Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay
Само за регистроване кориснике
2013
Аутори
Radaković, MilenaStevanović, Jevrosima
Đelić, Ninoslav
Lakić, Nada
Knežević-Vukčević, Jelena
Vukovic-Gacić, Branka
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive o...xygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.
Кључне речи:
Amitraz / apoptosis / catalase / Comet assay / DNA damageИзвор:
Journal of Biosciences, 2013, 38, 1, 53-62Издавач:
- Indian Acad Sciences, Bangalore
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1007/s12038-012-9287-2
ISSN: 0250-5991
PubMed: 23385813
WoS: 000314749100009
Scopus: 2-s2.0-84873715822
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Radaković, Milena AU - Stevanović, Jevrosima AU - Đelić, Ninoslav AU - Lakić, Nada AU - Knežević-Vukčević, Jelena AU - Vukovic-Gacić, Branka AU - Stanimirović, Zoran PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1037 AB - Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis. PB - Indian Acad Sciences, Bangalore T2 - Journal of Biosciences T1 - Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay VL - 38 IS - 1 SP - 53 EP - 62 DO - 10.1007/s12038-012-9287-2 ER -
@article{ author = "Radaković, Milena and Stevanović, Jevrosima and Đelić, Ninoslav and Lakić, Nada and Knežević-Vukčević, Jelena and Vukovic-Gacić, Branka and Stanimirović, Zoran", year = "2013", abstract = "Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.", publisher = "Indian Acad Sciences, Bangalore", journal = "Journal of Biosciences", title = "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay", volume = "38", number = "1", pages = "53-62", doi = "10.1007/s12038-012-9287-2" }
Radaković, M., Stevanović, J., Đelić, N., Lakić, N., Knežević-Vukčević, J., Vukovic-Gacić, B.,& Stanimirović, Z.. (2013). Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences Indian Acad Sciences, Bangalore., 38(1), 53-62. https://doi.org/10.1007/s12038-012-9287-2
Radaković M, Stevanović J, Đelić N, Lakić N, Knežević-Vukčević J, Vukovic-Gacić B, Stanimirović Z. Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences. 2013;38(1):53-62. doi:10.1007/s12038-012-9287-2 .
Radaković, Milena, Stevanović, Jevrosima, Đelić, Ninoslav, Lakić, Nada, Knežević-Vukčević, Jelena, Vukovic-Gacić, Branka, Stanimirović, Zoran, "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay" in Journal of Biosciences, 38, no. 1 (2013):53-62, https://doi.org/10.1007/s12038-012-9287-2 . .