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dc.creatorNešić, Ksenija
dc.creatorŠamanc, Horea
dc.creatorVujanac, Ivan
dc.creatorProdanović, Radiša
dc.creatorNešić, Vladimir
dc.creatorVelebit, Branko
dc.creatorSavić, Božidar
dc.date.accessioned2020-06-03T13:49:10Z
dc.date.available2020-06-03T13:49:10Z
dc.date.issued2014
dc.identifier.issn0377-8401
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1111
dc.description.abstractThe ban of processed animal proteins (PAP) in feed for farmed animals led to a significant reduction of the number of BSE cases ever since bovine spongiform encephalopathy (BSE) was proven to be a feedborne disease. Although optical microscopy has been the only reference method for the detection of PAP for years, the EU legislation also foresees that other methods may be applied in addition to the microscopy, if they provide appropriate information about the origin of the animal constituents present in animal feed. This was set to become a reality in the European Union from the 1st of June 2013 when meat and bone meal (MBM) was reintroduced in fish feed. This paper presents a comparison and the possibility of combining classical microscopy and conventional polymerase chain reaction (PCR), as complementary techniques, implemented to detect the presence of fish meal and bovine, poultry, pig and mixed MBM in cattle feed. Both methods were also applied on the samples of ruminal fluid of cows fed feed containing MBM. The ruminal fluid was chosen to be tested in order to determine its forensic significance, as it is often not possible to perform the analysis of the incrimiated feed that has already been consumed. In contrast to the high sensitivity of microscopy, the results obtained by PCR showed substantial variability, even when applied to the isolated bones. Particles of animal origin in ruminal fluid were detected only by microscopy.en
dc.publisherElsevier, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
dc.rightsrestrictedAccess
dc.sourceAnimal Feed Science and Technology
dc.subjectBovine spongiform encephalopathyen
dc.subjectFeed controlen
dc.subjectProcessed animal proteinsen
dc.titleDetection of meat and bone meal in cattle feed and ruminal fluid - Comparison and combining of microscopy and polymerase chain reactionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВелебит, Бранко; Шаманц, Хореа; Вујанац, Иван; Продановић, Радиша; Савић, Б.; Нешић, Ксенија; Нешић, Владимир;
dc.citation.volume187
dc.citation.spage86
dc.citation.epage90
dc.citation.other187: 86-90
dc.citation.rankM21
dc.identifier.wos000329952800010
dc.identifier.doi10.1016/j.anifeedsci.2013.11.001
dc.identifier.scopus2-s2.0-84890803761
dc.type.versionpublishedVersion


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Приказ основних података о документу