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Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava

dc.creatorMaletić, Milan
dc.creatorKanjac, Slobodanka
dc.creatorĐelić, Ninoslav
dc.creatorLakić, Nada
dc.creatorPavlović, Miloš
dc.creatorNedić, Svetlana
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T13:45:28Z
dc.date.available2020-06-03T13:45:28Z
dc.date.issued2013
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1057
dc.description.abstractLactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes.en
dc.description.abstractLaktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectcow somatic cellsen
dc.subjectlactoferrin geneen
dc.subjectmammary glanden
dc.subjectPCR-RFLPen
dc.subjectpolymorphismen
dc.titleAnalysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cowsen
dc.titleAnaliza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih kravasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНедић, Светлана; Павловић, Милош; Станимировић, Зоран; Лакић, Нада; Малетић, Милан; Кањац, Слободанка; Ђелић, Нинослав; Aнализа полиморфизма лактоферин гена и његова повезаност са квалитетом млека и здрављем млечне жлезде код Холштајн-Фризијских крава; Aнализа полиморфизма лактоферин гена и његова повезаност са квалитетом млека и здрављем млечне жлезде код Холштајн-Фризијских крава;
dc.citation.volume63
dc.citation.issue5-6
dc.citation.spage487
dc.citation.epage498
dc.citation.other63(5-6): 487-498
dc.citation.rankM23
dc.identifier.wos000329380800002
dc.identifier.doi10.2298/AVB1306487M
dc.identifier.scopus2-s2.0-84894320139
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/52/1056.pdf
dc.identifier.rcubconv_809
dc.type.versionpublishedVersion


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