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dc.creatorVeljović, Ljubiša
dc.creatorKnežević, Aleksandra
dc.creatorMilić, Nenad
dc.creatorNišavić, Jakov
dc.date.accessioned2020-06-03T13:47:53Z
dc.date.available2020-06-03T13:47:53Z
dc.date.issued2014
dc.identifier.issn0354-4664
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1092
dc.description.abstractBovine parainfluenza 3 virus (PI3) causes respiratory infections in cattle and sheep with great economic losses in livestock. The aim of this investigation was to determine the significance of molecular methods in the identification of isolated strains of PI3 virus. Twenty cattle nasal swabs were analyzed for the presence of PI3 using the standard virology method of virus isolation in MBDK cell line and virus neutralization test. The identification of isolated strains was confirmed by RT-PCR and method of direct sequencing with primers for PI3 fusion (F) protein gene. PI3 virus was isolated and identified in four nasal swabs using the standard virology method and RT-PCR. The analysis of nucleotide sequences of isolated PI3 strains showed high similarity with sequences isolated from cattle in Asia. Our results showed that molecular methods are very useful in the diagnosis of PI3 infections as well as for the identification and characterization of PI3 strains in Serbia.en
dc.publisherSrpsko biološko društvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31008/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175073/RS//
dc.rightsopenAccess
dc.sourceArchives of Biological Sciences
dc.subjectBovine parainfluenza 3 virusen
dc.subjectRT-PCRen
dc.subjectdirect sequencingen
dc.titleThe application of molecular methods in the identification of isolated strains of parainfluenza 3 virus of cattleen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractНишавић, Јаков; Кнежевић, Aлександра; Милић, Ненад; Вељовић, Љубиша;
dc.citation.volume66
dc.citation.issue2
dc.citation.spage491
dc.citation.epage496
dc.citation.other66(2): 491-496
dc.citation.rankM23
dc.identifier.wos000342265800006
dc.identifier.doi10.2298/ABS1402491V
dc.identifier.scopus2-s2.0-84903952082
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/84/1091.pdf
dc.identifier.rcubconv_2012
dc.type.versionpublishedVersion


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