Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes
Samo za registrovane korisnike
2014
Autori
Čabarkapa, AndreaŽivković, Lada
Žukovec, Dijana
Đelić, Ninoslav
Bajić, Vladan
Dekanski, Dragana
Spremo-Potparević, Biljana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P < 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysi...s revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P < 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.
Ključne reči:
Dry olive leaf extract (DOLE), Adrenaline / (CAS 329-63-5) / Oxidative stress / Comet assayIzvor:
Toxicology in Vitro, 2014, 28, 3, 451-456Izdavač:
- Pergamon-Elsevier Science Ltd, Oxford
Finansiranje / projekti:
- Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.1016/j.tiv.2013.12.014
ISSN: 0887-2333
PubMed: 24389114
WoS: 000332053800015
Scopus: 2-s2.0-84892897826
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Čabarkapa, Andrea AU - Živković, Lada AU - Žukovec, Dijana AU - Đelić, Ninoslav AU - Bajić, Vladan AU - Dekanski, Dragana AU - Spremo-Potparević, Biljana PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1104 AB - Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P < 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P < 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Toxicology in Vitro T1 - Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes VL - 28 IS - 3 SP - 451 EP - 456 DO - 10.1016/j.tiv.2013.12.014 ER -
@article{ author = "Čabarkapa, Andrea and Živković, Lada and Žukovec, Dijana and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Spremo-Potparević, Biljana", year = "2014", abstract = "Excessive release of stress hormone adrenaline is accompanied by generation of reactive oxygen species which may cause disruption of DNA integrity leading to cancer and age-related disorders. Phenolic-rich plant product dry olive leaf extract (DOLE) is known to modulate effects of various oxidants in human cells. The aim was to evaluate the effect of commercial DOLE against adrenaline induced DNA damage in human leukocytes by using comet assay. Peripheral blood leukocytes from 6 healthy subjects were treated in vitro with three final concentrations of DOLE (0.125, 0.5, and 1 mg/mL) for 30 min at 37 degrees C under two different protocols, pretreatment and post-treatment. Protective effect of DOLE was assessed from its ability to attenuate formation of DNA lesions induced by adrenaline. Compared to cells exposed only to adrenaline, DOLE displayed significant reduction (P < 0.001) of DNA damage at all three concentrations and under both experimental protocols. Pearson correlation analysis revealed a significant positive association between DOLE concentration and leukocytes DNA damage (P < 0.05). Antigenotoxic effect of the extract was more pronounced at smaller concentrations. Post-treatment with 0.125 mg/mL DOLE was the most effective against adrenaline genotoxicity. Results indicate genoprotective and antioxidant properties in dry olive leaf extract, strongly supporting further explorations of its underlying mechanisms of action.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Toxicology in Vitro", title = "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes", volume = "28", number = "3", pages = "451-456", doi = "10.1016/j.tiv.2013.12.014" }
Čabarkapa, A., Živković, L., Žukovec, D., Đelić, N., Bajić, V., Dekanski, D.,& Spremo-Potparević, B.. (2014). Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology in Vitro Pergamon-Elsevier Science Ltd, Oxford., 28(3), 451-456. https://doi.org/10.1016/j.tiv.2013.12.014
Čabarkapa A, Živković L, Žukovec D, Đelić N, Bajić V, Dekanski D, Spremo-Potparević B. Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes. in Toxicology in Vitro. 2014;28(3):451-456. doi:10.1016/j.tiv.2013.12.014 .
Čabarkapa, Andrea, Živković, Lada, Žukovec, Dijana, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Spremo-Potparević, Biljana, "Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes" in Toxicology in Vitro, 28, no. 3 (2014):451-456, https://doi.org/10.1016/j.tiv.2013.12.014 . .