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dc.creatorRadaković, Milena
dc.creatorStevanović, Jevrosima
dc.creatorĐelić, Ninoslav
dc.creatorLakić, Nada
dc.creatorKnežević-Vukčević, Jelena
dc.creatorVukovic-Gacić, Branka
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T13:44:06Z
dc.date.available2020-06-03T13:44:06Z
dc.date.issued2013
dc.identifier.issn0250-5991
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1037
dc.description.abstractAmitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.en
dc.publisherIndian Acad Sciences, Bangalore
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Biosciences
dc.subjectAmitrazen
dc.subjectapoptosisen
dc.subjectcatalaseen
dc.subjectComet assayen
dc.subjectDNA damageen
dc.titleEvaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assayen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадаковић, Милена; Ђелић, Нинослав; Лакић, Нада; Кнежевић-Вукчевић, Јелена; Вуковиц-Гацић, Бранка; Станимировић, Зоран; Стевановић, Јевросима;
dc.citation.volume38
dc.citation.issue1
dc.citation.spage53
dc.citation.epage62
dc.citation.other38(1): 53-62
dc.citation.rankM21
dc.identifier.wos000314749100009
dc.identifier.doi10.1007/s12038-012-9287-2
dc.identifier.pmid23385813
dc.identifier.scopus2-s2.0-84873715822
dc.identifier.rcubconv_1893
dc.type.versionpublishedVersion


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