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dc.creatorDimitrijević, Vladimir
dc.creatorStevanović, Jevrosima
dc.creatorSavić, Mila
dc.creatorPetrujkić, Branko
dc.creatorSimeunović, Predrag
dc.creatorMilošević, Ivan
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T13:45:44Z
dc.date.available2020-06-03T13:45:44Z
dc.date.issued2013
dc.identifier.issn2300-8733
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1061
dc.description.abstractThe aim of the study was to assess a commercially available microsatellite panel for use in paternity and identification analyses in the Yugoslavian Shepherd Dog Sharplanina, an ancient livestock guarding breed. Allele frequencies for 10 microsatellite loci (PEZ01, FHC2054, FHC2010, PEZ05, PEZ20, PEZ12, PEZ03, PEZ06, PEZ08 and FHC2079) were determined in 103 unrelated Yugoslavian Shepherd Dog individuals. The loci revealed varied levels of polymorphism (five to 12 alleles), with an average of 7.83 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.64 and 0.66, respectively. Nine out of 10 microsatellite markers were highly informative with PIC values higher than 0.5. The obtained value of combined power of exclusion (0.9989) confirms usefulness of this panel of microsatellites for parentage verification, while the value of combined power of discrimination of 0.9999 clearly shows that the panel can conclusively identify individual dogs. In conclusion, the results obtained suggest that the selected set of commercially available microsatellite markers may be used as a routine tool for parentage verification and individual identification in the Yugoslavian Shepherd Dog. Although analysis of genetic variability of the Yugoslavian Shepherd Dog was not the primary focus of the study, the established values of major diversity indices disclose a highly variable gene pool in the breed.en
dc.publisherWalter De Gruyter Gmbh, Berlin
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceAnnals of Animal Science
dc.subjectdogen
dc.subjectcanine microsatellitesen
dc.subjectallele frequencyen
dc.subjectparentage testingen
dc.titleValidation of 10 microsatellite loci for their use in parentage verification and individual identification in the Yugoslavian Shepherd Dog Sharplaninaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДимитријевић, Владимир; Станимировић, Зоран; Милошевић, Иван; Савић, Мила; Стевановић, Јевросима; Симеуновић, Предраг; Петрујкић, Бранко;
dc.citation.volume13
dc.citation.issue4
dc.citation.spage715
dc.citation.epage722
dc.citation.other13(4): 715-722
dc.identifier.wos000326082600005
dc.identifier.doi10.2478/aoas-2013-0047
dc.identifier.scopus2-s2.0-84887240551
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/57/1060.pdf
dc.identifier.rcubconv_1942
dc.type.versionpublishedVersion


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