclusive use in human medicine (ceftazidime, piperacillin). In A. hydrophila strain isolated from aquarium fish guppy that showed resistance to all 16 antibiotics, a mechanism of resistance has been confirmed by identifying gene rmtB which has been localized on transposon Tn1548 located on conjugal plasmid which belongs to group IncL/M type of replicon. In Pseudomonas strains resistant to carbapenems, ureidopenicillins (with and without inhibitors of beta-lactamase), cephalosprins of third and fourth generation, the genes for carbapenemases MßL, ESBL, OXA and AmpC beta-laktamases (KPC, OXA-23, OXA-24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTX-M-9, OXA-1, OXA-9, group AmpC and specific MOXM, CITM, ACCM, EBCM, FOXM, DHAM) have not been found. Based on results obtained with use of E test, resistence to colistin has been found in 3 strains of Pseudomonas spp. isolated from carp with MIC values of 4 μg/mL., U ovom ispitivanju vršeno je uzorkovanje briseva poreklom od klinički zdravih riba koje su poticale iz različitih sredina (ribnjaci, akvarijumi, riblje pijace). Izvršena je izolacija bakterija koje su sastavni deo mikrobioma kože, škrga i creva riba i ispitivana je osetljivost ovih bakterija na određeni broj antibiotika koji se koriste u veterinarskoj i humanoj medicinskoj praksi. Precizna identifikacija ispitivanih sojeva bakterija vršena je primenom metoda PCR, sekvenciranje gena za 16S rRNK, MALDI-TOF. Primenom disk difuzionog testa i E testa ispitivano je fenotipsko ispoljavanje rezistencije na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, cefalospirine III i IV generacije, aminoglikozide, tetraciklin, kolistin, flurohinolone i hloramfenikol. Prisustvo gena rezistencije, njihova lokalizacija (na hromozomu ili na mobilnim genetičkim elementima) vršena je primenom metode PCR. Kod sojeva koji su ispoljili rezistenciju na nabrojane antibiotike traženi su plazmidi i ispitivana je konjugabilnost izolovanih plazmida. Posmatrano na ukupan broj ispitanih sojeva u ovom istraživanju, bez obzira na rod i vrstu bakterija, ukupno je nađeno 55% sojeva koji su bili osetljivi na sve antibiotike, kod 22,8% sojeva nađena je rezistencija na 3 do 16 antibiotika uključujući i antibiotike koji se koriste isključivo kod ljudi (karbapenemi, ureidopenicilini, cefalosporini III i IV generacije). Ukupno 22,2% sojeva bilo je rezistentno na 1 do 2 antibiotika, mada je i među tim sojevima bilo onih koji su bili rezistentni na antibiotike registrovane samo za upotrebu kod ljudi (ceftazidim, piperacilin). Kod soja A. hydrophila izolovanom iz akvarijumske ribice gupi potvrđen je mehanizam rezistencije nalazom gena rmtB koji je bio lokalizovan na transpozonu Tn1548 smeštenom na konjugabilnom plazmidu koji je po tipu replikona bio kategorisan u grupu IncL/M. Kod sojeva Pseudomonas koji su bili rezistentni na karbapeneme, ureidopeniciline sa i bez inhibitora betalaktamaza, kao i na cefalosporine III i IV generacije, nisu nađeni geni za, karbapenemaze, MßL, ESBL, OXA i AmpC beta-laktamaze (KPC, OXA-23, OXA- 24, OXA-40, OXA-58, VIM, IMP, SPM, GIM, NDM, TEM, SHV, CTX-M-1, CTXM- 9, OXA-1, OXA-9, AmpC grupni kao i pojedinačni-MOXM, CITM, ACCM, EBCM, FOXM, DHAM).Na osnovu dobijenih rezultata primenom E testa, kod 3 soja iz roda Pseudomonas izolovanih od šarana nađena je rezistencija na kolistin sa dobijenim vrednostima MIK 4 μg/mL.",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments, Ispitivanje rezistencije na antibiotike kod sojeva bakterija izolovanih od riba poreklom iz različitih sredina",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7560"
}

Aksentijević, K.. (2016). Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_7560

Aksentijević K. Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

Aksentijević, Ksenija, "Study of antibiotics resistance in bacterial strains isolated from fish collected from different environments" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7560 .

TY  - JOUR
AU  - Fanovich, M. A.
AU  - Ivanović, J.
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Jaeger, Philip
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1369
AB  - Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2
VL  - 58
SP  - 204
EP  - 212
DO  - 10.1016/j.msec.2015.08.024
ER  - 

@article{
author = "Fanovich, M. A. and Ivanović, J. and Žižović, Irena and Mišić, Dušan and Jaeger, Philip",
year = "2016",
abstract = "Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2",
volume = "58",
pages = "204-212",
doi = "10.1016/j.msec.2015.08.024"
}

Fanovich, M. A., Ivanović, J., Žižović, I., Mišić, D.,& Jaeger, P.. (2016). Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 58, 204-212.
https://doi.org/10.1016/j.msec.2015.08.024

Fanovich MA, Ivanović J, Žižović I, Mišić D, Jaeger P. Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications. 2016;58:204-212.
doi:10.1016/j.msec.2015.08.024 .

Fanovich, M. A., Ivanović, J., Žižović, Irena, Mišić, Dušan, Jaeger, Philip, "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2" in Materials Science & Engineering C-Materials for Biological Applications, 58 (2016):204-212,
https://doi.org/10.1016/j.msec.2015.08.024 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lunter, Dominique Jasmin
AU  - Daniels, Rolf
AU  - Pantelić, Ivana
AU  - Tasic-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Arsić, Ivana
AU  - Savić, Snežana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1390
AB  - Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Development and Technology
T1  - Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
VL  - 21
IS  - 5
SP  - 563
EP  - 575
DO  - 10.3109/10837450.2015.1026606
ER  - 

@article{
author = "Žugić, Ana R. and Lunter, Dominique Jasmin and Daniels, Rolf and Pantelić, Ivana and Tasic-Kostov, Marija and Tadić, Vanja M. and Mišić, Dušan and Arsić, Ivana and Savić, Snežana",
year = "2016",
abstract = "Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Development and Technology",
title = "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product",
volume = "21",
number = "5",
pages = "563-575",
doi = "10.3109/10837450.2015.1026606"
}

Žugić, A. R., Lunter, D. J., Daniels, R., Pantelić, I., Tasic-Kostov, M., Tadić, V. M., Mišić, D., Arsić, I.,& Savić, S.. (2016). Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology
Taylor & Francis Ltd, Abingdon., 21(5), 563-575.
https://doi.org/10.3109/10837450.2015.1026606

Žugić AR, Lunter DJ, Daniels R, Pantelić I, Tasic-Kostov M, Tadić VM, Mišić D, Arsić I, Savić S. Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology. 2016;21(5):563-575.
doi:10.3109/10837450.2015.1026606 .

Žugić, Ana R., Lunter, Dominique Jasmin, Daniels, Rolf, Pantelić, Ivana, Tasic-Kostov, Marija, Tadić, Vanja M., Mišić, Dušan, Arsić, Ivana, Savić, Snežana, "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product" in Pharmaceutical Development and Technology, 21, no. 5 (2016):563-575,
https://doi.org/10.3109/10837450.2015.1026606 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Aksentijević, Ksenija
AU  - Stojanović, Dusica B.
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1405
AB  - Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Application of cellulose acetate for controlled release of thymol
VL  - 147
SP  - 344
EP  - 353
DO  - 10.1016/j.carbpol.2016.03.093
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Aksentijević, Ksenija and Stojanović, Dusica B. and Ivanović, Jasna and Žižović, Irena",
year = "2016",
abstract = "Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Application of cellulose acetate for controlled release of thymol",
volume = "147",
pages = "344-353",
doi = "10.1016/j.carbpol.2016.03.093"
}

Milovanović, S., Marković, D., Aksentijević, K., Stojanović, D. B., Ivanović, J.,& Žižović, I.. (2016). Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 147, 344-353.
https://doi.org/10.1016/j.carbpol.2016.03.093

Milovanović S, Marković D, Aksentijević K, Stojanović DB, Ivanović J, Žižović I. Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers. 2016;147:344-353.
doi:10.1016/j.carbpol.2016.03.093 .

Milovanović, Stoja, Marković, Darka, Aksentijević, Ksenija, Stojanović, Dusica B., Ivanović, Jasna, Žižović, Irena, "Application of cellulose acetate for controlled release of thymol" in Carbohydrate Polymers, 147 (2016):344-353,
https://doi.org/10.1016/j.carbpol.2016.03.093 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica Z.
AU  - Tasic-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Arsić, Ivana
AU  - Mišić, Dušan
AU  - Petrović, Slobodan D.
AU  - Savić, Snežana D.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1216
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
VL  - 69
IS  - 6
SP  - 703
EP  - 712
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica Z. and Tasic-Kostov, Marija and Tadić, Vanja M. and Arsić, Ivana and Mišić, Dušan and Petrović, Slobodan D. and Savić, Snežana D.",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
volume = "69",
number = "6",
pages = "703-712",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M. Z., Tasic-Kostov, M., Tadić, V. M., Arsić, I., Mišić, D., Petrović, S. D.,& Savić, S. D.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić MZ, Tasic-Kostov M, Tadić VM, Arsić I, Mišić D, Petrović SD, Savić SD. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska Industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica Z., Tasic-Kostov, Marija, Tadić, Vanja M., Arsić, Ivana, Mišić, Dušan, Petrović, Slobodan D., Savić, Snežana D., "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska Industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Roj, Edward
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Arsić, Ivana
AU  - Dobrzynska-Inger, Agnieszka
AU  - Kostrzewa, Dorota
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1230
AB  - Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Open Chemistry
T1  - Supercritical carbon dioxide hops extracts with antimicrobial properties
VL  - 13
IS  - 1
SP  - 1157
EP  - 1171
DO  - 10.1515/chem-2015-0131
ER  - 

@article{
author = "Roj, Edward and Tadić, Vanja M. and Mišić, Dušan and Žižović, Irena and Arsić, Ivana and Dobrzynska-Inger, Agnieszka and Kostrzewa, Dorota",
year = "2015",
abstract = "Extracts obtained from hops (Humulus lupulus L., Cannabaceae) by supercritical fluid extraction (SFE), SFE followed by isomerization, as well as by conventional technique, were investigated for their chemical composition and antibacterial activity against selected foodborne pathogens and microorganisms capable to cause the food spoilage. The antibacterial activity of the extracts was compared with the antibacterial activity of xanthohumol, compound known for its broad pharmacological properties, isolated from the raw material remained after the SFE. Xanthohumol (XH, 96%) proved to posses the most prominent activity against all the tested strains, with the MIC values ranged between 2.5 and 20 mu g mL(-1). Supercritical hops extract and potassium isomerized supercritical hops extract showed strong antibacterial activity against the tested strains as well. Escherichia coli was not affected by the extracts, meaning that their oral admission would not cause the same problem as antibiotic application in intestinal flora. The chemical composition of the investigated hops extracts was analysed by GC-MS. Contents of a-acids, beta-acids, iso-alpha-acids and xanthohumol in the samples were determined by HPLC.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Open Chemistry",
title = "Supercritical carbon dioxide hops extracts with antimicrobial properties",
volume = "13",
number = "1",
pages = "1157-1171",
doi = "10.1515/chem-2015-0131"
}

Roj, E., Tadić, V. M., Mišić, D., Žižović, I., Arsić, I., Dobrzynska-Inger, A.,& Kostrzewa, D.. (2015). Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry
De Gruyter Open Ltd, Warsaw., 13(1), 1157-1171.
https://doi.org/10.1515/chem-2015-0131

Roj E, Tadić VM, Mišić D, Žižović I, Arsić I, Dobrzynska-Inger A, Kostrzewa D. Supercritical carbon dioxide hops extracts with antimicrobial properties. in Open Chemistry. 2015;13(1):1157-1171.
doi:10.1515/chem-2015-0131 .

Roj, Edward, Tadić, Vanja M., Mišić, Dušan, Žižović, Irena, Arsić, Ivana, Dobrzynska-Inger, Agnieszka, Kostrzewa, Dorota, "Supercritical carbon dioxide hops extracts with antimicrobial properties" in Open Chemistry, 13, no. 1 (2015):1157-1171,
https://doi.org/10.1515/chem-2015-0131 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Đorđević, Sofija M.
AU  - Tadić, Vanja M.
AU  - Arsić, Ivana
AU  - Mišić, Dušan
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/992
AB  - Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root (Inulae radix) have been used as antiseptics, expectorants, diuretics and bactericidal agents. The aim of this study was to investigate the antimicrobial activity of different Inulae radix isolates against microorganisms that commonly cause skin infections. The essential oil obtained by hydrodistillation (IH-HD) and Sohxlet extracts, ether (IH-SOX-E) and ethanol (IH-SOX-Et) fractions were investigate The isolates were dissolved in DMSO at the concentration of 2560 to 0.625 μg/mL. The antimicrobial activity was tested by using the microdilution method against reference bacterial strains and clinical isolates from skin swabs. IH-SOX-E exhibited a strong antimicrobial activity against all tested strains, IH-HD as well, with the exception of S. aureus, ATCC 25923 (moderate activity), while IH-SOX-Et showed a strong activity only against clinical isolate of C. albicans. The results of our study indicate that elecampane root isolates may be used for incorporation into phytopreparations for topical application in treating skin infections, especially those caused by C. Albicans the most possible sites for interactions with other compounds.
AB  - Oman (Inula helenium L., Asteraceae) je cenjena lekovita biljka, čiji je koren poznat po antiseptičnom ekspektorantnom, diuretičnom i baktericidnom delovanju. Cilj rada bio je ispitivanje antimikrobne aktivnosti različitih izolata korena omana, Inulae radix protiv mikroorganizama koje uobičajeno izazivaju infekcije kože. Ispitivani su etarsko ulje dobijeno hidrodestilacijom (IH-HD) i Sohxlet ekstrakti i to etarska (IH-SOX-E) i etanolna (IH-SOX-Et) frakcija. Izolati su rastvoreni u DMSO u koncentraciji od 2560 do 0,625 μg/mL. Antimikrobna aktivnost ispitivana je mikrodilucionom metodom u bujonu na referentnim bakterijskim sojevima i kliničkim izolatima iz briseva kože. IH-SOX-E ispoljila je jaku antimikrobnu aktivnost prema svim testiranim mikroorganizmima, IH-HD takođe, osim prema Streptococcus aureus ATCC 25923 (umerena aktivnost), a IH-SOX-Et jaku aktivnost pokazala je jedino prema kliničkom izolatu Candida albicans. Rezultati naših ispitivanja pokazuju da se izolati korena omana mogu koristiti za inkorporiranje u fitopreparate namenjene za spoljašnju primenu kod kožnih infekcija, naročito gljivičnih infekcija izazvanih C. albicans.
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Savremene tehnologije
T1  - Antimicrobial activities of elecampane root isolates
T1  - Antimikrobna aktivnost izolata korena omana
VL  - 2
IS  - 2
SP  - 54
EP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_992
ER  - 

@article{
author = "Žugić, Ana R. and Đorđević, Sofija M. and Tadić, Vanja M. and Arsić, Ivana and Mišić, Dušan",
year = "2013",
abstract = "Elecampane (Inula helenium L., Asteraceae), a well-known traditional medicinal herb, and its root (Inulae radix) have been used as antiseptics, expectorants, diuretics and bactericidal agents. The aim of this study was to investigate the antimicrobial activity of different Inulae radix isolates against microorganisms that commonly cause skin infections. The essential oil obtained by hydrodistillation (IH-HD) and Sohxlet extracts, ether (IH-SOX-E) and ethanol (IH-SOX-Et) fractions were investigate The isolates were dissolved in DMSO at the concentration of 2560 to 0.625 μg/mL. The antimicrobial activity was tested by using the microdilution method against reference bacterial strains and clinical isolates from skin swabs. IH-SOX-E exhibited a strong antimicrobial activity against all tested strains, IH-HD as well, with the exception of S. aureus, ATCC 25923 (moderate activity), while IH-SOX-Et showed a strong activity only against clinical isolate of C. albicans. The results of our study indicate that elecampane root isolates may be used for incorporation into phytopreparations for topical application in treating skin infections, especially those caused by C. Albicans the most possible sites for interactions with other compounds., Oman (Inula helenium L., Asteraceae) je cenjena lekovita biljka, čiji je koren poznat po antiseptičnom ekspektorantnom, diuretičnom i baktericidnom delovanju. Cilj rada bio je ispitivanje antimikrobne aktivnosti različitih izolata korena omana, Inulae radix protiv mikroorganizama koje uobičajeno izazivaju infekcije kože. Ispitivani su etarsko ulje dobijeno hidrodestilacijom (IH-HD) i Sohxlet ekstrakti i to etarska (IH-SOX-E) i etanolna (IH-SOX-Et) frakcija. Izolati su rastvoreni u DMSO u koncentraciji od 2560 do 0,625 μg/mL. Antimikrobna aktivnost ispitivana je mikrodilucionom metodom u bujonu na referentnim bakterijskim sojevima i kliničkim izolatima iz briseva kože. IH-SOX-E ispoljila je jaku antimikrobnu aktivnost prema svim testiranim mikroorganizmima, IH-HD takođe, osim prema Streptococcus aureus ATCC 25923 (umerena aktivnost), a IH-SOX-Et jaku aktivnost pokazala je jedino prema kliničkom izolatu Candida albicans. Rezultati naših ispitivanja pokazuju da se izolati korena omana mogu koristiti za inkorporiranje u fitopreparate namenjene za spoljašnju primenu kod kožnih infekcija, naročito gljivičnih infekcija izazvanih C. albicans.",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Savremene tehnologije",
title = "Antimicrobial activities of elecampane root isolates, Antimikrobna aktivnost izolata korena omana",
volume = "2",
number = "2",
pages = "54-58",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_992"
}

Žugić, A. R., Đorđević, S. M., Tadić, V. M., Arsić, I.,& Mišić, D.. (2013). Antimicrobial activities of elecampane root isolates. in Savremene tehnologije
Univerzitet u Nišu - Tehnološki fakultet, Leskovac., 2(2), 54-58.
https://hdl.handle.net/21.15107/rcub_veterinar_992

Žugić AR, Đorđević SM, Tadić VM, Arsić I, Mišić D. Antimicrobial activities of elecampane root isolates. in Savremene tehnologije. 2013;2(2):54-58.
https://hdl.handle.net/21.15107/rcub_veterinar_992 .

Žugić, Ana R., Đorđević, Sofija M., Tadić, Vanja M., Arsić, Ivana, Mišić, Dušan, "Antimicrobial activities of elecampane root isolates" in Savremene tehnologije, 2, no. 2 (2013):54-58,
https://hdl.handle.net/21.15107/rcub_veterinar_992 .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Meyer, Florian
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1015
AB  - Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent
VL  - 76
SP  - 1
EP  - 9
DO  - 10.1016/j.supflu.2013.01.005
ER  - 

@article{
author = "Ivanović, Jasna and Meyer, Florian and Mišić, Dušan and Ašanin, Jelena and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent",
volume = "76",
pages = "1-9",
doi = "10.1016/j.supflu.2013.01.005"
}

Ivanović, J., Meyer, F., Mišić, D., Ašanin, J., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids
Elsevier, Amsterdam., 76, 1-9.
https://doi.org/10.1016/j.supflu.2013.01.005

Ivanović J, Meyer F, Mišić D, Ašanin J, Jaeger P, Žižović I, Eggers R. Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids. 2013;76:1-9.
doi:10.1016/j.supflu.2013.01.005 .

Ivanović, Jasna, Meyer, Florian, Mišić, Dušan, Ašanin, Jelena, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent" in Journal of Supercritical Fluids, 76 (2013):1-9,
https://doi.org/10.1016/j.supflu.2013.01.005 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Dimitrijević-Branković, Suzana
AU  - Mišić, Dušan
AU  - Ristić, Mihailo
AU  - Žižović, Irena
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1019
AB  - For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds
VL  - 5
IS  - 1
SP  - 416
EP  - 423
DO  - 10.1016/j.jff.2012.11.014
ER  - 

@article{
author = "Ivanović, Jasna and Dimitrijević-Branković, Suzana and Mišić, Dušan and Ristić, Mihailo and Žižović, Irena",
year = "2013",
abstract = "For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds",
volume = "5",
number = "1",
pages = "416-423",
doi = "10.1016/j.jff.2012.11.014"
}

Ivanović, J., Dimitrijević-Branković, S., Mišić, D., Ristić, M.,& Žižović, I.. (2013). Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods
Elsevier, Amsterdam., 5(1), 416-423.
https://doi.org/10.1016/j.jff.2012.11.014

Ivanović J, Dimitrijević-Branković S, Mišić D, Ristić M, Žižović I. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods. 2013;5(1):416-423.
doi:10.1016/j.jff.2012.11.014 .

Ivanović, Jasna, Dimitrijević-Branković, Suzana, Mišić, Dušan, Ristić, Mihailo, Žižović, Irena, "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds" in Journal of Functional Foods, 5, no. 1 (2013):416-423,
https://doi.org/10.1016/j.jff.2012.11.014 . .

TY  - JOUR
AU  - Fanovich, M. A.
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Alvarez, M. V.
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1020
AB  - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
VL  - 78
SP  - 42
EP  - 53
DO  - 10.1016/j.supflu.2013.03.017
ER  - 

@article{
author = "Fanovich, M. A. and Ivanović, Jasna and Mišić, Dušan and Alvarez, M. V. and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process",
volume = "78",
pages = "42-53",
doi = "10.1016/j.supflu.2013.03.017"
}

Fanovich, M. A., Ivanović, J., Mišić, D., Alvarez, M. V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 78, 42-53.
https://doi.org/10.1016/j.supflu.2013.03.017

Fanovich MA, Ivanović J, Mišić D, Alvarez MV, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53.
doi:10.1016/j.supflu.2013.03.017 .

Fanovich, M. A., Ivanović, Jasna, Mišić, Dušan, Alvarez, M. V., Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53,
https://doi.org/10.1016/j.supflu.2013.03.017 . .

TY  - JOUR
AU  - Tadić, Vanja M.
AU  - Bojović, Dragica
AU  - Arsić, Ivana
AU  - Đorđević, Sofija
AU  - Aksentijević, Ksenija
AU  - Stamenić, Marko
AU  - Janković, Slobodan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/879
AB  - Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.
PB  - MDPI, Basel
T2  - Molecules
T1  - Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts
VL  - 17
IS  - 3
SP  - 2683
EP  - 2703
DO  - 10.3390/molecules17032683
ER  - 

@article{
author = "Tadić, Vanja M. and Bojović, Dragica and Arsić, Ivana and Đorđević, Sofija and Aksentijević, Ksenija and Stamenić, Marko and Janković, Slobodan",
year = "2012",
abstract = "Sideritis scardica Griseb., Lamiaceae (ironwort, mountain tea), an endemic plant of the Balkan Peninsula, has been used in traditional medicine in the treatment of antimicrobial infections, gastrointestinal complaints, inflammation and rheumatic disorders. This study reports a comparison between conventional (hydrodistillation HD and solvent extraction SE) and alternative (supercritical carbon dioxide SC CO2) extraction methods regarding the qualitative and quantitative composition of the obtained extracts as analyzed by GC and GC-MS techniques and their anitimicrobial activity. Different types of extracts were tested, the essential oil EO obtained by HD, EO-CO2 and AO-CO2 obtained by SC CO2 at different preasures 10 and 30 MPa, at 40 degrees C, respectively, and the fractions A, B, C and D obtained by successive solvent extraction (SE) A: ethanol, B: diethyl ether, C: ethyl acetate and D: n-butanol). While EO was characterized by the presence of the high percentage of oxygenated monoterpenes and sesquiterpenes (30.01 and 25.54%, respectively), the rest of the investigated samples were the most abundant in fatty acids and their esters and diterpenes (from 16.72 to 71.07% for fatty acids and their esters, and from 23.30 to 72.76%, for diterpenes). Microbial susceptibility tests revealed the strong to moderate activity of all investigated extracts against the tested microorganisms (MIC from 40 to 2,560 mu g/mL). Although differences in the chemical compositions determined by GC and GC-MS analysis were established, the displayed antimicrobial activity was similar for the all investigated extracts.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts",
volume = "17",
number = "3",
pages = "2683-2703",
doi = "10.3390/molecules17032683"
}

Tadić, V. M., Bojović, D., Arsić, I., Đorđević, S., Aksentijević, K., Stamenić, M.,& Janković, S.. (2012). Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules
MDPI, Basel., 17(3), 2683-2703.
https://doi.org/10.3390/molecules17032683

Tadić VM, Bojović D, Arsić I, Đorđević S, Aksentijević K, Stamenić M, Janković S. Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts. in Molecules. 2012;17(3):2683-2703.
doi:10.3390/molecules17032683 .

Tadić, Vanja M., Bojović, Dragica, Arsić, Ivana, Đorđević, Sofija, Aksentijević, Ksenija, Stamenić, Marko, Janković, Slobodan, "Chemical and Antimicrobial Evaluation of Supercritical and Conventional Sideritis scardica Griseb., Lamiaceae Extracts" in Molecules, 17, no. 3 (2012):2683-2703,
https://doi.org/10.3390/molecules17032683 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukic-Marković, Jelena
AU  - Petrović, Slobodan D.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/905
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
VL  - 72
SP  - 7
EP  - 14
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukic-Marković, Jelena and Petrović, Slobodan D.",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
volume = "72",
pages = "7-14",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukic-Marković, J.,& Petrović, S. D.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukic-Marković J, Petrović SD. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukic-Marković, Jelena, Petrović, Slobodan D., "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Arsić, Ivana
AU  - Žugić, Ana R.
AU  - Tadić, Vanja M.
AU  - Tasic-Kostov, Marija
AU  - Mišić, Dušan
AU  - Primorac, Marija
AU  - Runjaic-Antić, Dusanka
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/890
AB  - Oleum Hyperici, the oil extract of St. Johns Wort (SJW), is one of the oldest folk remedies, traditionally used in the topical treatment of wounds, bruises, ulcers, cuts, burns, hemorrhoids and also as an antiseptic. Considering the advantageous characteristics of emulsion applications, in the present study we have formulated three O/W creams containing 15% (w/v) of SJW oil extract as an active ingredient. The aim was to estimate dermatological application of the prepared creams for the abovementioned indications. The extracts were prepared according to the prescriptions from traditional medicine, however with different vegetable oils used as an extractant, namely: Olive, palm and sunflower oil. The investigated O/W creams demonstrated significant antiinflammatory effects in an in vivo double-blind randomized study, using a sodium lauryl sulphate test. Both skin parameters assessed in the study (electrical capacitance and erythema index), were restored to the baseline value after a seven-day treatment with the tested creams. Almost all investigated SJW oil extracts and corresponding creams displayed the same antimicrobial activity against the most of the investigated microorganisms with obtained minimal inhibitory concentrations values of 1,280 mu g/mL, 2,560 mu g/mL or >2,560 mu g/mL.
PB  - MDPI, Basel
T2  - Molecules
T1  - Estimation of Dermatological Application of Creams with St. John s Wort Oil Extracts
VL  - 17
IS  - 1
SP  - 275
EP  - 294
DO  - 10.3390/molecules17010275
ER  - 

@article{
author = "Arsić, Ivana and Žugić, Ana R. and Tadić, Vanja M. and Tasic-Kostov, Marija and Mišić, Dušan and Primorac, Marija and Runjaic-Antić, Dusanka",
year = "2012",
abstract = "Oleum Hyperici, the oil extract of St. Johns Wort (SJW), is one of the oldest folk remedies, traditionally used in the topical treatment of wounds, bruises, ulcers, cuts, burns, hemorrhoids and also as an antiseptic. Considering the advantageous characteristics of emulsion applications, in the present study we have formulated three O/W creams containing 15% (w/v) of SJW oil extract as an active ingredient. The aim was to estimate dermatological application of the prepared creams for the abovementioned indications. The extracts were prepared according to the prescriptions from traditional medicine, however with different vegetable oils used as an extractant, namely: Olive, palm and sunflower oil. The investigated O/W creams demonstrated significant antiinflammatory effects in an in vivo double-blind randomized study, using a sodium lauryl sulphate test. Both skin parameters assessed in the study (electrical capacitance and erythema index), were restored to the baseline value after a seven-day treatment with the tested creams. Almost all investigated SJW oil extracts and corresponding creams displayed the same antimicrobial activity against the most of the investigated microorganisms with obtained minimal inhibitory concentrations values of 1,280 mu g/mL, 2,560 mu g/mL or >2,560 mu g/mL.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Estimation of Dermatological Application of Creams with St. John s Wort Oil Extracts",
volume = "17",
number = "1",
pages = "275-294",
doi = "10.3390/molecules17010275"
}

Arsić, I., Žugić, A. R., Tadić, V. M., Tasic-Kostov, M., Mišić, D., Primorac, M.,& Runjaic-Antić, D.. (2012). Estimation of Dermatological Application of Creams with St. John s Wort Oil Extracts. in Molecules
MDPI, Basel., 17(1), 275-294.
https://doi.org/10.3390/molecules17010275

Arsić I, Žugić AR, Tadić VM, Tasic-Kostov M, Mišić D, Primorac M, Runjaic-Antić D. Estimation of Dermatological Application of Creams with St. John s Wort Oil Extracts. in Molecules. 2012;17(1):275-294.
doi:10.3390/molecules17010275 .

Arsić, Ivana, Žugić, Ana R., Tadić, Vanja M., Tasic-Kostov, Marija, Mišić, Dušan, Primorac, Marija, Runjaic-Antić, Dusanka, "Estimation of Dermatological Application of Creams with St. John s Wort Oil Extracts" in Molecules, 17, no. 1 (2012):275-294,
https://doi.org/10.3390/molecules17010275 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Ristić, Mihailo
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/873
AB  - Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates
VL  - 25
IS  - 1
SP  - 110
EP  - 116
DO  - 10.1016/j.foodcont.2011.10.019
ER  - 

@article{
author = "Ivanović, Jasna and Mišić, Dušan and Žižović, Irena and Ristić, Mihailo",
year = "2012",
abstract = "Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates",
volume = "25",
number = "1",
pages = "110-116",
doi = "10.1016/j.foodcont.2011.10.019"
}

Ivanović, J., Mišić, D., Žižović, I.,& Ristić, M.. (2012). In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control
Elsevier Sci Ltd, Oxford., 25(1), 110-116.
https://doi.org/10.1016/j.foodcont.2011.10.019

Ivanović J, Mišić D, Žižović I, Ristić M. In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control. 2012;25(1):110-116.
doi:10.1016/j.foodcont.2011.10.019 .

Ivanović, Jasna, Mišić, Dušan, Žižović, Irena, Ristić, Mihailo, "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates" in Food Control, 25, no. 1 (2012):110-116,
https://doi.org/10.1016/j.foodcont.2011.10.019 . .