The influence of antisperm Ig G and Ig A antibodies from cows sera and cervical mucus on bull sperm motility

Abstract

The aim of this study was to investigate the influence of antisperm Ig G and Ig A antibodies (ASA) from the sera and cervical mucus of cows on bulls sperm motility. A total of 64 cows was included in the study and samples of sera and cervical mucus were collected on the day of artificial insemination. Cows were of Busha breed or mix breed with Simmental. The presence of antisperm Ig G and Ig A antibodies was determined by indirect immunofluorescence method and according to these results, cows were divided in groups as follows: cows with high or low ASA titer in their sera and cows with high or low ASA titer in the cervical mucus. Influence of antisperm antibodies on sperm motility was further estimated by Computer Assisted Semen Analysis (CASA). Results demonstrated a significant difference in the influence of antisperm antibodies depending on their origin and titer.

Osnovni cilj ovih istraživanja je bio da se ispita uticaj antitela Ig G i Ig A klase protiv antigena spermatozoida (ASA) iz krvnog seruma i cervikalne sluzi krava na pokretljivost spermatozoida bika. Studijom je bilo obuhvaćeno 64 plotkinje a uzorci seruma i cervikalne sluzi su prikupljani na dan veštačkog osemenjavanja. Krave su bile rase buša ili melezi buše sa simentalcem. Titar antitela Ig G i Ig A klase je određivan metodom indirektne imunofluorescence i na osnovu tih rezultata krave su bile podeljene u sledeće grupe: plotkinje sa visokim i niskim titrom ASA u krvnom serumu i plotkinje sa visokim i niskim titrom ASA u cervikalnoj sluzi. Uticaj ASA na pokretljivost spermatozoida je zatim određivan kompjuterskom analizom (Computer Assisted Semen Analisis - CASA). Dobijeni rezultati ukazuju značajne razlike u uticaju ASA na pokretljivost spermatozoida u zavisnosti od njihovog porekla i titra.