Приказ основних података о документу
Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows
Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava
dc.creator | Maletić, Milan | |
dc.creator | Vakanjac, Slobodanka | |
dc.creator | Đelić, Ninoslav | |
dc.creator | Lakić, Nada | |
dc.creator | Pavlović, Miloš | |
dc.creator | Nedić, Svetlana | |
dc.creator | Stanimirović, Zoran | |
dc.date.accessioned | 2020-06-03T13:45:28Z | |
dc.date.available | 2020-06-03T13:45:28Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1057 | |
dc.description.abstract | Lactoferrin (LTF) is a glycoprotein, a member of transferrin gene family which plays an important role in immune mechanisms in the mammary glands of cows. The amount of lactoferrin increases during inflammatory processes and viral infections. The aim of this investigation was to monitor the distribution of lactoferrin gene genotypes and its connection to milk quality and the occurrence of mammary gland diseases in 46 Holstein-Freisian cows of different age (2-7 years) on a farm near Belgrade. DNA was isolated from blood samples, and the polymorphism of lactoferrin gene was deterimined by PCR-RFLP method using the restriction enzyme Eco RI. We found two alelic forms of this gene in cows included in these experiments (A and B) and two genotypes (AA and AB) in a ratio 71.7% to 28.3%. The genotype BB was not found in this sample. In order to determine the degree of differences between genotypes we used discriminant analysis which has shown that there is a statistically significant difference between genotypes AA and AB with respect to productive parameters. When analyzed separately, the only parameter which differed significantly (p=0.021) between two genotypes was total milk production. Individuals with observed genotypes are most similar for the amount of milk fat (p=0.271). There is no statistically significant difference in the number of somatic cells in milk samples between the examined genotypes. | en |
dc.description.abstract | Laktoferin (LTF) je glikoprotein, član familije transferina i igra važnu ulogu u odbrambenom mehanizmu mlečne žlezde krava. Količina cartoferina raste tokom inflamatornog procesa i virusne infekcije. Cilj ovog rada je bilo praćenje distribucije genotipova laktoferin gena i njihova povezanost sa kvalitetom mleka i pojavom oboljenja mlečne žlezde kod 46 krava Holštajn-Frizijske rase, različite starosti (2-7 godina) na farmi u blizini Beograda. Obavljena je izolacija DNK iz krvi, a polimorfizam laktoferin gena utvrđen je PCR-RFLP metodom pomoću restrikcionog enzima Eco RI. Kod životinja uključenih u ogled utvrđene su dve alelne forme, A i B i dva genotipa, AA i AB u odnosu 71,74% prema 28,26%. Genotip BB nije pronađen u datom uzorku životinja. U cilju utvrđivanja stepena razdvajanja genotipova primenjena je diskriminaciona analiza koja je ukazala da se genotipovi AA i AB statistički značajno razlikuju prema proizvodnim parametrima posmatranim istovremeno. Pojedinačno, samo se po parametru ukupna proizvodnja mleka, krava analiziranih genotipova statistički značajno razlikuju (p=0.021). Grla posmatranih genotipova su najsličnija po količini mlečne masti (p=0.271). Nije ustanovljena statistički značajna razlika u broju somatskih ćelija u mleku između ispitivanih genotipova. | sr |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS// | |
dc.rights | openAccess | |
dc.source | Acta Veterinaria-Beograd | |
dc.subject | cow somatic cells | en |
dc.subject | lactoferrin gene | en |
dc.subject | mammary gland | en |
dc.subject | PCR-RFLP | en |
dc.subject | polymorphism | en |
dc.title | Analysis of lactoferin gene polymophism and its association to milk quality and mammary gland health in Holstein-Friesian cows | en |
dc.title | Analiza polimorfizma laktoferin gena i njegova povezanost sa kvalitetom mleka i zdravljem mlečne žlezde kod Holštajn-Frizijskih krava | sr |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Недић, Светлана; Павловић, Милош; Станимировић, Зоран; Лакић, Нада; Малетић, Милан; Кањац, Слободанка; Ђелић, Нинослав; Aнализа полиморфизма лактоферин гена и његова повезаност са квалитетом млека и здрављем млечне жлезде код Холштајн-Фризијских крава; Aнализа полиморфизма лактоферин гена и његова повезаност са квалитетом млека и здрављем млечне жлезде код Холштајн-Фризијских крава; | |
dc.citation.volume | 63 | |
dc.citation.issue | 5-6 | |
dc.citation.spage | 487 | |
dc.citation.epage | 498 | |
dc.citation.other | 63(5-6): 487-498 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000329380800002 | |
dc.identifier.doi | 10.2298/AVB1306487M | |
dc.identifier.scopus | 2-s2.0-84894320139 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/52/1056.pdf | |
dc.type.version | publishedVersion |