Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina
2013
Authors
Dimitrijević, VladimirStevanović, Jevrosima
Savić, Mila
Petrujkić, Branko
Simeunović, Predrag
Milošević, Ivan
Stanimirović, Zoran
Article (Published version)
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The aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs.... In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed.
Keywords:
dog / canine microsatellites / allele frequency / parentage testingSource:
Annals of Animal Science, 2013, 13, 4, 715-722Publisher:
- Walter De Gruyter Gmbh, Berlin
Funding / projects:
DOI: 10.2478/aoas-2013-0047
ISSN: 2300-8733
WoS: 000326082600005
Scopus: 2-s2.0-84887240551
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Fakultet veterinarske medicineTY - JOUR AU - Dimitrijević, Vladimir AU - Stevanović, Jevrosima AU - Savić, Mila AU - Petrujkić, Branko AU - Simeunović, Predrag AU - Milošević, Ivan AU - Stanimirović, Zoran PY - 2013 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1061 AB - The aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs. In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed. PB - Walter De Gruyter Gmbh, Berlin T2 - Annals of Animal Science T1 - Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina VL - 13 IS - 4 SP - 715 EP - 722 DO - 10.2478/aoas-2013-0047 ER -
@article{ author = "Dimitrijević, Vladimir and Stevanović, Jevrosima and Savić, Mila and Petrujkić, Branko and Simeunović, Predrag and Milošević, Ivan and Stanimirović, Zoran", year = "2013", abstract = "The aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs. In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed.", publisher = "Walter De Gruyter Gmbh, Berlin", journal = "Annals of Animal Science", title = "Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina", volume = "13", number = "4", pages = "715-722", doi = "10.2478/aoas-2013-0047" }
Dimitrijević, V., Stevanović, J., Savić, M., Petrujkić, B., Simeunović, P., Milošević, I.,& Stanimirović, Z.. (2013). Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina. in Annals of Animal Science Walter De Gruyter Gmbh, Berlin., 13(4), 715-722. https://doi.org/10.2478/aoas-2013-0047
Dimitrijević V, Stevanović J, Savić M, Petrujkić B, Simeunović P, Milošević I, Stanimirović Z. Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina. in Annals of Animal Science. 2013;13(4):715-722. doi:10.2478/aoas-2013-0047 .
Dimitrijević, Vladimir, Stevanović, Jevrosima, Savić, Mila, Petrujkić, Branko, Simeunović, Predrag, Milošević, Ivan, Stanimirović, Zoran, "Validation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplanina" in Annals of Animal Science, 13, no. 4 (2013):715-722, https://doi.org/10.2478/aoas-2013-0047 . .