Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2
Abstract
The objective of our study was to check the immunogenicity of experimental bivalent subunit vaccines against Herpes simplex viruses (HSV1 and HSV2). The subunit vaccines were prepared from glycoprotein antigens isolated from the external envelopes of HSV1 and HSV2. In the first vaccine the glycoproteins subunits were adsorbed on the adjuvant Al(OH)3. The secortd subunit vaccine was prepared from glycoprotein antigens with the adjuvant MPs-19 (a water soluble oligosaccharide) dissolved in phosphate buffered saline (PBS). The final concentration of Al(OH)3 in the first vaccine was 5mg/ml, while the final concentration of MPS-(19) in the second vaccine was 2 mg/ml. These vaccines contained 0.13 mg total protein for the HSV1 subunits and 0.16 mg for the HSV2 subunits ml. One group of 10 female Swiss albino mice (20 gr body weight) were each immunized s.c. with 0.5 ml of the HSV1/HSV2 subunit vaccine, containing the adjuvant Al(OH)3. The second group of 10 mice was immunized with the same q...uantity of the HSV1/HSV2 subunit vaccine with adjuvant MPS-19. A group II healthy nonimmunized mice served as the control in the assay. The specific humoral immune response of the vaccinated mice was measured by a standard method of indirect immunofluorescence. The specific cellular immune response of the vaccinated animals was tested by measuring the lymphocyte proliferation response to HSV1 and HSV2 antigens with tritium methyl thymidine *128.5 Bq/mmol), in vitro. The titres of the specific IgG and IgM class antibodies, in the sera of both groups of immunized mice on day 10 after vaccination were: a) with adjuvant Al(OH)3: from 1.8 to 1:128 IgG; and 1:32 to 1:128 IgM for HSV1; from 1:32 to 1:128 IgG and IgM antibodies, for HSV2; b) with adjuvant MPS-(19); from 1.16 to 1:128 IgG and 1:64 to 1:128 IgM for HSV1; from 1:16 to 1:32 IgG and 1:64 to 1:128 IgM for HSV2. The mean values for radioactivity in the medium after spontaneous proliferation of lymphocytes were 799 cpm for the first and 798 cpm for the second experimental group. The mean radioactivity of lymphocytes from the first group of mice stimulated with HSV1 and HSV2 antigens in, 10 days after immunization, was 1470 cpm (for HSV1) and 1845 cpm (for HSV2). The mean values of radioactivity in the proliferation test for HSV1 and HSV2 antigens in the second (for HSV1) and 2416 cpm (for HSV2). Thus, the low subunit antigen concentrations in both vaccines, induced satisfacton/humoral and cellular immune responses to HSV1 and HSV2 in the organisms of immunized mice.
Keywords:
HSV1 / HSV2 glycoprotein antigens / subunit vaccines / immunization / swiss albino miceSource:
Acta Veterinaria-Beograd, 1999, 49, 1, 3-11Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
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Fakultet veterinarske medicineTY - JOUR AU - Milić, Nenad AU - Jovanović, T AU - Knežević, I AU - Gađanski-Omerović, Gordana AU - Ašanin, Ružica PY - 1999 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/107 AB - The objective of our study was to check the immunogenicity of experimental bivalent subunit vaccines against Herpes simplex viruses (HSV1 and HSV2). The subunit vaccines were prepared from glycoprotein antigens isolated from the external envelopes of HSV1 and HSV2. In the first vaccine the glycoproteins subunits were adsorbed on the adjuvant Al(OH)3. The secortd subunit vaccine was prepared from glycoprotein antigens with the adjuvant MPs-19 (a water soluble oligosaccharide) dissolved in phosphate buffered saline (PBS). The final concentration of Al(OH)3 in the first vaccine was 5mg/ml, while the final concentration of MPS-(19) in the second vaccine was 2 mg/ml. These vaccines contained 0.13 mg total protein for the HSV1 subunits and 0.16 mg for the HSV2 subunits ml. One group of 10 female Swiss albino mice (20 gr body weight) were each immunized s.c. with 0.5 ml of the HSV1/HSV2 subunit vaccine, containing the adjuvant Al(OH)3. The second group of 10 mice was immunized with the same quantity of the HSV1/HSV2 subunit vaccine with adjuvant MPS-19. A group II healthy nonimmunized mice served as the control in the assay. The specific humoral immune response of the vaccinated mice was measured by a standard method of indirect immunofluorescence. The specific cellular immune response of the vaccinated animals was tested by measuring the lymphocyte proliferation response to HSV1 and HSV2 antigens with tritium methyl thymidine *128.5 Bq/mmol), in vitro. The titres of the specific IgG and IgM class antibodies, in the sera of both groups of immunized mice on day 10 after vaccination were: a) with adjuvant Al(OH)3: from 1.8 to 1:128 IgG; and 1:32 to 1:128 IgM for HSV1; from 1:32 to 1:128 IgG and IgM antibodies, for HSV2; b) with adjuvant MPS-(19); from 1.16 to 1:128 IgG and 1:64 to 1:128 IgM for HSV1; from 1:16 to 1:32 IgG and 1:64 to 1:128 IgM for HSV2. The mean values for radioactivity in the medium after spontaneous proliferation of lymphocytes were 799 cpm for the first and 798 cpm for the second experimental group. The mean radioactivity of lymphocytes from the first group of mice stimulated with HSV1 and HSV2 antigens in, 10 days after immunization, was 1470 cpm (for HSV1) and 1845 cpm (for HSV2). The mean values of radioactivity in the proliferation test for HSV1 and HSV2 antigens in the second (for HSV1) and 2416 cpm (for HSV2). Thus, the low subunit antigen concentrations in both vaccines, induced satisfacton/humoral and cellular immune responses to HSV1 and HSV2 in the organisms of immunized mice. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2 VL - 49 IS - 1 SP - 3 EP - 11 UR - https://hdl.handle.net/21.15107/rcub_veterinar_107 ER -
@article{ author = "Milić, Nenad and Jovanović, T and Knežević, I and Gađanski-Omerović, Gordana and Ašanin, Ružica", year = "1999", abstract = "The objective of our study was to check the immunogenicity of experimental bivalent subunit vaccines against Herpes simplex viruses (HSV1 and HSV2). The subunit vaccines were prepared from glycoprotein antigens isolated from the external envelopes of HSV1 and HSV2. In the first vaccine the glycoproteins subunits were adsorbed on the adjuvant Al(OH)3. The secortd subunit vaccine was prepared from glycoprotein antigens with the adjuvant MPs-19 (a water soluble oligosaccharide) dissolved in phosphate buffered saline (PBS). The final concentration of Al(OH)3 in the first vaccine was 5mg/ml, while the final concentration of MPS-(19) in the second vaccine was 2 mg/ml. These vaccines contained 0.13 mg total protein for the HSV1 subunits and 0.16 mg for the HSV2 subunits ml. One group of 10 female Swiss albino mice (20 gr body weight) were each immunized s.c. with 0.5 ml of the HSV1/HSV2 subunit vaccine, containing the adjuvant Al(OH)3. The second group of 10 mice was immunized with the same quantity of the HSV1/HSV2 subunit vaccine with adjuvant MPS-19. A group II healthy nonimmunized mice served as the control in the assay. The specific humoral immune response of the vaccinated mice was measured by a standard method of indirect immunofluorescence. The specific cellular immune response of the vaccinated animals was tested by measuring the lymphocyte proliferation response to HSV1 and HSV2 antigens with tritium methyl thymidine *128.5 Bq/mmol), in vitro. The titres of the specific IgG and IgM class antibodies, in the sera of both groups of immunized mice on day 10 after vaccination were: a) with adjuvant Al(OH)3: from 1.8 to 1:128 IgG; and 1:32 to 1:128 IgM for HSV1; from 1:32 to 1:128 IgG and IgM antibodies, for HSV2; b) with adjuvant MPS-(19); from 1.16 to 1:128 IgG and 1:64 to 1:128 IgM for HSV1; from 1:16 to 1:32 IgG and 1:64 to 1:128 IgM for HSV2. The mean values for radioactivity in the medium after spontaneous proliferation of lymphocytes were 799 cpm for the first and 798 cpm for the second experimental group. The mean radioactivity of lymphocytes from the first group of mice stimulated with HSV1 and HSV2 antigens in, 10 days after immunization, was 1470 cpm (for HSV1) and 1845 cpm (for HSV2). The mean values of radioactivity in the proliferation test for HSV1 and HSV2 antigens in the second (for HSV1) and 2416 cpm (for HSV2). Thus, the low subunit antigen concentrations in both vaccines, induced satisfacton/humoral and cellular immune responses to HSV1 and HSV2 in the organisms of immunized mice.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2", volume = "49", number = "1", pages = "3-11", url = "https://hdl.handle.net/21.15107/rcub_veterinar_107" }
Milić, N., Jovanović, T., Knežević, I., Gađanski-Omerović, G.,& Ašanin, R.. (1999). Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 49(1), 3-11. https://hdl.handle.net/21.15107/rcub_veterinar_107
Milić N, Jovanović T, Knežević I, Gađanski-Omerović G, Ašanin R. Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2. in Acta Veterinaria-Beograd. 1999;49(1):3-11. https://hdl.handle.net/21.15107/rcub_veterinar_107 .
Milić, Nenad, Jovanović, T, Knežević, I, Gađanski-Omerović, Gordana, Ašanin, Ružica, "Examinations of the immunogenicity of the experimental bivalent subunit vaccines against herpes simplex virus type 1 and 2" in Acta Veterinaria-Beograd, 49, no. 1 (1999):3-11, https://hdl.handle.net/21.15107/rcub_veterinar_107 .